| 1. |
- Tscheligi, M., et al.
(author)
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"Touch me" - Workshop on tactile user experience evaluation methods
- 2014
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In: Proceeding CHI EA '14 CHI '14 Extended Abstracts on Human Factors in Computing Systems. - New York, NY, USA : Association for Computing Machinery (ACM). - 9781450324748 ; , s. 41-44
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Conference paper (peer-reviewed)abstract
- In this workshop we plan to explore the possibilities and challenges of physical objects and materials for evaluating the User Experience (UX) of interactive systems. These objects should face shortfalls of current UX evaluation methods and allow for a qualitative (or even quantitative), playful and holistic evaluation of UX - without interfering with the users' personal experiences during interaction. This provides a tactile enhancement to a solely visual stimulation as used in classical evaluation methods. The workshop serves as a basis for networking and community building with interested HCI researchers, designers and practitioners and should encourage further development of the field of tactile UX evaluation.
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| 2. |
- Dahlin, Andreas, et al.
(author)
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Localized Surface Plasmon Resonance Sensing of Lipid-Membrane-Mediated Biorecognition Events
- 2005
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In: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 127:14, s. 5043-5048
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Journal article (peer-reviewed)abstract
- Supported phospholipid bilayers (SPBs) have emerged as important model systems for studies of the natural cell membrane and its components, which are essential for the integrity and function of cells in all living organisms, and also constitute common targets for therapeutic drugs and in disease diagnosis. However, the preferential occurrence of spontaneous SPB formation on silicon-based substrates, but not on bare noble-metal surfaces, has so far excluded the use of the localized surface plasmon resonance (LSPR) sensing principle for studies of lipid-membrane-mediated biorecognition reactions. This is because the LSPR phenomenon is associated with, and strongly confined to, the interfacial region of nanometric noble-metal particles. This problem has been overcome in this study by a self-assembly process utilizing localized rupture of phospholipid vesicles on silicon dioxide in the bottom of nanometric holes in a thin gold film. The hole-induced localization of the LSPR field to the voids of the holes is demonstrated to provide an extension of the LSPR sensing concept to studies of reactions confined exclusively to SPB-patches supported on SiO2. In particular, we emphasize the possibility of performing label-free studies of lipid-membrane-mediated reaction kinetics, including the compatibility of the assay with array-based reading (similar to 7 x 7 mu m(2)) and detection of signals originating from bound protein in the zeptomole regime.
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| 3. |
- Hofmann, K. B., et al.
(author)
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The influence of sitting posture on mechanics and metabolic energy requirements during sit-skiing : a case report
- 2016
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In: Sports Engineering. - : Springer Science and Business Media LLC. - 1369-7072 .- 1460-2687. ; 19:3, s. 213-218
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Journal article (peer-reviewed)abstract
- Several different sitting postures are used in Paralympic cross-country sit-skiing. The aim of this study was to evaluate the impact of sitting posture on physiological and mechanical variables during steady-state double-poling sit-skiing, as well as to determine how seat design can be improved for athletes without sufficient trunk control. Employing a novel, custom-designed seat, three trunk positions were tested while performing double-poling with submaximal oxygen consumption on an ergometer. Cycle kinematics, pole forces, and oxygen consumption were monitored. The athlete performed best, with longer cycle length and less pronounced metabolic responses, when kneeling with the trunk resting on a frontal support. For this case, a forward leaning trunk with knees below the hip joint was interpreted as most optimal, as it showed lower oxygen consumption and related parameters of performance during cross-country sit-skiing. Further investigations should examine whether such improvement is dependent on the level of the athlete’s handicap, as well as whether it is also seen on snow.
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| 4. |
- Kerne, Andruid, et al.
(author)
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Evaluation Methods for Creativity Support Environments
- 2013
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In: Conference on Human Factors in Computing Systems - Proceedings. - New York, NY, USA : Association for Computing Machinery. - 9781450318990 ; , s. 3295-3298
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Conference paper (peer-reviewed)abstract
- Creativity refers to the human processes that underpin sublime forms of expression and fuel innovation. Creativity support environments (CSEs) address diverse areas, such as education, science, business, disaster response, design, art, performance, and everyday life. A CSE may consist of a desktop application, or use specialized hardware, networked topologies, and mobile devices. CSEs may address temporal-spatial aspects of collaborative work. This workshop gathers a community of researchers developing and evaluating CSEs. We will share approaches, engage in dialogue, and develop best practices. The outcome is not a single prescription, but an ontology of methodologies with consideration to how they map to creative activities, and an emerging consensus on the range of expectations for rigorous evaluation to shape the field of CSE research. The workshop will organize an open repository of CSE evaluation methods and test data.
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| 5. |
- Lucero, A., et al.
(author)
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A sample of one : First-person research methods in HCI
- 2019
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In: DIS 2019 Companion - Companion Publication of the 2019 ACM Designing Interactive Systems Conference. - New York, NY, USA : Association for Computing Machinery (ACM). - 9781450362702 ; , s. 385-388
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Conference paper (peer-reviewed)abstract
- First-person research (i.e., research that involves data collection and experiences from the researcher themselves) continues to become a viable addition and, possibly even, alternative to more traditional HCI methods. While we have seen the benefits of using methods such as autoethnography, autobiographical design, and autoethnographical research through design, we also see the need to further explore, define, and investigate the practices, techniques, tactics, and implications of first-person research in HCI. To address this, this one-day workshop aims to bring together a community of researchers, designers, and practitioners who are interested in exploring and reimagining research in HCI and interaction design, with an emphasis on first-person methods.
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| 6. |
- Avila, J. M., et al.
(author)
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Soma design for nime
- 2020
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In: Proceedings of the International Conference on New Interfaces for Musical Expression. - : International Conference on New Interfaces for Musical Expression. ; , s. 489-494
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Conference paper (peer-reviewed)abstract
- Previous research on musical embodiment has reported that expert performers often regard their instruments as an extension of their body. Not every digital musical instrument seeks to create a close relationship between body and instrument, but even for the many that do, the design process often focuses heavily on technical and sonic factors, with relatively less attention to the bodily experience of the performer. In this paper we propose soma design as an alternative approach to explore this space. Soma method aims to attune the sensibilities of designers, as well as their experience of their body, and make use of these notions as a resource for creative aesthetic design. We report on a series of workshops exploring the relationship between the body and the guitar with a soma design approach. The workshops resulted in a series of guitar-related artefacts and NIMEs that emerged from the somatic exploration of balance and tension during guitar performance. Lastly we present lessons learned from our research that could inform future Soma-based musical instrument design, and how NIME research may also inform soma design.
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| 7. |
- Carlred, Louise M, 1985, et al.
(author)
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Imaging of Amyloid-β in Alzheimer’s disease transgenic mouse brains with Time-of-Flight Secondary Ion Mass Spectrometry using Immunoliposomes
- 2016
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In: Biointerphases. - : American Vacuum Society. - 1559-4106 .- 1934-8630. ; 11:2, s. 1-11
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Journal article (peer-reviewed)abstract
- Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been proven to successfully image different kinds of molecules, especially a variety of lipids, in biological samples. Proteins, however, are difficult to detect as specific entities with this method due to extensive fragmentation. To circumvent this issue, the authors present in this work a method developed for detection of proteins using antibody-conjugated liposomes, so called immunoliposomes, which are able to bind to the specific protein of interest. In combination with the capability of ToF-SIMS to detect native lipids in tissue samples, this method opens up the opportunity to analyze many different biomolecules, both lipids and proteins, at the same time, with high spatial resolution. The method has been applied to detect and image the distribution of amyloid-β (Aβ), a biologically relevant peptide in Alzheimer's disease (AD), in transgenic mouse braintissue. To ensure specific binding, the immunoliposome binding was verified on a model surface using quartz crystal microbalance with dissipation monitoring. The immunoliposome binding was also investigated on tissue sections with fluorescence microscopy, and compared with conventional immunohistochemistry using primary and secondary antibodies, demonstrating specific binding to Aβ. Using ToF-SIMS imaging, several endogenous lipids, such as cholesterol and sulfatides, were also detected in parallel with the immunoliposome-labeled Aβ deposits, which is an advantage compared to fluorescence microscopy. This method can thus potentially provide further information about lipid–protein interactions, which is important to understand the mechanisms of neurodegeneration in AD.
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| 8. |
- Carlred, Louise M, 1985, et al.
(author)
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Simultaneous imaging of amyloid-β and lipids in brain tissue using antibody-coupled liposomes and time-of-flight secondary ion mass spectrometry
- 2014
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In: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 136:28, s. 9973-9981
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Journal article (peer-reviewed)abstract
- The spatial localization of amyloid-β peptide deposits, the major component of senile plaques in Alzheimer's disease (AD), was mapped in transgenic AD mouse brains using time-of-flight secondary ion mass spectrometry (ToF-SIMS), simultaneously with several endogenous molecules that cannot be mapped using conventional immunohistochemistry imaging, including phospholipids, cholesterol and sulfatides. Whereas the endogenous lipids were detected directly, the amyloid-β deposits, which cannot be detected as intact entities with ToF-SIMS because of extensive ion-induced fragmentation, were identified by specific binding of deuterated liposomes to antibodies directed against amyloid-β. Comparative investigation of the amyloid-β deposits using conventional immunohistochemistry and fluorescence microscopy suggests similar sensitivity but a more surface-confined identification due to the shallow penetration depth of the ToF-SIMS signal. The recorded ToF-SIMS images thus display the localization of lipids and amyloid-β in a narrow (∼10 nm) two-dimensional plane at the tissue surface. As compared to a frozen nontreated tissue sample, the liposome preparation protocol generally increased the signal intensity of endogenous lipids, likely caused by matrix effects associated with the removal of salts, but no severe effects on the tissue integrity and the spatial distribution of lipids were observed with ToF-SIMS or scanning electron microscopy (SEM). This method may provide an important extension to conventional tissue imaging techniques to investigate the complex interplay of different kinds of molecules in neurodegenerative diseases, in the same specimen. However, limitations in target accessibility of the liposomes as well as unspecific binding need further consideration. © 2014 American Chemical Society.
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| 9. |
- Edvardsson, Malin, 1973, et al.
(author)
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Investigation of binding event perturbations caused by elevated QCM-D oscillation amplitude
- 2006
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In: Analyst. - : Royal Society of Chemistry (RSC). - 1364-5528. ; 131:7, s. 822-828
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Journal article (peer-reviewed)abstract
- We report measurements with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, with focus on how the shear oscillation amplitude of the sensor surface influences biorecognition binding events. Technically, this is made as reported recently ( M. Edvardsson, M. Rodahl, B. Kasemo, F. Hook, Anal. Chem., 2005, 77( 15), 4918-4926) by operating the QCM in dual frequency mode; one harmonic (n = n(1)) is utilized for continuous excitation of the QCM-D sensor at resonance at variable driving amplitudes (1-10 V), while the second harmonic (n not equal n(1)) is used for combined f and D measurements. By using one harmonic as a "probe" and the other one as an "actuator", elevated amplitudes can be used to perturb - or activate - binding reactions in a controlled way, while simultaneously maintaining the possibility of probing the adsorption and/or desorption events in a non-perturbative manner using combined f and D measurements. In this work we investigate the influence of oscillation amplitude variations on the binding of NeutrAvidin-modified polystyrene beads (O similar to 200 nm) to a planar biotin-modified lipid bilayer supported on an SiO2-modified QCM-D sensor. These results are further compared with data on an identical system, except that the NeutrAvidin-biotin recognition was replaced by fully complementary DNA hybridization. Supported by micrographs of the binding pattern, the results demonstrate that there exists, for both systems, a unique critical oscillation amplitude, A(c), below which binding is unaffected by the oscillation, and above which binding is efficiently prevented. Associated with A(c), there is a critical crystal radius, r(c), defining the central part of the crystal where binding is prevented. From QCM-D data, A(c) for the present system was estimated to be similar to 6.5 nm, yielding a value of r(c) of similar to 3 mm - the latter number was nicely confirmed by fluorescent- and dark-field micrographs of the crystal. Furthermore, the fact that A(c) is observed to be identical for the two types of biorecognition reactions suggests that it is neither the strength, nor the number of contact points, that determine the amplitude at which binding is prevented. Rather, particle size seems to be the determining parameter.
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| 10. |
- Fitzpatrick, G., et al.
(author)
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Daring to change : Creating a slower more sustainable academic life
- 2018
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In: Conference on Human Factors in Computing Systems - Proceedings. - New York, NY, USA : Association for Computing Machinery (ACM). - 9781450356206 - 9781450356213
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Conference paper (peer-reviewed)abstract
- Numerous reports and studies point to increasing performance criteria and workplace stress for academics/researchers. Together with the audience, this panel will explore how we experience this in the HCI community, focussing particularly on what we can do to change this for a slower more sustainable academic culture. The future of good quality HCI research is dependent on happy healthy researchers and reasonable realistic academic processes.
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