| 1. |
- Ezzat, Kariem, et al.
(author)
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Scavenger receptor-mediated uptake of cell-penetrating peptide nanoparticles with oligonucleotides
- 2012
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In: The FASEB Journal. - : Wiley. - 0892-6638 .- 1530-6860. ; 26:3, s. 1172-1180
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Journal article (peer-reviewed)abstract
- Cell-penetrating peptides (CPPs) are shortcationic peptides that penetrate cells by interacting withthe negatively charged plasma membrane; however, thedetailed uptake mechanism is not clear. In contrary to theconventional mode of action of CPPs, we show here thata CPP, PepFect14 (PF14), forms negatively charged nanocomplexeswith oligonucleotides and their uptake is mediatedby class-A scavenger receptors (SCARAs). Specificinhibitory ligands of SCARAs, such as fucoidin, polyinosinicacid, and dextran sulfate, totally inhibit the activityof PF14-oligonucleotide nanocomplexes in the HeLapLuc705 splice-correction cell model, while nonspecific,chemically related molecules do not. Furthermore, RNAinterference (RNAi) knockdown of SCARA subtypes(SCARA3 and SCARA5) that are expressed in this cell lineled to a significant reduction of the activity to <50%. Inline with this, immunostaining shows prevalent colocalizationof the nanocomplexes with the receptors, and electronmicroscopy images show no binding or internalizationof the nanocomplexes in the presence of theinhibitory ligands. Interestingly, naked oligonucleotidesalso colocalize with SCARAs when used at high concentrations.These results demonstrate the involvement ofSCARA3 and SCARA5 in the uptake of PF14-oligonucleotidenanocomplexes and suggest for the first time thatsome CPP-based systems function through scavenger receptors,which could yield novel possibilities to understandand improve the transfection by CPPs.
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| 2. |
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"...men ingen berättar om de fula sidorna..."Rapport från en FoU-cirkel för lokal mobilisering mot cannabis
- 2013
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Editorial collection (other academic/artistic)abstract
- Det finns många myter, fördomar och föreställningar kring cannabis, som är det mest använda narkotiska preparatet i Europa1. En av dem låter ungefär så här: – Cannabis, det växer ju i naturen, det kan väl inte vara farligt eller skadligt, det är ju naturligt, så då måste det väl vara ok. Men spice, det är ju konstgjord skit, det kan man inte ta, det är ju livsfarligt. Myten är, åtminstone när det gäller att avstå spice, dåligt förankrad i verkligheten, så som myter brukar vara. Tullens beslag av syntetiska droger ökar hela tiden och i topp ligger kemiskt framställd cannabis. I dagsläget finns 31 olika sorter spice, som alla är narkotikaklassade. Tillsammans med cannabis och hasch utgör de lejonparten av alla tullens beslag när det gäller droger som beställs via nätet och som kommer till sina beställare via posten. Till det kommer den mer traditionella smugglingen av drogerna med tillhörande gatulangning. Hemodlandet av cannabis både i form av enstaka plantor på fönsterbrädan eller i garderoben för eget bruk, och i form av ”yrkesmässig” odling för försäljning, verkar också öka. Enligt Tullverket ökar också shoppingturismen över Öresund, särskilt inför och under helger, när många åker över till Danmark för att fylla på sina egna lager av cannabis och cannabisharts2. Tillgången på såväl odlad cannabis som syntetiskt framställd sådan är med andra ord god, och cannabis är den vanligaste illegala drogen bland ungdomar i Sverige, och användningen ökar, hela tiden. Kunskapsläget kring cannabisanvändandet varierar från de riktigt insatta till dem som inte är riktigt lika insatta och som därför lätt förirrar sig in i myternas töcken. En del av den kunskap som finns har vi samlat i den här FoU-rapporten till stöd för en regional mobilisering mot cannabis i Västra Götaland.
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| 3. |
- Reicher, Stephen D., et al.
(author)
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The EASP Diversity Report : Round Table Discussion
- 2017
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Conference paper (other academic/artistic)abstract
- EASP is committed to promote and support all types of diversity within the association—gender, geographic, thematic, and methodological—and to facilitate a supportive and inclusive environment for members from a diverse membership. To ensure this goal is achieved, the Executive Committee has commissioned an analysis of the past and present situation regarding diversity within the organization across all our activities (awards, grants, meetings, etc.). A Diversity Working Party (WP) has collected and analyzed existing data to determine whether there is a need for more systematic analyses into barriers to and facilitators of diversity in EASP. In this GM session, the WP presents and discusses key findings from this project. The results include analyses of membership over time broken down by geography, gender, age, and level; data on meeting participation, Presidents, EC members, prize winners, meeting organizers, and journal editors/associate editors/editorial boards broken down by the same categories plus (where appropriate) thematic and methodological approach. The audience is invited to participate in a discussion of conclusions and practical proposals for increasing the diversity within the organization.
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| 4. |
- Borendal Wodlin, Ninnie, 1962-, et al.
(author)
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Influence of Regional Analgesia on Self-Reported Quality of Sleep After Gynecological Abdominal Surgery : A Secondary Analysis of a Randomized Trial
- 2024
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In: Journal of Obstetrics and Gynaecology Canada. - : Elsevier Inc.. - 1701-2163. ; 46:1
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Journal article (peer-reviewed)abstract
- Objectives: To determine whether intrathecal morphine (ITM) analgesia in abdominal surgery for presumed gynecological malignancy was associated with better self-reported sleep quality postoperatively compared with epidural analgesia (EDA), and to evaluate risk factors for bad sleep quality. Methods: A secondary analysis of a randomized open controlled trial, comparing ITM and EDA as postoperative analgesia in 80 women undergoing laparotomy under general anaesthesia in an enhanced recovery after surgery framework. A total of 38 women allocated to ITM and 39 to EDA completed the study. The Swedish Postoperative Symptoms Questionnaire assessed symptoms and sleep quality during the first postoperative week. Multiple logistic regression models evaluated risk factors. The results are presented as adjusted odds ratios with 95% CIs. Results: The sleep quality night-by-night did not differ significantly between the women who had ITM or EDA. Risk factors for bad sleep quality for night 1 were age (0.91; 0.84–0.99), operation time (1.02; 1.00–1.03), and opioid consumption (0.96; 0.91–0.99). For night 2, regular use of hypnotics preoperatively (15.81; 1.52–164.27) and opioid consumption (1.07; 1.00–1.14) were independent risk factors for bad sleep. After the second night, no risk factors were disclosed. Conclusions: ITM and EDA did not appear to affect the sleep quality postoperatively differently in women undergoing laparotomy for presumed gynecological malignancy. Risk factors for self-reported bad sleep quality varied during the first 3 days after surgery. Younger age, longer operation time, and preoperative use of hypnotics were associated with bad sleep quality, whereas the effect of opioid consumption on sleep quality varied depending on the time since surgery. These findings merit further studies. © 2023 The Society of Obstetricians and Gynaecologists of Canada/La Société des obstétriciens et gynécologues du Canada
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| 5. |
- Colins, Olivier F., et al.
(author)
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The Prognostic Usefulness of Multiple Specifiers for Subtyping Conduct Problems in Early Childhood
- 2023
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In: Journal of the American Academy of Child and Adolescent Psychiatry. - : Elsevier. - 0890-8567 .- 1527-5418. ; 62:10S, s. S160-S161
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Journal article (other academic/artistic)abstract
- Objectives: Better descriptions and treatment of children with conduct problems (CP) grandiose-manipulative (GM) and daring-impulsive (DI) traits are proposed for subtyping CP, instead of only using a callous-unemotional (CU) specifier. Unfortunately, the acclaimed benefits of having multiple specifiers for CP remain largely untested and, therefore, highly speculative. To fill this gap, we tested longitudinal relations between these 3 specifiers and developmental outcomes in childhood and adolescence, independent of early childhood CP.Methods: We relied on longitudinal data from 2 community studies. Teacher-ratings were used to measure CP and the specifiers in 3- to 5-year-olds from Sweden (N = 2064) and Spain (N = 2055). Developmental outcomes were assessed by multiple informants (ie, teachers, parents, and children) 1 to 8 years later.Results: Early childhood CP were predictive of all outcomes. CU traits predicted low levels of social competence and prosocial behavior, independent of CP (and age, gender, and socioeconomic status). GM and DI traits were predictive of aggression and violent delinquency, respectively, independent of CP, but also of higher levels of prosocial behavior or social competence.Conclusions: The 3 specifiers are predictive of different outcomes, independent of CP, which is thought to form the basis for developing specifiers for CP. Findings tentatively challenge the centrality of CU traits for subtyping CP, but it is premature to conclude that GM and DI specifiers are needed in future revisions of the DSM and ICD. Efforts to systematically evaluate the utility of these specifiers should be welcomed to inform ongoing debates on this matter.
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| 6. |
- Diab, Joseph, et al.
(author)
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Mucosal Metabolomic Profiling and Pathway Analysis Reveal the Metabolic Signature of Ulcerative Colitis
- 2019
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In: Metabolites. - : MDPI. - 2218-1989 .- 2218-1989. ; 9:12
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Journal article (peer-reviewed)abstract
- The onset of ulcerative colitis (UC) is characterized by a dysregulated mucosal immune response triggered by several genetic and environmental factors in the context of host–microbe interaction. This complexity makes UC ideal for metabolomic studies to unravel the disease pathobiology and to improve the patient stratification strategies. This study aims to explore the mucosal metabolomic profile in UC patients, and to define the UC metabolic signature. Treatment- naïve UC patients (n = 18), UC patients in deep remission (n = 10), and healthy volunteers (n = 14) were recruited. Mucosa biopsies were collected during colonoscopies. Metabolomic analysis was performed by combined gas chromatography coupled to time-of-flight mass spectrometry (GC-TOF-MS) and ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS). In total, 177 metabolites from 50 metabolic pathways were identified. The most prominent metabolome changes among the study groups were in lysophosphatidylcholine, acyl carnitine, and amino acid profiles. Several pathways were found perturbed according to the integrated pathway analysis. These pathways ranged from amino acid metabolism (such as tryptophan metabolism) to fatty acid metabolism, namely linoleic and butyrate. These metabolic changes during UC reflect the homeostatic disturbance in the gut, and highlight the importance of system biology approaches to identify key drivers of pathogenesis which prerequisite personalized medicine.
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| 7. |
- Pappaterra, Mauro José
(author)
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A Literature Review for the Application of Artificial Intelligence in the Maintenance of Railway Operations with an Emphasis on Data
- 2022
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In: DEPENDABLE COMPUTING, EDCC 2022 WORKSHOPS. - Cham : Springer Nature. - 9783031162459 - 9783031162442 ; , s. 59-75
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Conference paper (peer-reviewed)abstract
- This literature review aims for a holistic overview of Artificial Intelligence (AI) applications in the railway industry. Our research covers specifically the subdomain of railway maintenance. We have analyzed the state of the art of AI applied to the railway industry by conducting an extensive literature review, summarizing different tasks and problems belonging to railway maintenance and common AI-based models implemented for their solution. Within this study we present an integrated overview with special emphasis on the data used to create these models. The results of our research show that the possible applications of AI for the maintenance of railway operations are vast and there are many problems and tasks that can greatly benefit from it. Moreover, very different types of data are implemented to feed AI models: including not only numerical, text and image data but a wide variety of data types ranging from sound, GPS coordinates, track geometry, speed and acceleration data, data from rolling stock vibrations, knowledge from experts, historical data from logs and reports, temperature data and more. Data can also be harvested using different technologies such as IoT devices, wireless networks, smart sensors, computer-based simulations, and digital twins. These and more insights are discussed in detail within this paper.
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| 8. |
- Blanck, Rasmus, 1982
(author)
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Hierarchical incompleteness results for arithmetically definable extensions of fragments of arithmetic
- 2021
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In: The Review of Symbolic Logic. - 1755-0203 .- 1755-0211. ; 14:3, s. 624-644
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Journal article (peer-reviewed)abstract
- There has been a recent interest in hierarchical generalisations of classic incompleteness results. This paper provides evidence that such generalisations are readily obtainable from suitably formulated hierarchical versions of the principles used in the original proofs. By collecting such principles, we prove hierarchical versions of Mostowski’s theorem on independent formulae, Kripke’s theorem on flexible formulae, Woodin’s theorem on the universal algorithm, and a few related results. As a corollary, we obtain the expected result that the formula expressing “T is Σn-ill” is a canonical example of a Σn+1formula that is Πn+1-conservative over T.
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| 9. |
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| 10. |
- Lu, Yonggang, et al.
(author)
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1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction.
- 2023
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In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 120:8
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Journal article (peer-reviewed)abstract
- Sperm acrosomal membrane proteins, such as Izumo sperm-egg fusion 1 (IZUMO1) and sperm acrosome-associated 6 (SPACA6), play essential roles in mammalian gamete binding or fusion. How their biosynthesis is regulated during spermiogenesis has largely remained elusive. Here, we show that 1700029I15Rik knockout male mice are severely subfertile and their spermatozoa do not fuse with eggs. 1700029I15Rik is a type-II transmembrane protein expressed in early round spermatids but not in mature spermatozoa. It interacts with proteins involved in N-linked glycosylation, disulfide isomerization, and endoplasmic reticulum (ER)-Golgi trafficking, suggesting a potential role in nascent protein processing. The ablation of 1700029I15Rik destabilizes non-catalytic subunits of the oligosaccharyltransferase (OST) complex that are pivotal for N-glycosylation. The knockout testes exhibit normal expression of sperm plasma membrane proteins, but decreased abundance of multiple acrosomal membrane proteins involved in fertilization. The knockout sperm show upregulated chaperones related to ER-associated degradation (ERAD) and elevated protein ubiquitination; strikingly, SPACA6 becomes undetectable. Our results support for a specific, 1700029I15Rik-mediated pathway underpinning the biosynthesis of acrosomal membrane proteins during spermiogenesis.
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