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| 2. |
- Bernfur, Katja, et al.
(author)
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Relative abundance of integral plasma membrane proteins in Arabidopsis leaf and root tissue determined by metabolic labeling and mass spectrometry.
- 2013
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In: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 8:8
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Journal article (peer-reviewed)abstract
- Metabolic labeling of proteins with a stable isotope ((15)N) in intact Arabidopsis plants was used for accurate determination by mass spectrometry of differences in protein abundance between plasma membranes isolated from leaves and roots. In total, 703 proteins were identified, of which 188 were predicted to be integral membrane proteins. Major classes were transporters, receptors, proteins involved in membrane trafficking and cell wall-related proteins. Forty-one of the integral proteins, including nine of the 13 isoforms of the PIP (plasma membrane intrinsic protein) aquaporin subfamily, could be identified by peptides unique to these proteins, which made it possible to determine their relative abundance in leaf and root tissue. In addition, peptides shared between isoforms gave information on the proportions of these isoforms. A comparison between our data for protein levels and corresponding data for mRNA levels in the widely used database Genevestigator showed an agreement for only about two thirds of the proteins. By contrast, localization data available in the literature for 21 of the 41 proteins show a much better agreement with our data, in particular data based on immunostaining of proteins and GUS-staining of promoter activity. Thus, although mRNA levels may provide a useful approximation for protein levels, detection and quantification of isoform-specific peptides by proteomics should generate the most reliable data for the proteome.
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| 3. |
- Kuja-Halkola, Ralf, et al.
(author)
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Do borderline personality disorder and attention-deficit/hyperactivity disorder co-aggregate in families? : A population-based study of 2 million Swedes
- 2021
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In: Molecular Psychiatry. - : Nature Publishing Group. - 1359-4184 .- 1476-5578. ; 26:1, s. 341-349
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Journal article (peer-reviewed)abstract
- Large-scale family studies on the co-occurrence of attention-deficit/hyperactivity disorder (ADHD) and borderline personality disorder (BPD) are lacking. Thus, we aimed to estimate the co-occurrence and familial co-aggregation of clinically ascertained ADHD and BPD diagnoses using the entire Swedish population. In a register-based cohort design we included individuals born in Sweden 1979-2001, and identified their diagnoses during 1997-2013; in total, 2,113,902 individuals were included in the analyses. We obtained clinical diagnoses of ADHD and BPD from inpatient and outpatient care. Individuals with an ADHD diagnosis had an adjusted (for birth year, sex, and birth order) odds ratio (aOR) of 19.4 (95% confidence interval [95% CI] = 18.6-20.4) of also having a BPD diagnosis, compared to individuals not diagnosed with ADHD. Having a sibling with ADHD also increased the risk for BPD (monozygotic twins, aOR = 11.2, 95% CI = 3.0-42.2; full siblings, aOR = 2.8, 95% CI = 2.6-3.1; maternal half-siblings, aOR = 1.4, 95% CI = 1.2-1.7; paternal half-siblings, aOR = 1.5, 95% CI = 1.3-1.7). Cousins also had an increased risk. The strength of the association between ADHD and BPD was similar in females and males, and full siblings showed similar increased risks regardless of sex. Among both males and females, ADHD and BPD co-occur within individuals and co-aggregate in relatives; the pattern suggests shared genetic factors and no robust evidence for etiologic sex differences was found. Clinicians should be aware of increased risks for BPD in individuals with ADHD and their relatives, and vice versa.
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- Larsson, Niklas, et al.
(author)
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Continuous flow hollow fiber liquid-phase microextraction and monitoring of NSAID pharmaceuticals in a sewage treatment plant effluent
- 2009
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In: Analytical Methods. - : Royal Society of Chemistry (RSC). - 1759-9660 .- 1759-9679. ; 1:1, s. 59-67
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Journal article (peer-reviewed)abstract
- A method for simultaneous extraction and quantification Of four non-steroidal anti-inflammatory drugs (NSAIDs) based on continuous flow hollow fiber liquid-phase microextraction (CFHF-LPME) was developed. The effect of sample flow rate, acceptor flow rate, type of acceptor flow (continuous, semi-continuous or forward-backward), type of supported liquid membrane and sample volume was studied. The extraction of the final method was linear over an environmentally relevant concentration range and yielded high enrichment factors (720-940 times) in reagent water and (270-800 times) in sewage water for all analytes within 45 min. Repeatability was best (RSD 6-15%) during the first 30 min of extraction. The optimised method was used to monitor the occurrence and fate of the four NSAIDs in a Swedish sewage treatment plant (STP) effluent, which is discharged into a system of ponds before release into a river, during the period May-September 2008. All four analytes were detected at concentrations up to 0.92 mu g L-1 ketoprofen, 0.08 mu g L-1 naproxen, 0.43 mu g L-1 diclofenac and 0.25 mu g L-1 ibuprofen. A concentration drop during the summer was observed. For diclofenac and ketoprofen significant removal in the primary recipient pond system was observed. The presence of the studied pharmaceuticals in STP effluent together with concern about their environmental effects makes monitoring of their occurrence and knowledge of their environmental fate important. The proposed method provides a basis for automation of extraction towards on-site extraction using CFHF-LPME.
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| 6. |
- Pisani, Luigi, et al.
(author)
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Geoeconomic variations in epidemiology, ventilation management, and outcomes in invasively ventilated intensive care unit patients without acute respiratory distress syndrome : a pooled analysis of four observational studies
- 2022
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In: The Lancet Global Health. - 2214-109X. ; 10:2, s. 227-235
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Journal article (peer-reviewed)abstract
- Background: Geoeconomic variations in epidemiology, the practice of ventilation, and outcome in invasively ventilated intensive care unit (ICU) patients without acute respiratory distress syndrome (ARDS) remain unexplored. In this analysis we aim to address these gaps using individual patient data of four large observational studies. Methods: In this pooled analysis we harmonised individual patient data from the ERICC, LUNG SAFE, PRoVENT, and PRoVENT-iMiC prospective observational studies, which were conducted from June, 2011, to December, 2018, in 534 ICUs in 54 countries. We used the 2016 World Bank classification to define two geoeconomic regions: middle-income countries (MICs) and high-income countries (HICs). ARDS was defined according to the Berlin criteria. Descriptive statistics were used to compare patients in MICs versus HICs. The primary outcome was the use of low tidal volume ventilation (LTVV) for the first 3 days of mechanical ventilation. Secondary outcomes were key ventilation parameters (tidal volume size, positive end-expiratory pressure, fraction of inspired oxygen, peak pressure, plateau pressure, driving pressure, and respiratory rate), patient characteristics, the risk for and actual development of acute respiratory distress syndrome after the first day of ventilation, duration of ventilation, ICU length of stay, and ICU mortality. Findings: Of the 7608 patients included in the original studies, this analysis included 3852 patients without ARDS, of whom 2345 were from MICs and 1507 were from HICs. Patients in MICs were younger, shorter and with a slightly lower body-mass index, more often had diabetes and active cancer, but less often chronic obstructive pulmonary disease and heart failure than patients from HICs. Sequential organ failure assessment scores were similar in MICs and HICs. Use of LTVV in MICs and HICs was comparable (42·4% vs 44·2%; absolute difference –1·69 [–9·58 to 6·11] p=0·67; data available in 3174 [82%] of 3852 patients). The median applied positive end expiratory pressure was lower in MICs than in HICs (5 [IQR 5–8] vs 6 [5–8] cm H2O; p=0·0011). ICU mortality was higher in MICs than in HICs (30·5% vs 19·9%; p=0·0004; adjusted effect 16·41% [95% CI 9·52–23·52]; p<0·0001) and was inversely associated with gross domestic product (adjusted odds ratio for a US$10 000 increase per capita 0·80 [95% CI 0·75–0·86]; p<0·0001). Interpretation: Despite similar disease severity and ventilation management, ICU mortality in patients without ARDS is higher in MICs than in HICs, with a strong association with country-level economic status. Funding: No funding.
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| 8. |
- Werner, Karl, et al.
(author)
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Moving the PAR reduction criterion into the line driver
- 2015
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In: European Signal Processing Conference. - : European Signal Processing Conference, EUSIPCO. ; , s. 1939-1942, s. 1939-1942
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Conference paper (peer-reviewed)abstract
- Traditionally, Peak to Average Ratio (PAR) reduction in digital subscriber line (DSL) transmitters focuses on a digital-domain signal, either at the output of the baseband processing block or at the input of the digital-to-analogue converter (DAC). However, analysis of a DSL transceiver shows that the power dissipation is highly dependent on the PAR at a certain node inside the line driver. Thus, in order to be fully effective, the algorithm design should include the power amplifier dynamics. A typical, actively terminated, line driver is analysed and a model is constructed for PAR reduction purposes. The PAR reduction algorithm is then extended to take advantage of the model. Simulations show that algorithms which are designed to reduce PAR at the new, physically motivated, node obtain about 0.5 dB lower PAR evaluated at this node compared to methods that focus on the PAR of the DAC input.
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| 9. |
- Ahlgren, Niklas, et al.
(author)
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PRISMA Mission Extension : Adapting Mission Operations to New and Changing Mission Objectives
- 2012
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In: SpaceOps 2012 Conference. - Reston, Virigina : American Institute of Aeronautics and Astronautics.
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Conference paper (peer-reviewed)abstract
- The PRISMA in-orbit test-bed was launched in June 2010 to demonstrate strategies and technologies for formation flying and rendezvous. OHB Sweden is the prime contractor for the project which is funded by the Swedish National Space Board (SNSB) with support from DLR, CNES, and DTU. In early September of 2011, 15 months after launch, all primary mission objectives of the PRISMA formation flying satellites had been achieved and mission success was declared. Since a significant amount of delta-V capability still remained an open call for new experiments was issued, inviting both old and new experimenters to use the capabilities of the formation. Several interested parties took the opportunity to perform their own experiments with an existing platform, each coming with new mission objectives not previously planned to be flown on the PRISMA satellites. Some of these experiments were close to what had already been achieved within the nominal mission, but some included new ways of using the formation not envisioned by the spacecraft designers. The new experiments span from data collection in specific relative orbits, with a separation from a few meters to several kilometers, to entirely new modules within the on-board software. Changing from a pre-planned technology demonstration mission to operating a commercial resource required adaptation of the original operational concept, taking into account the different levels of experience of the customers and managing the satellites between experiments. This paper describes how these new mission objectives were integrated in operations and how a sometimes very short turn-around between initial concept and experiment execution was implemented with the aid of well established validation processes, high degrees of on-board autonomy and a flexible operations team.
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| 10. |
- Ahrén, Irini Lazou, et al.
(author)
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Fewer Community-Acquired Colds with Daily Consumption of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2. A Randomized, Placebo-Controlled Clinical Trial
- 2021
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In: The Journal of nutrition. - : Elsevier BV. - 1541-6100 .- 0022-3166. ; 151:1, s. 214-222
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Journal article (peer-reviewed)abstract
- BACKGROUND: Viral infections of the upper airways are the most common cause for absence from work or school, and there is evidence for probiotic efficacy in reducing the incidence and severity of these infections.OBJECTIVES: We aimed to confirm the previously reported beneficial effects of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 against community-acquired common colds and identify a possible mechanism of action.METHODS: In a double-blind study, healthy adults (18-70 years of age) with at least 4 colds during the last 12 months before recruitment were randomly allocated to consume either probiotics (n = 448; total daily dose of 109 CFU with the 2 strains equally represented) or placebo (n = 450) once daily for 12 weeks. Recruitment took place from October to February during 2013-2016 (over 3 cold seasons). The probiotic impact on the severity of the colds (Wisconsin Upper Respiratory Symptom Survey-21) was the primary endpoint, whereas secondary endpoints included the incidence rate and duration of colds and an analysis of immune markers. Mann-Whitney U test and mixed model were used for the analysis of continuous variables and Fisher´s exact test was used for the analysis of categorical endpoints.RESULTS: Symptom severity was not reduced after intake of the probiotic, despite the positive trend seen in the first season. However, significantly fewer colds were experienced in the probiotic group (mean of 1.24 colds) as compared to the placebo group (mean of 1.36 colds; P = 0.044) for subjects reporting at least 1 cold, the incidence of recurring colds was 30% lower (20.8% vs. 29.8%, respectively; P = 0.055), and the use of analgesics was 18% lower (26.3% vs. 32%, respectively; P = 0.07). After 12 weeks, the change from baseline for IFN-γ differed between the groups (mean difference of -7.01; 95% CI, -14.9 to 0.93; P = 0.045).CONCLUSIONS: Intake of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 can be protective against multiple colds in adults prone to getting colds.This trial was registered at clinicaltrials.gov as NCT02013934.
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