| 1. |
- Abbas, Nasir, et al.
(author)
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Untargeted-metabolomics differentiation between poultry samples slaughtered with and without detaching spinal cord
- 2020
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In: Arabian Journal of Chemistry. - : ELSEVIER. - 1878-5352 .- 1878-5379. ; 13:12, s. 9081-9089
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Journal article (peer-reviewed)abstract
- Chicken meat is among the common and relatively inexpensive source of protein consumed worldwide from the poultry industry. Many communities show concern regarding the procedure of slaughtering animals for meat consumption due to ethical, religious, or cultural reasons. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) based untargeted metabolomics of 40 chicken meat samples were evaluated to differentiate meat samples based on slaughtering methods. Samples were grouped into, Zabiha (cutting neck without detaching spinal cord) and Non-Zabiha (completely detaching neck). A volcano plot reveals at least 150 features found significantly different between the two groups having >= 2-fold changes in intensities with p-values <= 0.05. Among them 05 identified and 25 unidentified metabolites have clear differences in peak intensities. The identified features can be employed to differentiate meat obtained from different slaughtering methods. A characteristic pattern based on principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA) was observed among the groups. The results will benefit Halal certification, food safety, and security agencies to curb food fraud. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud University.
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| 2. |
- Kumari, Sindhia, et al.
(author)
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Metabolomics approach to understand the hepatitis C virus induced hepatocellular carcinoma using LC-ESI-MS/MS
- 2021
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In: Arabian Journal of Chemistry. - : Elsevier. - 1878-5352 .- 1878-5379. ; 14:1
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Journal article (peer-reviewed)abstract
- Hepatocellular carcinoma (HCC) is a typical cancer that has region specified analysis with the incidence of hepatitis C virus (HCV) infection. This study was conducted to improve the understanding of metabolic alterations associated with HCV induced HCC which can open up new strategies to monitor the high risk of HCC. Samples of the subjects with HCV, HCV induced chronic liver disease (CLD), HCV induced HCC, and healthy controls (HS) were collected after complete blood count (CBC), hepatitis viral load, st-fetoprotein (AFP), liver function tests, and albumin. A total of 147 serum samples including HCC (n = 11), CLD (n = 24), HCV (n = 71), and HS (n = 41) were analyzed by LC-ESI-MS/MS. The 21 compounds were found to be responsible for group discrimination after the application of chemometric tools. Nfructosyl tyrosine and hydroxyindoleacetic acid showed an increase in level whereas L-aspartylL-phenylalanine and thyroxine showed a consistent decrease in the progression of HCV to HCC in comparison with HS indicating their importance for early detection. The biological pathways such as glycerophospholipid metabolism, phenylalanine, tyrosine, and tryptophan biosynthesis, phenylalanine metabolism and tryptophan metabolism showed alteration in some metabolites. The method was internally validated by ROC plot showing AUC value for HS, HCV, CLD, and HCC as 0.99, 1, 1, and 0.89, respectively; while 16 blind samples were also validated with 93% specificity. The untargeted metabolomics investigation of HCV, CLD, and HCC can help to understand the progression of HCV-induced HCC. It reveals significant differences in metabolites to predict prognostic and diagnostic markers. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
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| 3. |
- Usman, Muhammad, et al.
(author)
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Evaluation of the chronic intoxication of fluoride on human serum metabolome using untargeted metabolomics
- 2022
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In: Arabian Journal of Chemistry. - : Elsevier BV. - 1878-5352 .- 1878-5379. ; 15:7
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Journal article (peer-reviewed)abstract
- Drinking water is the main source of fluoride intake for the human body and its regulated consumption helps in decreasing dental caries. However, excessive fluoride consumption over a prolonged time period causes fluorosis disease which adversely affects many tissues and organs of the body. This paper describes the evaluation of chronic intoxication of fluoride on human serum metabolome. The untargeted metabolomics approach using UPLC-QTOF-MS/MS is applied for metabolomic profiling, whereas the estimation of fluoride in serum samples was carried out using the ion-selective electrode (ISE). Fluoride concentration was found to be 0.16–1.25 mg/L in serum samples of 39 fluorosis patients and 0.008–0.045 mg/L in 20 healthy samples. A total of 47 metabolites were identified based on the high-resolution mass spectrometry analysis. A volcano plot was generated to discriminate features that are significantly different between the fluorosis and healthy groups at the probability of 0.05 and fold change ≥ 2. Among all identified metabolites, intensities of ten differential identified metabolites including inosine, α-linolenic acid, guanosine, octanoyl-L-carnitine, His-Trp, phytosphingosine, lauroyl-L-carnitine, hydrocortisone, deoxyinosine and dodecanedioic acid have been found altered in disease samples compared to healthy controls. Major pathways identified based on these metabolites include energy metabolism, fatty acid oxidation, purine degradation pathway, elevated protein degradation, and increased ω-6 fatty acid linoleate signatures were observed.
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