| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Gao, Hongkai, et al.
(författare)
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Stepwise modeling and the importance of internal variables validation to test model realism in a data scarce glacier basin
- 2020
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Ingår i: Journal of Hydrology. - : Elsevier BV. - 0022-1694. ; 591
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Tidskriftsartikel (refereegranskat)abstract
- Model realism is of vital importance in science of hydrology, in terms of realistic representation of hydrological processes and reliability of future prediction. Here, we employed a stepwise modeling approach that leverages flexible model structures and multi-source observations for robust streamflow simulation and internal variables validation with improved model realism. This framework is demonstrated in Yigong Zangbu River (YZR) basin, a data scarce glacier basin in the upper Brahmaputra River. We designed six experiments (Exp1–6) to use modeling as a tool to understand hydrological processes in this remote cold basin with extremely high altitude. In Exp1, we started with a distributed rainfall-runoff model (FLEXD) - representing the case that snow and glacier processes were ignored. Then, we stepwisely added snow and glacier processes into FLEXD, denoted as FLEXD-S (Exp2) and FLEXD-SG (Exp3), respectively, and such improvement of model structure led to significantly improved streamflow estimates. To explore the impact of different precipitation forcing on model performance, FLEXD-SG was driven by Theissen average (Exp3) and three individual stations’ precipitation (Exp4–6). The model realism was tested by observed hydrograph, snow cover area (SCA) and glacier mass balance (GMB). Results showed that a robust and realistic hydrological modeling system was achieved in Exp6. In this modeling study, we learned that: 1) stepwise modeling is effective in investigating catchment behavior, and snow and glacier melting are the dominant hydrological processes in the YZR basin; 2) internal variables validation is beneficial to test model realism in data scarce basin; 3) the FLEXD-SG model calibrated by only one year hydrograph is sufficient to reproduce snow and glacier variations; 4) precipitation of a single station as forcing data could outperform Theissen average; 5) based on the well tested model configuration in Exp6, we analyzed simulated results, and reconstructed the long term hydrography (1961–2013), to support the potential competence for decision making on water resources management in practice. The proposed framework may significantly improve our skills in hydrological modeling over data-poor regions.
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| 3. |
- Hou, Meijun, et al.
(författare)
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Human dopaminergic system in the exercise-cognition link
- 2024
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Ingår i: Trends in Molecular Medicine. - : Elsevier. - 1471-4914 .- 1471-499X.
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Tidskriftsartikel (refereegranskat)abstract
- While the dopaminergic system is important for cognitive processes, it is also sensitive to the influence of physical activity (PA). We summarize current evidence on whether PA-related changes in the human dopaminergic system are associated with alterations in cognitive performance, discuss recent advances, and highlight challenges and opportunities for future research.
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| 4. |
- Kristanl, Matej, et al.
(författare)
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The Seventh Visual Object Tracking VOT2019 Challenge Results
- 2019
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Ingår i: 2019 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW). - : IEEE COMPUTER SOC. - 9781728150239 ; , s. 2206-2241
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2019 is the seventh annual tracker benchmarking activity organized by the VOT initiative. Results of 81 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis as well as the standard VOT methodology for long-term tracking analysis. The VOT2019 challenge was composed of five challenges focusing on different tracking domains: (i) VOT-ST2019 challenge focused on short-term tracking in RGB, (ii) VOT-RT2019 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2019 focused on long-term tracking namely coping with target disappearance and reappearance. Two new challenges have been introduced: (iv) VOT-RGBT2019 challenge focused on short-term tracking in RGB and thermal imagery and (v) VOT-RGBD2019 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2019, VOT-RT2019 and VOT-LT2019 datasets were refreshed while new datasets were introduced for VOT-RGBT2019 and VOT-RGBD2019. The VOT toolkit has been updated to support both standard short-term, long-term tracking and tracking with multi-channel imagery. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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| 5. |
- Liu, Yi, et al.
(författare)
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Micro-hydrothermal reactions mediated grain growth during spark plasma sintering of a carbonate-containing hydroxyapatite nanopowder
- 2014
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Ingår i: Journal of the European Ceramic Society. - : Elsevier BV. - 0955-2219 .- 1873-619X. ; 34:16, s. 4395-4401
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Tidskriftsartikel (refereegranskat)abstract
- A carbonate-containing hydroxyapatite nanopowder was consolidated by spark plasma sintering at the temperatures ranging from 650 to 1100 degrees C. It was found that the water released by dehydroxylation was trapped inside the nanopores in the densified HAp bodies over 900 degrees C. Based on the analysis by the X-ray diffraction, Fourier-transform infrared spectrometry and scanning electron microscope, the water-nanopore system was evaluated and its effect on the grain growth was also investigated. It was revealed that the water existed inside the closed nanopores most probably resulted in the formation of local micro-hydrothermal environments inside bulk HAp ceramics during SPS. Therefore, the grain growth was enhanced by the local micro-hydrothermal reactions activated above 900 degrees C. In addition, abnormal grain growth was also observed when a higher temperature or higher heating rate was employed, which may be attributed to the local highly active hydrothermal reactions.
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| 6. |
- Liu, Yi, et al.
(författare)
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Reactive consolidation of layered-ternary Ti(2)AlN ceramics by spark plasma sintering of a Ti/AlN powder mixture
- 2011
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Ingår i: Journal of the European Ceramic Society. - : Elsevier BV. - 0955-2219 .- 1873-619X. ; 31:5, s. 863-868
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Tidskriftsartikel (refereegranskat)abstract
- A reactive consolidation process for preparing ternary Ti(2)AlN ceramics was investigated by spark plasma sintering (SPS). A Ti/AlN powder mixture with a molar ratio of 2:1 was consolidated at temperatures ranging from 800 to 1450 degrees C. The phase composition and microstructure evolution during the process were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) equipped with an energy dispersive spectroscopy (EDS). A series of intermediate phases, namely TiN, Ti(3)Al, Ti(3)AlN and TiAl were indentified, which revealed a reaction pathway towards the formation of Ti(2)AlN.
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