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Search: WFRF:(Harnberg Anders)

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  • Magnusson, Anders O., et al. (author)
  • An S-selective lipase was created by rational redesign and the enantioselectivity increased with temperature
  • 2005
  • In: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 44:29, s. 4582-4585
  • Journal article (peer-reviewed)abstract
    • Higher activity with larger pockets: The figure shows a superposition of intermediates that occur in acyl transfer to (S)-1-phenylethanol catalyzed by Candida antarctica lipase B (CALB). Wild-type CALB cannot accomodate the phenyl group (gray) in the stereospecificity pocket and form all of the catalytically essential H bonds. The Trp 104 Ala mutation liberates the volume in yellow, the S enantiomer is easily fitted, and the specificity constant increases by a factor of 130 000.
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  • Result 1-1 of 1
Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Hult, Karl (1)
Takwa, Mohamad (1)
Magnusson, Anders O. (1)
Harnberg, Anders (1)
University
Royal Institute of Technology (1)
Language
English (1)
Year
2005 (1)

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