| 1. |
- Georgievska, Biljana, et al.
(author)
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Regulated delivery of glial cell line-derived neurotrophic factor into rat striatum, using a tetracycline-dependent lentiviral vector.
- 2004
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In: Human Gene Therapy. - : Mary Ann Liebert Inc. - 1043-0342 .- 1557-7422. ; 15:10, s. 934-944
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Journal article (peer-reviewed)abstract
- In this study, a tetracycline-regulated lentiviral vector system, based on the tetracycline-dependent transactivator rtTA2S-M2, was developed for controlled expression of glial cell line-derived neurotrophic factor (GDNF) in the rat brain. Expression of the marker gene green fluorescent protein (GFP) and GDNF was tightly regulated in a dose-dependent manner in neural cell lines in vitro. Injection of high-titer lentiviral vectors into the rat striatum resulted in a 7-fold induction of GDNF tissue levels (1060 pg/mg tissue), when doxycycline (a tetracycline analog) was added to the drinking water. However, low levels of GDNF (150 pg/mg tissue) were also detected in animals that did not receive doxycycline, indicating a significant background leakage from the vector system in vivo. The level of basal expression was markedly reduced when a 10-fold lower dose of the tetracycline-regulated GDNF vector was injected into the striatum (3–11 pg/mg tissue), and doxycycline- induced GDNF tissue levels obtained in these animals were about 190 pg/mg tissue. Doxycycline-induced expression of GDNF resulted in a significant downregulation of the tyrosine hydroxylase (TH) protein in the intact striatum. Removal of doxycycline from the drinking water rapidly (within 3 days) turned off transgenic GDNF mRNA expression and GDNF protein levels in the tissue were completely reduced by 2 weeks, demonstrating the dynamics of the system in vivo. Accordingly, TH protein expression returned to normal by 2–8 weeks after removal of doxycycline, indicating that GDNF-induced downregulation of TH is a reversible event.
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| 2. |
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| 3. |
- Jakobsson, Johan, et al.
(author)
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Lentiviral vectors.
- 2003
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In: International Review of Neurobiology. - 0074-7742. ; 55, s. 111-122
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Research review (peer-reviewed)
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| 4. |
- Jakobsson, Johan, et al.
(author)
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Lesion-dependent regulation of transgene expression in the rat brain using a human glial fibrillary acidic protein-lentiviral vector.
- 2004
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In: European Journal of Neuroscience. - 1460-9568. ; 19:3, s. 761-765
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Journal article (peer-reviewed)abstract
- The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. The most commonly used systems are based on a transactivator in combination with a drug, e.g. the tetracycline-regulated system. Here we describe a different method of transgene regulation by the use of the human glial fibrillary acidic protein (GFAP) promoter. We constructed a lentiviral vector that directs transgene expression to astrocytes. Using toxin-induced lesions we investigated to what extent transgene expression could be regulated in accordance with the activation of the endogenous GFAP gene. In animals receiving excitotoxic lesions of the striatum we detected an eightfold increase of green fluorescent protein (GFP)-expressing cells. The vast majority of these cells did not divide, suggesting that the transgene was indeed regulated in a similar fashion as the endogenous GFAP gene. This finding will lead to the development of lentiviral vectors with autoregulatory capacities that may be very useful for gene therapy to the brain.
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| 5. |
- Jakobsson, Johan, et al.
(author)
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Targeted transgene expression in rat brain using lentiviral vectors.
- 2003
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In: Journal of Neuroscience Research. - : Wiley. - 1097-4547 .- 0360-4012. ; 73:6, s. 876-885
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Journal article (peer-reviewed)abstract
- Direct gene transfer to the adult brain is dependent on vectors that transduce non-dividing cells, such as lentiviral vectors. Another aspect of the development of gene therapy to the brain is the need for cell-specific transgene expression. Expression from vesicular stomatitis virus G-protein (VSV-G) pseudotyped lentiviral vectors has been reported to be mainly neuron specific in the brain. We constructed cell-specific lentiviral vectors using the neuron-specific enolase (rNSE) or the glial fibrillary acidic protein (hGFAP) promoters and compared them to the ubiquitous human cytomegalovirus promoter (hCMV), a hybrid CMV/-actin promoter (CAG) and the promoter for human elongation factor 1 (EF1). Our results showed that the hGFAP promoter was expressed only in glial cells, whereas rNSE was purely neuron specific, showing that VSV-G is pantropic in the rat striatum. We conclude that the VSV-G allows transduction of both glial and neuronal cells and the promoter dictates in what cell type the transgene will be expressed. The expression of transgenes exclusively in astrocytes would allow for local delivery of secreted transgene products, such as glial cell line-derived neurotrophic factor (GDNF), circumventing the anterograde transport that may induce unwanted side effects.
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| 6. |
- Modig, Niclas, 1973-
(author)
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Hur kan ekonomi göras begripligt? : En studie om villkor för kraftfull ekonomiundervisning i samhällskunskap
- 2023
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Doctoral thesis (other academic/artistic)abstract
- This thesis seeks to understand what constitutes important economics knowledge from a disciplinary perspective and how such knowledge is recontextualized in textbooks and teacher training to become teachable to social studies students in Sweden’s upper secondary schools. It consists of four substudies: two covering disciplinary economics knowledge and two covering preservice social studies teachers. The first and second substudies focus on disciplinary economics knowledge. The first substudy, which was based on an online questionnaire for Swedish economics scholars at higher education institutions, shows that six economics terms/principles are especially important for people to understand. Such knowledge may be considered powerful economics knowledge. The second substudy demonstrates that there are great variations in the extent to which powerful economics terms appear in Swedish social studies textbooks and in how the language used shifts between everyday and scientific language. There is a risk that not all learners are given equal preconditions to develop economics knowledge through social studies textbooks. The third and fourth substudies focus on how preservice social studies teachers develop the ability to teach economic knowledge and show that students’ individual pedagogical content knowledge (PCK) depends on theoretical and practical studies. Students are unconfident about their economics knowledge, which may negatively affect their willingness and ability to teach economics when they become in-service social studies teachers.Overall, there appears to be a problem with the recontextualization of economics knowledge in Swedish social studies textbooks and with economics education for preservice social studies teachers. This may negatively affect economics education. As a result, it is important to strengthen preservice social studies teachers’ economics knowledge through increased economics content during teacher training. The importance of imparting powerful economics knowledge and shifting between everyday and scientific language in textbooks and during teaching needs to be highlighted for policymakers, textbook authors, and teacher educators.
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| 7. |
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| 8. |
- Boström, Jannika, et al.
(author)
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Autumn migratory fuelling : a response to simulated magnetic displacement in juvenile wheatears, Oenathe oenathe
- 2010
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In: Behavioral Ecology and Sociobiology. - : Springer Science and Business Media LLC. - 0340-5443 .- 1432-0762. ; 64:11, s. 1725-1732
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Journal article (peer-reviewed)abstract
- Recent experiments exposing migratory birds to altered magnetic fields simulating geographical displacements have shown that the geomagnetic field acts as an external cue affecting migratory fuelling behaviour. This is the first study investigating fuel deposition in relation to geomagnetic cues in long-distance migrants using the western passage of the Mediterranean region. Juvenile wheatears (Oenanthe oenanthe) were exposed to a magnetically simulated autumn migration from southern Sweden to West Africa. Birds displaced parallel to the west of their natural migration route, simulating an unnatural flight over the Atlantic Ocean, increased their fuel deposition compared to birds experiencing a simulated migration along the natural route. These birds, on the other hand, showed relatively low fuel loads in agreement with earlier data on wheatears trapped during stopover. The experimental displacement to the west, corresponding to novel sites in the Atlantic Ocean, led to a simulated longer distance to the wintering area, probably explaining the observed larger fuel loads. Our data verify previous results suggesting that migratory birds use geomagnetic cues for fuelling decisions and, for the first time, show that birds, on their first migration, can use geomagnetic cues to compensate for a displacement outside their normal migratory route, by adjusting fuel deposition.
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| 9. |
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| 10. |
- Chard, Emelie, et al.
(author)
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Gender and cross-country differences in the determinants of sustainable diet intentions: a multigroup analysis of the UK, China, Sweden, and Brazil
- 2024
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In: FRONTIERS IN PSYCHOLOGY. - 1664-1078. ; 15
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Journal article (peer-reviewed)abstract
- Introduction Gender differences have been identified in both the engagement in and the determinants of sustainable diet behaviours. However, as engagement in pro-environmental behaviours varies across countries, the consistency of gender differences could follow similar patterns. Understanding the factors underlying gender and country differences in diet intentions is important for determining how to promote sustainable diets in different populations.Methods Using survey data from the UK, China, Sweden and Brazil (N=4,569), this paper examines the influence of subjective norms, diet-related identity, perceived status of meat consumption, environmental identity and environmental concern on sustainable diet intentions. Multigroup analysis was used to explore gender and country differences in the influence of these variables, and whether gender differences were consistent across the four countries.Results The findings show that there are gender differences in the influence of diet-related identity and perceived status of meat consumption, as well as cross-country differences in all factors except subjective norms. Holding a strong diet-related meat identity negatively affected sustainable diet intentions in all groups. Crucially, however, gender differences are not consistent across countries.Discussion These results suggest that individuals' intentions to engage in sustainable diet behaviours are influenced by nationally unique gender associations.
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