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- Fex, Malin, et al.
(author)
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A beta cell-specific knockout of hormone-sensitive lipase in mice results in hyperglycaemia and disruption of exocytosis.
- 2009
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In: Diabetologia. - : Springer Science and Business Media LLC. - 1432-0428 .- 0012-186X. ; 52, s. 271-280
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Journal article (peer-reviewed)abstract
- AIMS/HYPOTHESIS: The enzyme hormone-sensitive lipase (HSL) is produced and is active in pancreatic beta cells. Because lipids are known to play a crucial role in normal control of insulin release and in the deterioration of beta cell function, as observed in type 2 diabetes, actions of HSL in beta cells may be critical. This notion has been addressed in different lines of HSL knockout mice with contradictory results. METHODS: To resolve this, we created a transgenic mouse lacking HSL specifically in beta cells, and characterised this model with regard to glucose metabolism and insulin secretion, using both in vivo and in vitro methods. RESULTS: We found that fasting basal plasma glucose levels were significantly elevated in mice lacking HSL in beta cells. An IVGTT at 12 weeks revealed a blunting of the initial insulin response to glucose with delayed elimination of the sugar. Additionally, arginine-stimulated insulin secretion was markedly diminished in vivo. Investigation of the exocytotic response in single HSL-deficient beta cells showed an impaired response to depolarisation of the plasma membrane. Beta cell mass and islet insulin content were increased, suggesting a compensatory mechanism, by which beta cells lacking HSL strive to maintain normoglycaemia. CONCLUSIONS/INTERPRETATION: Based on these results, we suggest that HSL, which is located in close proximity of the secretory granules, may serve as provider of a lipid-derived signal essential for normal insulin secretion.
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| 3. |
- Villebrun, F., et al.
(author)
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Magnetic fields of intermediate-mass T Tauri stars : I. Magnetic detections and fundamental stellar parameters
- 2019
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In: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 622
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Journal article (peer-reviewed)abstract
- Context. The origin of the fossil magnetic fields detected in 5 to 10% of intermediate-mass main sequence stars is still highly debated.Aims. We want to bring observational constraints to a large population of intermediate-mass pre-main sequence (PMS) stars in order to test the theory that convective-dynamo fields generated during the PMS phases of stellar evolution can occasionally relax into fossil fields on the main sequence.Methods. Using distance estimations, photometric measurements, and spectropolarimetric data from HARPSpol and ESPaDOnS of 38 intermediate-mass PMS stars, we determined fundamental stellar parameters (T-eff, L and v sin i) and measured surface magnetic field characteristics (including detection limits for non-detections, and longitudinal fields and basic topologies for positive detections). Using PMS evolutionary models, we determined the mass, radius, and internal structure of these stars. We compared different PMS models to check that our determinations were not model-dependant. We then compared the magnetic characteristics of our sample accounting for their stellar parameters and internal structures.Results. We detect magnetic fields in about half of our sample. About 90% of the magnetic stars have outer convective envelopes larger than similar to 25% of the stellar radii, and heavier than similar to 2% of the stellar mass. Going to higher mass, we find that the magnetic incidence in intermediate-mass stars drops very quickly, within a timescale on the order of few times 0.1 Myr. Finally, we propose that intermediate-mass T Tauri stars with large convective envelopes, close to the fully convective limit, have complex fields and that their dipole component strengths may decrease as the sizes of their convective envelopes decrease, similar to lower-mass T Tauri stars.
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