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- Kehoe, Laura, et al.
(författare)
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Make EU trade with Brazil sustainable
- 2019
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 364:6438, s. 341-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Näslund, Jonas, 1979-, et al.
(författare)
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Detection of Puumala and Rift Valley Fever virus by quantitative RT-PCR and virus viability tests in samples of blood dried and stored on filter paper
- 2011
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Ingår i: Journal of Virological Methods. - Amsterdam : Elsevier. - 0166-0934 .- 1879-0984. ; 178:1-2, s. 186-90
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Tidskriftsartikel (refereegranskat)abstract
- Haemorrhagic fever viruses cause emerging infections worldwide, and blood or serum is the main sample used for diagnosis. However, storage and transportation of such samples from remote areas to regional laboratories may be complicated and expensive. In this study, a novel approach was evaluated for the detection of Puumala hantavirus (PUUV) RNA and Rift Valley fever virus (RVFV) RNA. Whole-blood samples spiked with viable virus particles were tested in parallel with clinical samples from patients with acute haemorrhagic fever with renal syndrome (nephropathia epidemica). Individual blood samples were spotted on filter paper, dried, and used for RNA extraction at later time points. PUUV RNA was detected by RT-PCR after storage at room temperature for up to six weeks. In contrast, only low copy numbers of RVFV RNA were detected after 1-2 days even though viable RVFV was eluted from the dried filter papers after the same time. The use of filter paper to collect and store blood samples for PUUV RNA detection is therefore a simple and reliable procedure. This approach might facilitate sampling and analysis of other RNA viruses from human or animal sources and could be used for field studies in remote areas or in developing countries.
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| 5. |
- Näslund, Jonas, 1979-, et al.
(författare)
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PCR detection of a hantavirus and Rift Valley fever virus using dried whole blood spotted on filter strips
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Viral hemorrhagic fevers are serious and emerging infections among humans and animals worldwide. Presently, blood or serum samples are the main source for diagnostics. However, transportation of such samples from remote areas may be complicated and expensive. Previously, filter strips soaked with blood have been used for detection of antibodies for diagnostics and epidemiological studies of several infectious diseases. In this study we evaluate if a similar approach could be applied for detection of viral RNA of Rift Valley Fever virus or Hantavirus (Puumala). We have used whole blood spiked with known amounts of viruses. In addition, clinical samples from patients with acute hemorrhagic fever with renal syndrome have been analysed. The samples were collected on filter strips and dried before RNA was extracted at different time-points. For Puumala, the sensitivity was acceptable, although the absolute levels of viral RNA were found to be considerable lower when using filter strips. The viral RNA could be detected and analysed after 2-3 weeks storage of the dried filter strips. In contrast, for RVFV, no or very low copy numbers of viral RNA were detected. Still, RVFV filter strips contained viable virus particles up to 48 h of storage. In conclusion, the use of dried whole blood samples spotted on filter strips for the detection of viral RNA seems to be a reliable and simple procedure for Hantaviruses. This procedure could be useful in field studies, especially in remote areas or low-income countries where transportation and storage of biological samples might be complicated. However, the result for RVFV was unexpected and further studies are needed to improve this technique.
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