| 1. |
- Eklöf, Ingemar, et al.
(author)
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Askor för konstruktionsändamål : slutrapport 2016-03-15
- 2016
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Reports (other academic/artistic)abstract
- Vid förbränning av biobränslen och avfall genereras restprodukter, så kallade energiaskor, som kan användas som konstruktionsmaterial. I detta notat 8-2016 redovisas ett projekt som identifierat viktiga åtgärder i syfte att öka användningen av energiaskor för konstruktionsändamål. En stor andel av engergiaskorna har, i ursprunglig form eller efter sortering och behandling, goda konstruktionsmässiga egenskaper. För närvarande används energiaskor i Sverige till största delen som konstruktionsmaterial inom avfallsanläggningar; främst för sluttäckning av äldre avfallsdeponier. Men sluttäckningarna avslutas nu fortlöpande och behovet når sin kulmen inom de närmaste 5–6 åren. Därför behövs nya användningsområden för energiaskor, annars kommer askorna att deponeras i allt större utsträckning vilket är en dålig hushållning av resurser.Genom att använda energiaskor för konstruktioner, i exempelvis vägar, parkeringsplatser och för andra anläggningsändamål, kan man ersätta jungfruliga råvaror vilket gör energi- och avfallssystemet mer hållbart. Om inte andra avsättningsalternativ utvecklas ökar dessutom kostnaderna för askhanteringen, vilket belastar energiproducenterna och i slutändan också slutkonsumenterna.
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| 2. |
- Evander, Mikael, et al.
(author)
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Acoustic trapping of cells in a microfluidic format
- 2005
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In: Proceedings of µTAS 2005 Conference. ; 1, s. 515-517
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Conference paper (peer-reviewed)abstract
- This paper presents, for the first time, non-contact acoustic trapping of cells in a microfluidic format. The employed acoustic force maintains the cells in the center of a fluidic channel while allowing for perfusion of e.g. nutrients or drugs as well as optical monitoring of the cells. Neural stem cells have been acoustically trapped and tested for viability after 15 minutes of ultrasonic radiation. It is also shown that it is possible to grow yeast cells suspended in an acoustic standing wave while perfusing with cell media.
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| 3. |
- Evander, Mikael, et al.
(author)
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Acoustic Trapping: System Design, Optimization and Applications
- 2006
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In: Proceedings of the sixth Micro Structure Workshop. ; 1, s. 33-33
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Conference paper (peer-reviewed)abstract
- Manipulation, separation and trapping of particles and cells are very important tools in today's bioanalytical and medical field. The acoustic no-contact trapping method presented at earlier MSW 2004 provides a flexible platform for performing cell and particle assays in a perfusion-based microsystem. To further develop the system microfabricated glass channels are now used, resulting in shorter fabrication times and a very inert channel material. The fluidic design has been revised to minimise the risks of leaking and hydrodynamic focusing has been incorporated to ensure a high trapping efficiency. A change of piezoelectric materials has resulted in less thermal losses in the material, higher reproducibility and shorter manufacturing time. The trapping force was estimated by calculating the fluid force exerted on a single particle levitated in the standing wave as a reference. The temperature increase due to the losses in the transducer was measured using a fluorescent dye, indicating a maximum temperature increase of 10 degrees Celsius. Live cells have been trapped and shown to be viable while still suspended in the standing wave, thus making it possible to do on-line studies on, for example, drug response of cell populations.
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| 4. |
- Evander, Mikael, et al.
(author)
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Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays
- 2007
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In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 79:7, s. 2984-2991
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Journal article (peer-reviewed)abstract
- Techniques for manipulating, separating, and trapping particles and cells are highly desired in today's bioanalytical and biomedical field. The microfluidic chip-based acoustic noncontact trapping method earlier developed within the group now provides a flexible platform for performing cell- and particle-based assays in continuous flow microsystems. An acoustic standing wave is generated in etched glass channels (600x61 microm2) by miniature ultrasonic transducers (550x550x200 microm3). Particles or cells passing the transducer will be retained and levitated in the center of the channel without any contact with the channel walls. The maximum trapping force was calculated to be 430+/-135 pN by measuring the drag force exerted on a single particle levitated in the standing wave. The temperature increase in the channel was characterized by fluorescence measurements using rhodamine B, and levels of moderate temperature increase were noted. Neural stem cells were acoustically trapped and shown to be viable after 15 min. Further evidence of the mild cell handling conditions was demonstrated as yeast cells were successfully cultured for 6 h in the acoustic trap while being perfused by the cell medium at a flowrate of 1 microL/min. The acoustic microchip method facilitates trapping of single cells as well as larger cell clusters. The noncontact mode of cell handling is especially important when studies on nonadherent cells are performed, e.g., stem cells, yeast cells, or blood cells, as mechanical stress and surface interaction are minimized. The demonstrated acoustic trapping of cells and particles enables cell- or particle-based bioassays to be performed in a continuous flow format.
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| 5. |
- Evander, Mikael, et al.
(author)
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Versatile microchip utilising ultrasonic standing waves
- 2005
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In: IFMBE Proceedings 2005. ; , s. 123-124
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Conference paper (peer-reviewed)abstract
- This paper presents the concept and initial work on a microfluidic platform for bead-based analysis of biological sample. The core technology in this project is ultrasonic manipulation and trapping of particle in array configurations by means of acoustic forces. The platform is ultimately aimed for parallel multistep bioassays performed on biochemically activated microbeads (or particles) using submicrolitre sample volumes. A first prototype with three individually controlled particle trapping sites has been developed and evaluated. Standing ultrasonic waves were generated across a microfluidic channel by integrated PZT ultrasonic microtransducers. Particles in a fluid passing a transducer were drawn to pressure minima in the acoustic field, thereby being trapped and confined laterally over the transducer. It is anticipated that acoustic trapping using integrated transducers can be exploited in miniaturised total chemical analysis systems (µTAS), where e.g. microbeads with immobilised antibodies can be trapped in arrays and subjected to minute amounts of sample followed by a reaction, detected using fluorescence. Preliminary results indicate that the platform is capable of handling live cells as well as microbeads. A first model bioassay with detection of fluorescein marked avidin binding to trapped biotin beads has been evaluated.
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| 6. |
- Hung, Rayjean J., et al.
(author)
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Lung Cancer Risk in Never-Smokers of European Descent is Associated With Genetic Variation in the 5(p)15.33 TERT-CLPTM1Ll Region
- 2019
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In: Journal of Thoracic Oncology. - : ELSEVIER SCIENCE INC. - 1556-0864 .- 1556-1380. ; 14:8, s. 1360-1369
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Journal article (peer-reviewed)abstract
- Introduction: Inherited susceptibility to lung cancer risk in never-smokers is poorly understood. The major reason for this gap in knowledge is that this disease is relatively uncommon (except in Asians), making it difficult to assemble an adequate study sample. In this study we conducted a genome-wide association study on the largest, to date, set of European-descent never-smokers with lung cancer. Methods: We conducted a two-phase (discovery and replication) genome-wide association study in never-smokers of European descent. We further augmented the sample by performing a meta-analysis with never-smokers from the recent OncoArray study, which resulted in a total of 3636 cases and 6295 controls. We also compare our findings with those in smokers with lung cancer. Results: We detected three genome-wide statistically significant single nucleotide polymorphisms rs31490 (odds ratio [OR]: 0.769, 95% confidence interval [CI]: 0.722-0.820; p value 5.31 x 10(-16)), rs380286 (OR: 0.770, 95% CI: 0.723-0.820; p value 4.32 x 10(-16)), and rs4975616 OR: 0.778, 95% CI: 0.730-0.829; p value 1.04 x 10(-14)). All three mapped to Chromosome 5 CLPTM1L-TERT region, previously shown to be associated with lung cancer risk in smokers and in never-smoker Asian women, and risk of other cancers including breast, ovarian, colorectal, and prostate. Conclusions: We found that genetic susceptibility to lung cancer in never-smokers is associated to genetic variants with pan-cancer risk effects. The comparison with smokers shows that top variants previously shown to be associated with lung cancer risk only confer risk in the presence of tobacco exposure, underscoring the importance of gene-environment interactions in the etiology of this disease. (C) 2019 International Association for the Study of Lung Cancer. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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| 7. |
- Höckerdal, Karin, 1983-
(author)
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Enhancing the process of environmental improvement in manufacturing industry
- 2012
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Licentiate thesis (other academic/artistic)abstract
- To enable competitive industrial production, it is crucial to understand how to design and operate competitive production systems. Today, much operations management research focuses on the environmental dimension of sustainable manufacturing. Even though the environmental impact of industrial and commercial activities has become more vivid and severe, evidence points to a slow diffusion of industry-led ideas and actions for improving the environmental performance. It seems as if the manufacturing industry is still in the beginning of the process towards designing and operating environmentally benign production systems. However, increasing raw material and energy prices as well as potential penalties for lacking compliance provide cost related incentives for manufacturing companies to reduce their environmental impacts. Since production typically determines several environmental impacts such as exploitation of resources, contamination of soil, water and air, generation and emission of greenhouse gases etc., an important opportunity to reduce the environmental impact is found within a company’s production activities. In this, environmental issues can be viewed as either constraints or opportunities for competition. Companies can choose to adopt different positions ranging on a continuum from re-active behaviour, merely complying with environmental laws and regulations, to pro-active positions where competitive advantages are sought for. Either way, the increased environmental concern requires manufacturing companies to develop strategies, technologies and practices that will reduce the environmental impact from production.This research focuses on the process of environmental improvement applied by companies within the manufacturing industry and aims to explore the current state as well as develop support for enhancing this process. By collecting and analysing the empirical and theoretical findings, common barriers facing the companies in their strive to reduce the environmental impact from production as well as enabling factors, supporting the progress towards increased environmental performance have been identified. Based on an analysis of the current state one example of a link of challenges impeding the process of environmental improvement has been identified. The identified link of causes and effects shows a path towards a reactive improvement approach when dealing with environmental issues in production. In order to reach a more proactive state this link had to be broken. A general pattern describing the stages from a reactive to a proactive approach was identified, indicating that an increased understanding of and commitment to environmental issues were crucial parameters in order to succeed. Based on the findings, a number of requirements for enhancing the process of environmental improvement in production were derived. It was concluded that developing a support in terms of an improvement process was one way of improving the current state. Using an existing visual structure as a baseline a process for Green Performance Mapping was developed in cooperation with the studied companies. Ultimately, the suggested process was evaluated against the stated requirements and suggested as a support for enhancing the process of environmental improvement in production within the manufacturing industry, thereby contributing to reducing the environmental impact from production.
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| 8. |
- Johansson, Eva, et al.
(author)
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Evaluation of materials supply systems during product development projects
- 2004
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In: Proceedings of the Euroma Conference.
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Conference paper (other academic/artistic)abstract
- This paper discusses the process of designing materials supply systems during product development projects with focus on the evaluation part. A case study was conducted in close cooperation with a company within the automotive industry. Two performance measures for the materials supply systems evaluation were selected, namely inventory value and handling required for the materials supply. The result from the case study is an analysis of how these performance measures can be assessed early in product development projects and how they can be used in the process of materials supply systems design.
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| 9. |
- Johansson, Eva, et al.
(author)
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Evaluation of materials supply systems during product development projects
- 2006
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In: International Journal of Production Research. - : Informa UK Limited. - 0020-7543 .- 1366-588X. ; 44:5, s. 903-917
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Journal article (peer-reviewed)abstract
- The aim of this paper is to analyse how materials supply systems can be evaluated during product development projects in a concurrent engineering context. The focus is on performance assessment of materials supply systems and the input data required. A case study was conducted in close cooperation with a company and two performance measures were assessed during the concept definition phase and recalculated during the product and process validation phase. The results show that preliminary input data can be used to calculate performance measures by means of the following approaches: indicators, standards or scenarios. Furthermore, it is discussed how performance assessment can assist the materials supply systems design process in a concurrent engineering context by facilitating early problem discovery, early decision-making, and common understanding.
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| 10. |
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