| 1. |
- Andréasson, Maia, 1960, et al.
(author)
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På jakt efter amerikasvenskan
- 2013
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In: Svenskans beskrivning / [SvB.] 32, Förhandlingar vid trettioandra sammankomsten för svenskans beskrivning, Karlstad den 13-14 oktober 2011 / redigerade av Björn Bihl, Peter Andersson & Lena Lötmarker. - Karlstad : Karlstads universitet. - 1102-3619. - 9789170634901 ; 32, s. 82-91
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Conference paper (peer-reviewed)
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| 2. |
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| 3. |
- Boge, Lukas, 1987, et al.
(author)
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Cubosomes for topical delivery of the antimicrobial peptide LL-37
- 2019
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In: European Journal of Pharmaceutics and Biopharmaceutics. - : Elsevier BV. - 1873-3441 .- 0939-6411. ; 134, s. 60-67
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Journal article (peer-reviewed)abstract
- In this study, the use of cubosomes for topical delivery of the antimicrobial peptide (AMP) LL-37 was investigated. Topical delivery of AMPs is of great interest for treatment of skin infections caused by bacteria, such as Staphylococcus aureus. AMP containing cubosomes were produced by three different preparation protocols and compared: (i) pre-loading, where LL-37 was incorporated into a liquid crystalline gel, which thereafter was dispersed into nanoparticles, (ii) post-loading, where LL-37 was let to adsorb onto pre-formed cubosomes, and (iii) hydrotrope-loading, where LL-37 was incorporated during the spontaneously formed cubosomes in an ethanol/glycerol monooleate mixture. Particle size and size distribution were analyzed using dynamic light scattering (DLS), liquid crystalline structure by small angle x-ray scattering (SAXS) and release of LL-37 by a fluorescamine assay. Proteolytic protection of LL-37 as well as bactericidal effect after enzyme exposure was investigated. The skin irritation potential of cubosomes was examined by an in vitro epidermis model. Finally, the bacterial killing property of the cubosomes was examined by an ex vivo pig skin wound infection model with Staphylococcus aureus. Data showed that a high loading of LL-37 induced formation of vesicles in case of cubosomes prepared by sonication (pre-loading). No release of LL-37 was observed from the cubosomes, indicating strong association of the peptide to the particles. Proteolysis studies showed that LL-37 was fully protected against enzymatic attacks while associated with the cubosomes, also denoting strong association of the peptide to the particles. As a consequence, bactericidal effect after enzyme exposure remained, compared to pure LL-37 which was subjected to proteolysis. No skin irritation potential of the cubosomes was found, thus enabling for topical administration. The ex vivo wound infection model showed that LL-37 in pre-loaded cubosomes killed bacteria most efficient.
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| 4. |
- Hjalmarson, Jenny, 1974-
(author)
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Ergonomics at Home : Design for Safe Living and Home Care
- 2014
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Doctoral thesis (other academic/artistic)abstract
- The home should represent safety and security for the person who lives there, and this is an important factor for independence and autonomy in very old age. With aging populations, the needs for long-term care increase, care provided by spouses and/or from the growing home care sector. Injuries among these groups are common.In this study, an ergonomics perspective was applied in the analysis of some basic daily activities performed by old persons and by home care workers, assisting.The postures and movements of home care staff assisting at toilet visits, and transferring persons from wheelchair to toilet, were measured and analysed.Some daily activities related to making food and washing clothes, performed by a group of persons between the age of 75 and 100, were measured and analysed.The task of getting up from the floor – on your own and with the help of a walker equipped with a lifting device – was analysed with the help of older persons and nursing staff. The design was built on the knowledge gained from analysing how older people get up from floor.Requirements for access with a four-wheeled walker in the local built environment were investigated.Observations were made with the help of video recording. Postures were recorded with the CUELA measurement system. The VIDAR ergonomics evaluation instrument was used to register the participants’ experiences of discomfort and pain during getting up from the floor with or without the walker with a lifting device. Structured interviews were used to find out about older peoples’ experience of using the four-wheel walker.It is concluded that ageing at home requires improved architectural and technical bathroom design and improved access in the local built environment.
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| 5. |
- Holmberg, Anna, 1974, et al.
(author)
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Does waterborne citalopram affect the aggressive and sexual behaviour of rainbow trout and guppy?
- 2011
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In: Journal of hazardous materials. - : Elsevier BV. - 1873-3336 .- 0304-3894. ; 187:1-3, s. 596-9
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Journal article (peer-reviewed)abstract
- Citalopram is one of several selective serotonin reuptake inhibitors (SSRIs) commonly found in treated sewage effluents. Accordingly, there are concerns about possible adverse effects of SSRIs on aquatic organisms, particularly behavioural effects similar to those associated with SSRI use in humans. Rainbow trout fry and adult male guppies were therefore exposed to waterborne citalopram, ranging from environmentally relevant to high concentrations (1, 10, 100 μg/L) for 3-7 days. Under these experimental conditions citalopram does not appear to cause significant effects on aggression in rainbow trout fry or on sexual behaviour in male guppies. This may be explained by a relatively low uptake of citalopram from water to fish.
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| 6. |
- Jacobsson, Lars, et al.
(author)
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Det är hög tid att utveckla den psykiatriska heldygnsvården : Läkarna behöver involveras mer i avdelningarnas vardag
- 2021
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In: Läkartidningen. - 0023-7205 .- 1652-7518. ; 118
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Journal article (peer-reviewed)abstract
- Psychiatric care has changed dramatically from inpatient to outpatient care in the last decades. While a lot of resources have been spent on developing outpatient care, less interest has been invested in inpatient care, which has repeatedly been critisezed for being dominated by control, lack of collaboration between staff and patient and boredom. We suggest that there are three main functions of the psychiatric ward: the asylum function, the intensive care and the observational/diagnostic function. We also present some recent projects aimed at improving the quality of care and we argue for more involvment of the psychiatrists in the internal life and functioning of the psychiatric ward.
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| 7. |
- Larsson, Ida, 1977, et al.
(author)
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Amerikasvenskan förr och nu
- 2012
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In: Norsk lingvistisk tidskrift. - 0800-3076. ; 30:2, s. 263-286
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Journal article (peer-reviewed)abstract
- Denna artikel jämför dagens amerikasvenska med den som talades för 50 år sedan. Utifrån material som samlats in i Minnesota 2011 och material som samlades in under 1960-talet diskuteras dialektdrag, andraspråksdrag och, slutligen, vad som kan sägas utmärka den amerikasvenska som talas idag. Artikeln vill belysa ämnet ur flera möjliga infallsvinklar, blottlägga några av de processer som är inblandade och skissera några huvudlinjer i utvecklingen av amerikasvenskan.
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| 8. |
- Larsson, Jenny, 1974-, et al.
(author)
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Anti-inflammatory effects of a titanium-peroxy gel: role of oxygen metabolites and apoptosis.
- 2004
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In: Journal of biomedical materials research. Part A. - : Wiley. - 1549-3296 .- 0021-9304 .- 1097-4636. ; 68:3, s. 448-57
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Journal article (peer-reviewed)abstract
- Polymorphonuclear neutrophils (PMN) are among the first inflammatory cells to arrive at an implant interface, where they encounter with the foreign material and may produce reactive oxygen species (ROS). During the interaction between titanium and ROS, titanium-peroxy (Ti-peroxy) compounds may be formed. We used a Ti-peroxy gel, made from titanium and hydrogen peroxide, to study the effects of Ti-peroxy compounds on PMN. In the absence of serum, the Ti-peroxy gel decreased the oxidative response of PMN to yeast and PMA and reduced PMN apoptosis without inducing necrosis. These effects could not be ascribed to the release of hydrogen peroxide from the Ti-peroxy gel, because a steady-state hydrogen peroxide producing system failed to mimic the effects of the gel. The effects were similarly unaffected when PMN were preincubated with beta(2)-integrin antibodies, questioning the involvement of adhesion molecules. Nevertheless, when a filter was used to separate the Ti-peroxy gel from the cells, the gel effect on PMN life span was abolished, pointing to a contact-dependent mechanism. In the presence of serum, the Ti-peroxy gel had no effect on the PMN oxidative response and life span, but appeared rather inert. In summary, this study demonstrates that the Ti-peroxy gel has potentially anti-inflammatory properties through a combined peroxide and physical contact effect, supporting the notion that interactions between titanium and inflammatory cells are responsible for the good performance of titanium in vivo.
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| 9. |
- Larsson, Jenny, 1974-, et al.
(author)
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Involvement of the ß2-integrin CD18 in apoptosis signal transduction in human neutrophils
- 2000
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In: Inflammation Research. - : Springer Science and Business Media LLC. - 1023-3830 .- 1420-908X. ; 49:9, s. 452-459
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Journal article (peer-reviewed)abstract
- Objective and design: To examine the hypothesis that an accelerated rate of neutrophil apoptosis occurs following β2-integrin activation, and further investigate the signal transduction pathways involved.Material: Human polymorphonuclear neutrophils.Treatment: Neutrophils were challenged with pansorbins coated with antibodies towards the β2-integrin subunit CD18 in a proportion of 1:100 with or without the inhibitors diphenylene iodonium (10 M), cytochalasin B (5 μg/ml), genistein (10 nM), herbimycin A (10 M) and Z-VAD-FMK (10 μM).Methods: Measurement of phosphatidylserine exposure and DNA fragmentation in flow cytometry and assessment of H2O2-production through spectrofluorometry. The results were analysed using Mann Whitney U test and Kruskal Wallis.Results: Pansorbins coated with antibodies to CD18 induce apoptosis in neutrophils (p < 0.01), and activate the production of reactive oxygen species (p < 0.01). Pre-treatment with the inhibitors have no effect on anti-CD18 induced apoptosis.Conclusion: Anti-CD18 pansorbins induce apoptosis in neutrophils through an alternative pathway not involving reactive oxygen species and independent of tyrosine phosphorylation, cytoskeletal reorganisation and caspases.
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| 10. |
- Larsson, Jenny, 1974-
(author)
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Platelet-mediated inhibition of polymorphonuclear neutrophil apoptosis
- 2004
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Doctoral thesis (other academic/artistic)abstract
- Neutrophils play an important role in the cell-mediated immune response against invading micro-organisms. Upon infection, neutrophils in the bloodstream transmigrate into the tissue where they bind to and engulf the microbes. The infecting agent is then eliminated using reactive oxygen species (ROS) and proteolytic enzymes. It is crucial that the life span of activated neutrophils is tightly regulated since the prolonged release of ROS can cause severe damage to the surrounding tissue. Therefore, programmed cell suicide through apoptosis, and the subsequent ingestion of apoptotic neutrophils by neighbouring cells, are essential to the rapid resolution of inflammation.For the apoptotic process to work according to plan, an extensive control system is at work, instructing the neutrophil when and where to die. This system is influenced by intracellular as well as extracellular signals, that are conveyed through the neutrophil plasma membrane by cell adhesion molecules, for example integrins. The aim of this thesis was to investigate the involvement of adhesion molecules in the regulation of the neutrophil ROS production and apoptosis. We found that when neutrophils were challenged with particles exposing antibodies towards ß2-integrins they produced intracellular ROS, and this was dependent on reorganisation of the cytoskeleton. The particles also induced caspase-independent neutrophil apoptosis, and this ß2-integrin mediated signalling seemed to occur independently of tyrosine phosphorylation.Interaction of neutrophils with a potential biomaterial, a titanium-peroxy gel, reversibly inhibited ROS production and decreased spontaneous apoptosis. This effect was contact-dependent but involved cell signalling via adhesion molecules other than ß2-integrins. We also showed that platelet-induced inhibition of neutrophil apoptosis involved both ß2-integrins and carbohydrate structures, and that activated platelets were more efficient than resting platelets. Platelet membranes also inhibited neutrophil apoptosis, supporting the notion of adhesion molecule involvement.In conclusion, this thesis shows that adhesion molecules are involved in the regulation of neutrophil ROS production and apoptosis. Adhesion molecule signalling pathways may therefore be potential new therapeutic targets for treatment of diseases involving acute inflammation.
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