| 1. |
- Heuke, Sandro, et al.
(author)
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Fiber-based dual-focus time-demultiplexed second harmonic generation microscopy
- 2015
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In: Optics Letters. - 0146-9592. ; 40:11, s. 2505-2508
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Journal article (peer-reviewed)abstract
- We present a dual-focus second harmonic generation (SHG) microscopy approach based on stable, compact, and inexpensive fiber technology. One-tenth of the fiber laser output is coupled into a 100 m (=500 ns) long single-mode fiber and further amplified to achieve two separately guided beams with time-alternating pulse trains. SHG detection is performed sequentially, generating two individual images in one scan. Thus, the configuration allows for imaging of distinct areas within the field of view at twice the repetition rate of the fiber laser but is readily extended to a multiple of the repetition rate with tens of foci. (C) 2015 Optical Society of America
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| 2. |
- Legesse, Fisseha Bekele, et al.
(author)
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Dual-focus coherent anti-Stokes Raman scattering microscopy using a compact two-beam fiber laser source
- 2017
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In: Optics Letters. - 0146-9592. ; 42:2, s. 183-186
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Journal article (peer-reviewed)abstract
- We have developed a dual-focus coherent anti-Stokes Raman scattering (CARS) microscope based on a dual output, compact fiber laser source. The underlying concepts of time-multiplexed, two-beam scanning and demultiplexed detection that we already employed for second-harmonic generation are here naturally extended for CARS microscopy. The layout of a robust, all-fiber laser source was reconfigured to provide two outputs, each containing the two colors necessary for the CARS process. The utilization of the design for simultaneously imaging two laterally or axially separated fields of view and, thus, inherently speeding up the image acquisition process, is demonstrated on human artery tissue samples.
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