| 1. |
- Alexanderson, Camilla, 1978, et al.
(author)
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A single early postnatal estradiol injection affects morphology and gene expression of the ovary and parametrial adipose tissue in adult female rats.
- 2010
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In: The Journal of steroid biochemistry and molecular biology. - : Elsevier BV. - 1879-1220 .- 0960-0760. ; 122:1-3, s. 82-90
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Journal article (peer-reviewed)abstract
- Events during early life can affect reproductive and metabolic functions in adulthood. We evaluated the programming effects of a single early postnatal estradiol injection (within 3h after birth) in female rats. We assessed ovarian and parametrial adipose tissue morphology, evaluated gene expression related to follicular development and adipose tissue metabolism, and developed a non-invasive volumetric estimation of parametrial adipose tissue by magnetic resonance imaging. Estradiol reduced ovarian weight, increased antral follicle size and number of atretic antral follicles, and decreased theca interna thickness in atretic antral follicles. Adult estradiol-injected rats also had malformed vaginal openings and lacked corpora lutea, confirming anovulation. Estradiol markedly reduced parametrial adipose tissue mass. Adipocyte size was unchanged, suggesting reduced adipocyte number. Parametrial adipose tissue lipoprotein lipase activity was increased. In ovaries, estradiol increased mRNA expression of adiponectin, complement component 3, estrogen receptor alpha, and glucose transporter 3 and 4; in parametrial adipose tissue, expression of complement component 3 was increased, expression of estrogen receptor alpha was decreased, and expression of leptin, lipoprotein lipase, and hormone-sensitive lipase was unaffected. These findings suggest that early postnatal estradiol exposure of female rats result in long-lasting effects on the ovary and parametrial adipose tissue at adult age.
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| 2. |
- Bjursten, Sara, et al.
(author)
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Concentrations of S100B and neurofilament light chain in blood as biomarkers for checkpoint inhibitor-induced CNS inflammation
- 2024
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In: EBioMedicine. - 2352-3964. ; 100
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Journal article (peer-reviewed)abstract
- Background Cancer treatment with immune checkpoint inhibition (ICI) can cause immune -related adverse events in the central nervous system (CNS irAE). There are no blood biomarkers to detect CNS irAE. We investigated if concentrations of S100 -calcium -binding protein B (S100B) and neurofilament light chain (NfL) in blood can be used as biomarkers for CNS irAE and assessed the incidence of CNS irAE in a cohort of ICI -treated patients. Methods In this single -centre, retrospective cohort study, we examined medical records and laboratory data of 197 consecutive patients treated with combined CTLA-4 and PD -1 inhibition (ipilimumab; ipi + nivolumab; nivo) for metastatic melanoma or renal cell carcinoma. CNS irAE was diagnosed using established criteria. Concentrations of S100B and NfL in blood were measured in patients with CNS irAE and in 84 patients without CNS irAE. Findings Nine of 197 patients (4.6%) fulfilled criteria for CNS irAE. S100B and NfL in blood increased during CNS inflammation and normalized during immunosuppression. CNS irAE was detected with a sensitivity of 100% (S100B) and 79% (NfL) and a specificity of 89% (S100B) and 74% (NfL). Patients with CNS irAE had simultaneous increased concentration of C -reactive protein (CRP) (9/9) and alanine aminotransferase (ALT) and/or aspartate aminotransferase (AST) in blood (8/9). Interpretation Analysis of S100B, NfL and CRP in blood facilitates the diagnosis of CNS irAE. CNS irAE may be more common than previously reported. There may be shared immune mechanisms between CNS and hepatitis irAE.
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| 3. |
- El-Sayed, Najib M., et al.
(author)
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The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
- 2005
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In: Science. - : American Association for the Advancement of Science (AAAS). - 1095-9203 .- 0036-8075. ; 309:5733, s. 409-15
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Journal article (peer-reviewed)abstract
- Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs. Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. Analyses of the T. cruzi, T. brucei, and Leishmania major (Tritryp) genomes imply differences from other eukaryotes in DNA repair and initiation of replication and reflect their unusual mitochondrial DNA. Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention.
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| 4. |
- Levin, Joel, et al.
(author)
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Multiple independent origins of intermediate species between Sorbus aucuparia and S. hybrida (Rosaceae) in the Baltic region
- 2018
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In: Nordic Journal of Botany. - : Wiley. - 0107-055X. ; 36:12
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Journal article (peer-reviewed)abstract
- Populations intermediate between the diploid and sexual Sorbus aucuparia and the tetraploid and facultative apomictic Sorbus hybrida are scattered in coastal regions of southern Scandinavia. Our aims were to investigate whether these populations were of local and independent origins, whether they were morphologically and molecularly distinct from each other and whether they could give rise to constant offspring by apomixis. Six intermediate populations from the Baltic Sea basin were studied for variation at nuclear and plastid microsatellite loci, for morphological differentiation patterns, and for ploidy level by flow cytometry. Seeds were sown to check for constancy with the mother plants. Three populations from Gotland and possibly the small population from Uppland were in each case fixed for a unique combination of genetic markers, had restricted variation in leaf shape and were morphologically divergent from each other. Flow cytometry analysis showed that the four genetically monomorphic populations were consistently triploid. Cultivation showed that offspring from trees within two of the monomorphic populations had leaf shapes closely similar to the mother individuals. In contrast, a population from Gotland and a population from Åland were variable at molecular marker loci and were variable in leaf shape. The three monomorphic Gotland populations and possibly also the Uppland population propagate asexually by apomixis, and they have independent origins. The two variable populations are apparently composed of hybrids that have been formed repeatedly and independently between individuals of the two parental species growing at the same sites. One of the Gotland apomicts has already been described as Sorbus teodori. Sorbus faohraei Hedrén & J.Levin and Sorbus atrata Hedrén & J.Levin are described as new apomictic species from Gotland.
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| 5. |
- Persson, Tomas, et al.
(author)
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Kungsmarken — en lägesrapport
- 2023
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In: Botaniska Notiser. - 0006-8195. ; 156:2, s. 1-10
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Journal article (other academic/artistic)
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| 6. |
- Thell, Arne, et al.
(author)
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Eslövs ansvarsarter i LONA-projekt
- 2023
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In: Botaniska Notiser: utgivna av Lunds botaniska förening (2001-). - 1650-3767. ; 156:1, s. 9-33
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Journal article (other academic/artistic)abstract
- A LONA (local nature conservation support) project administrated by Eslöv town in cooperation with Lund Botanical Society was undertaken in 2020– 2022 to determine the status of the protected plant species Betony (Betonica officinalis L.) and Marsh Crane’s-bill (Geranium palustre L.) in natural popu- lations. The second main goal of the project was to establish the species in planted back-up localities. Saw-wort (Serratula tinctoria L.) and Fen Ragwort (Jacobaea paludosa (L.) P. Gaertn.) were also inclu- ded in the project. The approximate number of spon- taneous specimens within the municipal boundary in 2021 were 76, 290, 47 and 300 respectively for Be- tony, Marsh Crane’s-bill, Saw-wort and Fen ragwort. In 2022, of 342 Betonies planted in 2017–2022, 264 were present, 27 of the 51 planted Marsh Crane’s- bills and 11 of the 59 planted Saw-worts were pre- sent, while 86 Fen ragworts had increased naturally to 124. Stehag material of Betony is present at all five back-up localities established within the Eslöv town area dedicated to the LONA project. There are also two previously established back-up localities for Betony from Kastberga Meadow along Harjagersvä- gen and Abullahagen nature reserve in Eslöv, where at least 63 and four specimens respectively were ob- served. In addition, Betony’s three other provenan- ces in Eslöv are represented in Botanical Gardens outside the municipality border, namely Botanical Garden of Lund University, Fredriksdal Museums and Gardens and in the gardens of Ystad Monastery. The microfauna of Betony at the four spontaneous localities was also investigated within the project. Adjacent to the project area, Holy-grass (Hierochlöe odorata (L.) P. Beauv.) and Upright Bugle (Ajuga genevensis L.) were established in the storm-water pond Vetedammen in Stehag. Some of the sponta- neous localities have been improved by grass and shrub clearance and, in one case, planting of local material. Overgrowth is by far the largest threat to these threatened species.
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| 7. |
- Yuh, Esther L, et al.
(author)
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Pathological computed tomography features associated with adverse outcomes after mild traumatic brain injury : A TRACK-TBI study with external validation in CENTER-TBI.
- 2021
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In: JAMA Neurology. - : American Medical Association (AMA). - 2168-6149 .- 2168-6157. ; 78:9, s. 1137-1148
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Journal article (peer-reviewed)abstract
- IMPORTANCE: A head computed tomography (CT) with positive results for acute intracranial hemorrhage is the gold-standard diagnostic biomarker for acute traumatic brain injury (TBI). In moderate to severe TBI (Glasgow Coma Scale [GCS] scores 3-12), some CT features have been shown to be associated with outcomes. In mild TBI (mTBI; GCS scores 13-15), distribution and co-occurrence of pathological CT features and their prognostic importance are not well understood.OBJECTIVE: To identify pathological CT features associated with adverse outcomes after mTBI.DESIGN, SETTING, AND PARTICIPANTS: The longitudinal, observational Transforming Research and Clinical Knowledge in Traumatic Brain Injury (TRACK-TBI) study enrolled patients with TBI, including those 17 years and older with GCS scores of 13 to 15 who presented to emergency departments at 18 US level 1 trauma centers between February 26, 2014, and August 8, 2018, and underwent head CT imaging within 24 hours of TBI. Evaluations of CT imaging used TBI Common Data Elements. Glasgow Outcome Scale-Extended (GOSE) scores were assessed at 2 weeks and 3, 6, and 12 months postinjury. External validation of results was performed via the Collaborative European NeuroTrauma Effectiveness Research in Traumatic Brain Injury (CENTER-TBI) study. Data analyses were completed from February 2020 to February 2021.EXPOSURES: Acute nonpenetrating head trauma.MAIN OUTCOMES AND MEASURES: Frequency, co-occurrence, and clustering of CT features; incomplete recovery (GOSE scores <8 vs 8); and an unfavorable outcome (GOSE scores <5 vs ≥5) at 2 weeks and 3, 6, and 12 months.RESULTS: In 1935 patients with mTBI (mean [SD] age, 41.5 [17.6] years; 1286 men [66.5%]) in the TRACK-TBI cohort and 2594 patients with mTBI (mean [SD] age, 51.8 [20.3] years; 1658 men [63.9%]) in an external validation cohort, hierarchical cluster analysis identified 3 major clusters of CT features: contusion, subarachnoid hemorrhage, and/or subdural hematoma; intraventricular and/or petechial hemorrhage; and epidural hematoma. Contusion, subarachnoid hemorrhage, and/or subdural hematoma features were associated with incomplete recovery (odds ratios [ORs] for GOSE scores <8 at 1 year: TRACK-TBI, 1.80 [95% CI, 1.39-2.33]; CENTER-TBI, 2.73 [95% CI, 2.18-3.41]) and greater degrees of unfavorable outcomes (ORs for GOSE scores <5 at 1 year: TRACK-TBI, 3.23 [95% CI, 1.59-6.58]; CENTER-TBI, 1.68 [95% CI, 1.13-2.49]) out to 12 months after injury, but epidural hematoma was not. Intraventricular and/or petechial hemorrhage was associated with greater degrees of unfavorable outcomes up to 12 months after injury (eg, OR for GOSE scores <5 at 1 year in TRACK-TBI: 3.47 [95% CI, 1.66-7.26]). Some CT features were more strongly associated with outcomes than previously validated variables (eg, ORs for GOSE scores <5 at 1 year in TRACK-TBI: neuropsychiatric history, 1.43 [95% CI .98-2.10] vs contusion, subarachnoid hemorrhage, and/or subdural hematoma, 3.23 [95% CI 1.59-6.58]). Findings were externally validated in 2594 patients with mTBI enrolled in the CENTER-TBI study.CONCLUSIONS AND RELEVANCE: In this study, pathological CT features carried different prognostic implications after mTBI to 1 year postinjury. Some patterns of injury were associated with worse outcomes than others. These results support that patients with mTBI and these CT features need TBI-specific education and systematic follow-up.
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