| 1. |
- Claesson, Per M., et al.
(författare)
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Interfacial properties of bottle-brush polyelectrolytes
- 2007
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Ingår i: PROCEEDINGS OF BALTIC POLYMER SYMPOSIUM 2007. - Vilnius : VILNIUS UNIV.. - 9789955331506 ; , s. 77-81
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Konferensbidrag (refereegranskat)abstract
- This article is focused on interfacial properties of bottle brush polyelectrolytes, where side-chains are attached along a polymer backbone. This class of polymer has been much less studied than block copolymers, which is particularly true for bottle brush polyelectrolytes with a high graft density. We have explored how the graft density and charge density of such polymers affect surface properties, as well as some bulk properties. The adsorption of this class of polymer onto negatively charged silica and mica surfaces has been determined. On mica adsorption is driven by electrostatic forces whereas on silica both electrostatic forces and interactions between silica and ethylene oxide chains drive the adsorption. On silica the adsorbed amount is very sensitive to solution ionic strength and pH. We also report on surface interactions and frictional forces obtained between surfaces coated with bottle brush polyelectrolytes.
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| 2. |
- Poulsen, Nicklas N., et al.
(författare)
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Automated coating procedures to produce poly(ethylene glycol) brushes in fused-silica capillaries
- 2017
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Ingår i: Journal of Separation Science. - : WILEY-V C H VERLAG GMBH. - 1615-9306 .- 1615-9314. ; 40:3, s. 779-788
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Tidskriftsartikel (refereegranskat)abstract
- Many bioanalytical methods rely on electrophoretic separation of structurally labile and surface active biomolecules such as proteins and peptides. Often poor separation efficiency is due to surface adsorption processes leading to protein denaturation and surface fouling in the separation channel. Flexible and reliable approaches for preventing unwanted protein adsorption in separation science are thus in high demand. We therefore present new coating approaches based on an automated in-capillary surface-initiated atom transfer radical polymerization process (covalent coating) aswell as by electrostatically adsorbing a presynthesized polymer leading to functionalized molecular brushes. The electroosmotic flow was measured following each step of the covalent coating procedure providing a detailed characterization and quality control. Both approaches resulted in good fouling resistance against the four model proteins cytochrome c, myoglobin, ovalbumin, and human serum albumin in the pH range 3.4-8.4. Further, even samples containing 10% v/v plasma derived from human blood did not show signs of adsorbing to the coated capillaries. The covalent as well as the electrostatically adsorbed coating were both found to be stable and provided almost complete suppression of the electroosmotic flow in the pH range 3.4-8.4. The coating procedures may easily be integrated in fully automated capillary electrophoresis methodologies.
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