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- Hareza, Agnieszka, et al.
(author)
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Phosphoproteomic insights into processes influenced by the kinase-like protein DIA1/C3orf58
- 2018
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In: PeerJ. - : PeerJ. - 2167-8359. ; 2018:4
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Journal article (peer-reviewed)abstract
- Many kinases are still 'orphans,' which means knowledge about their substrates, and often also about the processes they regulate, is lacking. Here, DIA1/C3orf58, a member of a novel predicted kinase-like family, is shown to be present in the endoplasmic reticulum and to influence trafficking via the secretory pathway. Subsequently, DIA1 is subjected to phosphoproteomics analysis to cast light on its signalling pathways. A liquid chromatography-tandem mass spectrometry proteomic approach with phosphopeptide enrichment is applied to membrane fractions of DIA1-overexpressing and control HEK293T cells, and phosphosites dependent on the presence of DIA1 are elucidated. Most of these phosphosites belonged to CK2- and proline-directed kinase types. In parallel, the proteomics of proteins immunoprecipitated with DIA1 reported its probable interactors. This pilot study provides the basis for deeper studies of DIA1 signalling.
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- Hilszczanski, Jacek, et al.
(author)
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Surrogate tree cavities : boxes with artificial substrate can serve as temporary habitat for Osmoderma barnabita (Motsch.) (Coleoptera, Cetoniinae)
- 2014
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In: Journal of Insect Conservation. - : Springer. - 1366-638X .- 1572-9753. ; 18:5, s. 855-861
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Journal article (peer-reviewed)abstract
- Many saproxylic insects have declined or became extinct, mainly due to habitat loss and fragmentation, and their survival increasingly depends on active conservation. Efforts to achieve this goal may be supported by the introduction of new methods, including creation of artificial habitats. Here we present results of studies on the use of wooden boxes mimicking tree cavities for an endangered saproxylic species, Osmoderma barnabita. Boxes were filled with the feeding substrate for larvae and installed on trees. Second and third-instar O. barnabita larvae were introduced in half of the boxes; the remaining ones were left uninhabited. Later inspection of boxes showed a high survival rate of introduced larvae, as well as successful breeding of a new generation inside the boxes. At the same time boxes were not colonized by the local population of O. barnabita, although other cetoniids did so. The co-occurring larvae of other cetoniids did not affect O. barnabita larvae. Thermal conditions inside boxes and natural tree cavities were almost identical and based on the results of our studies we conclude that wooden boxes may serve as temporary habitat for O. barnabita. They may be particularly useful in cases of destruction of species natural habitat, in restoration programs, and have the potential to act as a stepping stones in cases of a lack of habitat continuity.
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- Jaworski, Jacek, et al.
(author)
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Sources of breathing pattern variability in the respiratory feedback control loop
- 2019
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In: Journal of Theoretical Biology. - : Academic Press. - 0022-5193 .- 1095-8541. ; 469, s. 148-162
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Journal article (peer-reviewed)abstract
- The variability of the breath-to-breath breathing pattern, and its alterations in disease, may hold information of physiologic and/or diagnostic value. We hypothesized that this variability arises from the way that noise is processed within the respiratory feedback control loop, and that pathologic alterations to specific components within the system give rise to characteristic alterations in breathing pattern variability. We explored this hypothesis using a computational model of the respiratory control system that integrates mechanical factors, gas exchange processes, and chemoreceptor signals to simulate breathing patterns subject to the influences of random variability in each of the system components. We found that the greatest changes in the coefficient of variation (CV) of both breathing amplitude and timing were caused by increases in lung resistance and impairments in gas exchange, both common features of pulmonary disease. This suggests that breathing pattern variability may reflect discernible deterministic processes involved in the control of breathing. © 2019 Elsevier Ltd
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- Landtblom, Anne-Marie, et al.
(author)
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Absolute metabolite concentrations in cerebral white matter of multiple sclerosis patients with beta interferon treatment
- 2008
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In: Multiple Sclerosis Journal. - : Sage Publications. - 1352-4585 .- 1477-0970. ; 14:Suppl. 1, s. S162-S162
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Journal article (peer-reviewed)abstract
- Background: A few investigations concern interferon (IFN)-treated multiple sclerosis (MS) patients using proton spectroscopy, however not with an absolute quantitation or during extended treatment.Objective: To quantify metabolite changes during IFN therapy using magnetic resonance spectroscopy.Methods: We included 14 MS patients, (9 men, 5 women, mean age 41.8 years, mean disease duration 10.9 years, 9 with relapsing-remitting MS, 6 with secondary progressive and bouts) scheduled for immunomodulatory treatment (5 IFN1A, 9 IFN1B) as well as 14 healthy controls, (8 men, 6 women, mean age 40.2 years). All patients had clinically definite MS (Poser criteria). Measurements were performed in white matter (four voxels).Results: Longitudinal results: N-acetylaspartate + Nacetylapartylglutamate (NAA+NAAG) showed a trend to higher values before treatment. Myo-inositol concentrations were significantly and increasingly elevated (p=0.03). Glutamine and glutamate concentrations dropped significantly (p=0.009) after treatment started but raised later. MS patients/ healthy controls: Creatine and myo-inositol concentrations were significantly higher in MS patients before and after treatment. NAA + NAAG concentrations were significantly lower before and after treatment. Glutamine and glutamate concentrations were higher before therapy, later equal to healthy controls.Conclusions: IFN-treated patients demonstrate increasing myoinositol, a marker of progressive glial proliferation. Also, decreasing concentrations of total NAA derivatives despite IFN therapy suggest ongoing progressive pathology and constant neuronal loss in the course of MS. MS patients compared with matched healthy controls show highly significant differences regarding metabolites (Cr, myo- Ins, NAA) that increase during the therapy period, also indicating that the medication can only moderately influence the metabolites. The most interesting finding is related the excitatory molecules, glutamine and glutamate. Before IFN therapy statistical analysis showed significant elevation in the concentrations; however after IFN therapy this difference is no longer observed. This finding underlines a possible role of IFN in the expression of down-regulating excitotoxic molecules.
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