| 1. |
- Ejlertsson, Jörgen, et al.
(author)
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Effects of co-disposal of wastes containing organic pollutants with municipal solid waste : a landfill simulation reactor study
- 2003
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In: Advances in Environmental Research. - 1093-0191 .- 1093-7927. ; 7:4, s. 949-960
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Journal article (peer-reviewed)abstract
- Different phases of the life cycle of a landfill receiving municipal solid waste (MSW) were monitored in landfill simulation reactors (LSRs) with the aim of investigating the effects of co-disposal of wastes containing organic pollutants (OPs) with MSW. Two LSRs out of four filled with well-characterised MSW received waste materials containing OPs. These included two types of plasticised PVC flooring materials, freon-blown insulation and phosphorus- and nitrogen-based flame-protected materials. Each of the two LSRs was operated under acid fermentative and neutral methanogenic conditions, respectively as were their corresponding controls, i.e. without extra OP. The methanogenic consortia degrading MSW were hampered by the addition of wastes containing OPs, probably due to the presence of Freon R11 and its degradation product, R21. The concentrations of R11 and R21 ranged between 0.1 and 1800 mg m super(-3) depending on the biogas production rate in the OP-amended LSRs. Losses of butylbenzyl- (26%) and bis(2-ethylhexyl)phthalate (15%) from one of two flooring materials was observed, whereas the other remained unaffected. Methanogenic conditions favoured the loss of plasticisers as compared to acidogenic conditions. Total phosphorus was significantly higher in the OP-spiked LSRs, which indicated a transformation of the non-halogenated flame-retardants.
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| 4. |
- Eriksson, D, et al.
(author)
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Extended exome sequencing identifies BACH2 as a novel major risk locus for Addison's disease
- 2016
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In: Journal of Internal Medicine. - : Wiley. - 0954-6820 .- 1365-2796. ; 286:6, s. 595-608
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Journal article (peer-reviewed)abstract
- BACKGROUND: Autoimmune disease is one of the leading causes of morbidity and mortality worldwide. In Addison's disease, the adrenal glands are targeted by destructive autoimmunity. Despite being the most common cause of primary adrenal failure, little is known about its aetiology.METHODS: To understand the genetic background of Addison's disease, we utilized the extensively characterized patients of the Swedish Addison Registry. We developed an extended exome capture array comprising a selected set of 1853 genes and their potential regulatory elements, for the purpose of sequencing 479 patients with Addison's disease and 1394 controls.RESULTS: We identified BACH2 (rs62408233-A, OR = 2.01 (1.71-2.37), P = 1.66 × 10(-15) , MAF 0.46/0.29 in cases/controls) as a novel gene associated with Addison's disease development. We also confirmed the previously known associations with the HLA complex.CONCLUSION: Whilst BACH2 has been previously reported to associate with organ-specific autoimmune diseases co-inherited with Addison's disease, we have identified BACH2 as a major risk locus in Addison's disease, independent of concomitant autoimmune diseases. Our results may enable future research towards preventive disease treatment.
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| 5. |
- Flowers, Sarah A., et al.
(author)
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Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking
- 2020
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In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 295:47, s. 16023-16036
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Journal article (peer-reviewed)abstract
- The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development. Lubricin's ability to reduce friction is related to its glycosylation consisting of sialylated and unsialylated Tn-antigens and core 1 and core 2 structures. The glycans on lubricin have also been suggested to be involved in crosslinking and stabilization of the lubricating superficial layer of cartilage by mediating interaction between lubricin and galectin-3. However, with the spectrum of glycans being found on lubricin, the glycan candidates involved in this interaction were unknown. Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3. Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding. Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens. These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.
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| 6. |
- Gourdon, Pontus Emanuel, 1978, et al.
(author)
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Optimized in vitro and in vivo expression of proteorhodopsin: A seven-transmembrane proton pump
- 2008
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In: Protein Expression and Purification. - : Elsevier BV. - 1096-0279 .- 1046-5928. ; 58:1, s. 103-113
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Journal article (peer-reviewed)abstract
- Proteorhodopsin is an integral membrane light-harvesting proton pump that is found in bacteria distributed throughout global surface waters. Here, we present a protocol for functional in vitro production of pR using a commercial cell-free synthesis system yielding 1.0 mg purified protein per milliliter of cell lysate. We also present an optimized protocol for in vivo over-expression of pR in Escherichia coli, and a two-step purification yielding 5 mg of essentially pure functional protein per liter of culture. Both approaches are straightforward, rapid, and easily scalable. Thus either may facilitate the exploitation of pR for commercial biotechnological applications. Finally, the implications of some observations of the in vitro synthesis behavior, as well as preliminary results towards a structural determination of pR are discussed.
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| 8. |
- Nyblom, Anna Maria, 1975, et al.
(author)
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Exceptional overproduction of a functional human membrane protein
- 2007
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In: Protein Expression and Purification. - : Elsevier BV. - 1046-5928 .- 1096-0279. ; 56:1, s. 110-120
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Journal article (peer-reviewed)abstract
- Eukaryotic-especially human-membrane protein overproduction remains a major challenge in biochemistry. Heterologously overproduced and purified proteins provide a starting point for further biochemical, biophysical and structural studies, and the lack of sufficient quantities of functional membrane proteins is frequently a bottleneck hindering this. Here, we report exceptionally high production levels of a correctly folded and crystallisable recombinant human integral membrane protein in its active form; human aquaporin 1 (hAQP1) has been heterologously produced in the membranes of the methylotrophic yeast Pichia pastoris. After solubilisation and a two step purification procedure, at least 90 mg hAQP1 per liter of culture is obtained. Water channel activity of this purified hAQP was verified by reconstitution into proteoliposomes and performing stopped-flow vesicle shrinkage measurements. Mass spectrometry confirmed the identity of hAQPI in crude membrane preparations, and also from purified protein reconstituted into proteoliposomes. Furthermore, crystallisation screens yielded diffraction quality crystals of untagged recombinant hAQP1. This study illustrates the power of the yeast P. pastoris as a host to produce exceptionally high yields of a functionally active, human integral membrane protein for subsequent functional and structural characterization. (c) 2007 Elsevier Inc. All rights reserved.
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| 9. |
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Analysing text AND talk : Att analysera texter OCH samtal
- 2014
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Editorial collection (other academic/artistic)abstract
- This report originates in two workshops on text and talk, one held in Sweden and one in Finland. It aims at exploring the intersections of text analysis and conversation analysis, and at highlighting the current themes of this field of research.List of contributions:Anna-Malin Karlsson & Henna Makkonen-Craig: Texts and conversations: traditions and perspectives from Sweden and FinlandLinnea Hanell & Mona Blåsjö: Diskurs i handling: Att studera människors handlingar med medierad diskursanalysSuvi Honkanen & Riikka Nissi: Managing organizational change through joint vision building: an analysis of practices of recontextualizationKarin Hagren Idevall & Theres Bellander: Membership Categorization Analysis – för analyser av kategoriseringar i tal och skriftAnna-Malin Karlsson: Evaluering för alla? Eller: Den farliga skriftenMats Landqvist: Positionering och validitet i enkäter och fokusgruppssamtalHenna Makkonen-Craig: Aspects of dialogicity: Exploring dynamic interrelations in written discourseHenna Makkonen-Craig: The emergence of a research tradition: Dialogically-oriented linguistic discourse analysis
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| 10. |
- Andersson, Fräs Annika, et al.
(author)
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Occurrence and abatement of volatile sulfur compounds during biogas production
- 2004
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In: Journal of the Air & Waste Management Association. - 1047-3289. ; 54:7, s. 855-861
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Journal article (peer-reviewed)abstract
- Volatile sulfur compounds (VSCs) in biogas originating from a biogas production plant and from a municipal sewage water treatment plant were identified. Samples were taken at various stages of the biogas-producing process, including upgrading the gas to vehicle-fuel quality. Solid-phase microextraction was used for preconcentration of the VSCs, which were subsequently analyzed using gas chromatography in combination with mass spectrometry. Other volatile organic compounds present also were identified. The most commonly occurring VSCs in the biogas were hydrogen sulfide, carbonyl sulfide, methanethiol, dimethyl sulfide, and dimethyl disulfide, and hydrogen sulfide was not always the most abundant sulfur (S) compound. Besides VSCs, oxygenated organic compounds were commonly present (e.g., ketones, alcohols, and esters). The effect of adding iron chloride to the biogas reactor on the occurrence of VSCs also was investigated. It was found that additions of 500-g/m3 substrate gave an optimal removal of VSCs. Also, the use of a prefermentation step could reduce the amount of VSCs formed in the biogas process. Moreover, in the carbon dioxide scrubber used for upgrading the gas, VSCs were removed efficiently, leaving traces (ppbv levels). The scrubber also removed other organic compounds.
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