| 1. |
- Alam, Md. Maksudul, et al.
(author)
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A Putative Leucine-Rich Repeat Receptor-Like Kinase of Jute Involved in Stress Response
- 2010
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In: Plant Molecular Biology Reporter. - : Springer Science and Business Media LLC. - 0735-9640 .- 1572-9818. ; 28:3, s. 394-402
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Journal article (peer-reviewed)abstract
- A putative leucine-rich repeat receptor-like protein kinase (LRR-RLK) gene together with its 5' and 3' untranslated regions of jute (Corchorus olitorius L.) has been identified and sequenced. The gene is 3,371 bp long containing two exons and one intron. The coding sequence of the gene is 2,879 bp long encoding a peptide of 957 amino acids. The predicted protein contains several domains and motifs characteristic of a transmembrane protein kinase. It is complete with domains for an N-terminal leucine-rich repeat and a protein kinase core, an active site for serine/threonine protein kinase, an ATP binding conserved site and a transmembrane region. Expression of the gene is induced by low temperature, high salt concentration, dehydration, abscisic acid treatment, and fungal infection, suggesting the involvement of the gene in multiple stress response pathways in jute (C. olitorius L.). A possible mechanism of the role of the gene in signal transduction and environmental stress response is discussed. To date, LRR-RLK is the only jute gene which has been completely sequenced and characterized.
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| 2. |
- Fagelskiold, Amanda Jabin, et al.
(author)
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Insulin-secreting INS-1E cells express functional TRPV1 channels
- 2012
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In: Islets. - : Informa UK Limited. - 1938-2014 .- 1938-2022. ; 4:1, s. 56-63
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Journal article (peer-reviewed)abstract
- We have studied whether functional TRPV1 channels exist in the INS-1E cells, a cell type used as a model for beta-cells, and in primary beta-cells from rat and human. The effects of the TRPV1 agonists capsaicin and AM404 on the intracellular free Ca2+ concentration ([Ca2+]i) in the INS-1E cells were studied by fura-2based microfluorometry. Capsaicin increased [Ca2+] i in a concentration-dependent manner, and the [Ca2+] i increase was dependent on extracellular Ca2+. AM404 also increased [Ca2+] i in the INS-1E cells. Capsazepine, a specific antagonist of TRPV1, completely blocked the capsaicin- and AM404-induced [Ca2+] i increases. Capsaicin did not increase [Ca2+] i in the primary beta-cells from rat and human. Whole cell patch clamp configuration was used to record currents across the plasma membrane in the INS-1E cells. Capsaicin elicited inward currents that were inhibited by capsazepine. Western blot analysis detected TRPV1 proteins in the INS-1E cells and the human islets. Immunohistochemistry was used to study the expression of TRPV1, but no TRPV1 protein immunoreactivity was detected in the human islet cells and the human insulinoma cells. We conclude that the INS-1E cells, but not the primary beta-cells, express functional TRPV1 channels.
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| 3. |
- Goswami, Chandan, et al.
(author)
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Transient Receptor Potential channels : What's happening? Reflections in the wake of the 2009 TRP Meeting, Karolinska Institutet, Stockholm
- 2010
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In: Channels (Austin, Tex.). - : Informa UK Limited. - 1933-6950 .- 1933-6969. ; 4:2, s. 124-135
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Journal article (peer-reviewed)abstract
- More than 150 participants from 25 countries gathered in Stockholm during 25(th) to 27(th) Sept 2009 to attend the meeting "TRP channels: from sensory signaling to human disease" and enjoyed an international, intensive and vibrant meeting. This meeting shed lights on the recent advances made in this field of research in different sectors of biology, and identified directions for future research and the areas where TRP channels could be used as potential targets for prevention and treatment of human diseases. The participants of this meeting shared their recent largely unpublished data, state-of-the-art techniques and their critical views which would push research in this field forward in the new decade. Another major outcome of this meeting was the realization that extensive work remains to be done to develop the necessary tools and enhance the quality of research in this area so that the prevailing controversies can be resolved. In this report we summarize the latest scientific excitements, some critical issues, as well as some future directions for research that were addressed and discussed in this meeting.
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| 4. |
- Gustafsson, Amanda Jabin, et al.
(author)
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ADP ribose is an endogenous ligand for the purinergic P2Y1 receptor
- 2011
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In: Molecular and Cellular Endocrinology. - : Elsevier BV. - 0303-7207 .- 1872-8057. ; 333:1, s. 8-19
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Journal article (peer-reviewed)abstract
- The mechanism by which extracellular ADP ribose (ADPr) increases intracellular free Ca2+ concentration ([Ca2+](i)) remains unknown. We measured [Ca2+](i) changes in fura-2 loaded rat insulinoma INS-1E cells, and in primary beta-cells from rat and human. A phosphonate analogue of ADPr (PADPr) and 8-Bromo-ADPr (8Br-ADPr) were synthesized. ADPr increased [Ca2+](i) in the form of a peak followed by a plateau dependent on extracellular Ca2+. NAD(+), cADPr, PADPr, 8Br-ADPr or breakdown products of ADPr did not increase [Ca2+](i). The ADPr-induced [Ca2+](i) increase was not affected by inhibitors of TRPM2, but was abolished by thapsigargin and inhibited when phospholipase C and IP3 receptors were inhibited. MRS 2179 and MRS 2279, specific inhibitors of the purinergic receptor P2Y1, completely blocked the ADPrinduced [Ca2+](i) increase. ADPr increased [Ca2+](i) in transfected human astrocytoma cells (1321N1) that express human P2Y1 receptors, but not in untransfected astrocytoma cells. We conclude that ADPr is a specific agonist of P2Y1 receptors.
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| 5. |
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| 6. |
- Islam, M. Shahidul, et al.
(author)
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Is road-side fishpond water in Bangladesh safe for human use? An assessment using water quality indices
- 2022
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In: Environmental Challenges. - : Elsevier BV. - 2667-0100. ; 6
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Journal article (peer-reviewed)abstract
- Pond water is used in everyday life by many people in Bangladesh, however, without sufficient and reliable information regarding water quality and pollution status. For this reason, geospatial analysis and mapping of water quality indices such as metal (MI), contamination (Cd), and physicochemical water quality index (WQI) were assessed to improve the understanding of potential pollution sources. Samples were collected from twenty randomly selected ponds situated in Jashore Sadar Upazila, Bangladesh. Nineteen (19) water quality parameters were measured, including pH, temperature, EC, TDS, total suspended solids (TSS), chloride, alkalinity, total hardness, salinity, Fe, Mn, Pb, Cr, Cd, Co, Zn, Ag, Ni, and Cu. The average concentration of Fe, Mn, Pb, Cd, and Ag was much higher than recommended standards. The WQI ranged from 1.59-5.27, Cd from -0.19-18.28, and MI from 7.81-26.28. The spatial distribution of MI indicates that the south-western and south-eastern region of the study area are stands out with a very high pollution pressure. The spatial distribution of Cd, follows the same trend as for MI. A multitude of different types of pollution sources contributes to the high pollution load such as, municipal wastewater, leachate from landfills, small industry wastewater and stormwater, and agricultural runoff. The studied pond water is highly polluted and not suitable for household use and fish consumption.
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| 7. |
- Islam, M. Shahidul, et al.
(author)
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Spatial Distribution and Source Identification of Water Quality Parameters of an Industrial Seaport Riverbank Area in Bangladesh
- 2022
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In: Water (Switzerland). - : MDPI AG. - 2073-4441. ; 14:9
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Journal article (peer-reviewed)abstract
- The Pasur River is a vital reservoir of surface water in the Sundarbon area in Bangladesh. Mongla seaport is located on the bank of this river. Many industries and other commercial sectors situated in this port area are discharging waste into the river without proper treatment. For this reason, geospatial analysis and mapping of water pollutant distribution were performed to assess the physicochemical and toxicological situation in the study area. We used different water quality indices such as Metal Index (MI), Comprehensive Pollution Index (CPI), and Weighted Arithmetic Water Quality Index Method (WQI) to improve the understanding of pollution distribution and processes determining the quality of river water. Multivariate statistical methods were used to evaluate loads and sources of pollutants in the Pasur River system. The results indicate that the sources of contaminants are both geogenic and anthropogenic, including untreated or poorly treated wastewater from industries and urban domestic waste discharge. The concentration range of total suspended solid (TSS), chloride, iron (Fe), and manganese (Mn) were from 363.2 to 1482.7, 108.2 to 708.93, 1.13 to 2.75, and 0.19 to 1.41 mg/L, respectively, significantly exceeding the health-based guideline of WHO and Bangladeshi standards. The high Fe and Mn contents are contributions from geogenic and anthropogenic sources such as industrial waste and construction activities. The average pH value was 8.73, higher than the WHO and Bangladeshi standard limit. WQI (ranging from 391 to 1336), CPI (6.71 to 23.1), and MI (7.23 to 23.3) were very high and greatly exceeded standard limits indicating that the Pasur River water is highly polluted. The results of this study can be used as a first reference work for developing a surface water quality monitoring system and guide decisionmakers for priorities regarding wastewater treatment.
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| 8. |
- Islam, M. Shahidul, et al.
(author)
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Toxicity and source identification of pollutants in an urban river in Bangladesh
- 2023
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In: Environmental Earth Sciences. - : Springer Science and Business Media LLC. - 1866-6280 .- 1866-6299. ; 82:6
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Journal article (peer-reviewed)abstract
- Urban rivers in Asian developing countries are becoming increasingly polluted due to industrialization and lacking treatment of wastewater. We investigated toxicity and likely sources of pollutants for the urban Shitalakshaya River, Bangladesh. Physiochemical variables and heavy metals were examined in water and sediment of an urban river section in Narayanganj City. The spatial distribution of quality indices and cluster groups indicates that the river’s downstream urban-affected areas are the most contaminated. Water and sediment quality guidelines indicate that COD, TSS, Fe, Pb, Zn in water, and Pb, Co, Ni, Cu, Zn, and particularly Pb and Cu in sediment, pose a serious threat to the aquatic ecosystem and human health in the area. Correlation, principal component (PCA), and cluster analysis (CA) indicate that the sources of Mn and Cd are geogenic, COD, TSS, Pb, Zn, Cu anthropogenic, and Fe, Ni, Co both geogenic and anthropogenic. The main anthropogenic pollution sources of the study area are municipal and industrial wastewater, boat and car traffic, runoff from agricultural areas, and stormwater runoff.
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| 9. |
- Krishnan, Kalaiselvan, et al.
(author)
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Calcium Signaling in a Genetically Engineered Human Pancreatic beta-Cell Line
- 2015
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In: Pancreas. - 0885-3177 .- 1536-4828. ; 44:5, s. 773-777
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Journal article (peer-reviewed)abstract
- Objectives The use of primary human -cells for studying Ca2+ signaling is limited by the scarcity of human pancreatic islets. Rodent insulinoma cell lines are widely used, but it is difficult to extrapolate results obtained from rodent cells to human. Recently, a genetically engineered human -cell line EndoC-BH1 has been developed. We have examined whether the EndoC-BH1 cells could be used as a model for studying Ca2+ signaling in the -cells. Methods We used microscope-based fluorometry to measure cytoplasmic-free Ca2+ concentration from fura-2-loaded single EndoC-BH1 cells cultured on glass cover slips. Ca2+ responses to different agonists of insulin secretion were studied. Insulin secretion was measured by radioimmunoassay. Results EndoC-BH1 cells secreted insulin in response to glucose in a dose-dependent manner, and the secretion was enhanced by GLP-1 (glucagon-like peptide 1). Glucose, potassium chloride, carbachol, l-arginine, and tolbutamide increased cytoplasmic-free Ca2+ concentration in the EndoC-BH1 cells. We found that GLP-1 was essential for Ca2+ response to glucose and tolbutamide. Conclusions We concluded that the EndoC-BH1 cells can be used as model cells to study Ca2+ signaling and stimulus-secretion coupling in the human -cells.
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| 10. |
- Krishnan, Kalaiselvan, et al.
(author)
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Calcium signaling in a genetically engineered human pancreatic β-cell line
- 2015
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In: Pancreas. - 0885-3177 .- 1536-4828. ; 44:5, s. 773-777
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Journal article (peer-reviewed)abstract
- Objectives: The use of primary human β-cells for studying Ca2+ signalingis limited by the scarcity of human pancreatic islets. Rodent insulinomacell lines are widely used, but it is difficult to extrapolate results obtainedfrom rodent cells to human. Recently, a genetically engineered humanβ-cell line EndoC-BH1 has been developed. We have examined whetherthe EndoC-BH1 cells could be used as a model for studying Ca2+ signalingin the β-cells.Methods: We used microscope-based fluorometry to measure cytoplasmicfreeCa2+ concentration from fura-2–loaded single EndoC-BH1 cellscultured on glass cover slips. Ca2+ responses to different agonists of insulinsecretion were studied. Insulin secretion was measured by radioimmunoassay.Results: EndoC-BH1 cells secreted insulin in response to glucose ina dose-dependent manner, and the secretion was enhanced by GLP-1(glucagon-like peptide 1). Glucose, potassium chloride, carbachol, L-arginine,and tolbutamide increased cytoplasmic-free Ca2+ concentration in theEndoC-BH1 cells. We found that GLP-1 was essential for Ca2+ responseto glucose and tolbutamide.Conclusions: We concluded that the EndoC-BH1 cells can be used asmodel cells to study Ca2+ signaling and stimulus-secretion coupling inthe human β-cells.
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