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| 2. |
- Klionsky, Daniel J., et al.
(author)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Research review (peer-reviewed)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Liang, Xifeng, et al.
(author)
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On the aerodynamic loads when a high speed train passes under an overhead bridge
- 2020
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In: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 202
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Journal article (peer-reviewed)abstract
- The aerodynamic loads on the overhead bridge bottom surface induced by train passage are reported in this paper. Both moving model test and numerical simulation approaches at the 1:20 scale are used. The numerical work is validated through both mesh independence tests and comparison with experimental data. Typical pressure variation curves are plotted and compared with previous studies. The peak pressure values’ dependence on the Reynolds number is considered through four sets of experiments with different train running speeds. The peak pressure coefficient distribution law for the bridge bottom surface is presented. Differences in the pressure distribution in different bridge bottom areas are explained based on more detailed flow field information. The influence of the bridge height on the aerodynamic load magnitude and time interval is presented. Moreover, the application of the CEN Standard to practical engineering issues is discussed.
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| 4. |
- Zhang, Ye, et al.
(author)
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A regularization method for the reconstruction of adsorption isotherms in liquid chromatography
- 2016
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In: Inverse Problems. - : IOP PUBLISHING LTD. - 0266-5611 .- 1361-6420. ; 32:10
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Journal article (peer-reviewed)abstract
- Determining competitive adsorption isotherms is an open problem in liquid chromatography. Since traditional experimental trial-and-error approaches are too complex and expensive, a modern technique of obtaining adsorption isotherms is to solve the inverse problem so that the simulated batch separation coincides with actual experimental results. This is a typical ill-posed problem. Moreover, in almost all cases the observed concentration at the outlet is the total response of all components, which makes the problem more difficult. In this work, we tackle the ill-posedness with a new regularization method, which is based on the fact that the adsorption isotherms do not depend on the injection profile. The proposed method transfers the original problem to an optimization problem with a time-dependent convection-diffusion equation constraint. Iterative algorithms for solving constraint optimization problems for both the equilibrium-dispersive and the transport-dispersive models are developed. The mass transfer resistance is also estimated by the proposed inverse method. A regularization parameter selection method and the convergence property of the proposed algorithm are discussed. Finally, numerical tests for both synthetic problems and real-world problems are given to show the efficiency and feasibility of the proposed regularization method.
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| 5. |
- Bentham, James, et al.
(author)
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A century of trends in adult human height
- 2016
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In: eLIFE. - 2050-084X. ; 5
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Journal article (peer-reviewed)abstract
- Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in South Korean women and Iranian men, who became 20.2 cm (95% credible interval 17.522.7) and 16.5 cm (13.319.7) taller, respectively. In contrast, there was little change in adult height in some sub-Saharan African countries and in South Asia over the century of analysis. The tallest people over these 100 years are men born in the Netherlands in the last quarter of 20th century, whose average heights surpassed 182.5 cm, and the shortest were women born in Guatemala in 1896 (140.3 cm; 135.8144.8). The height differential between the tallest and shortest populations was 19-20 cm a century ago, and has remained the same for women and increased for men a century later despite substantial changes in the ranking of countries.
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| 6. |
- Bentham, James, et al.
(author)
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A century of trends in adult human height
- 2016
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In: eLIFE. - : eLife Sciences Publications Ltd. - 2050-084X. ; 5
-
Journal article (peer-reviewed)abstract
- Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in South Korean women and Iranian men, who became 20.2 cm (95% credible interval 17.5–22.7) and 16.5 cm (13.3– 19.7) taller, respectively. In contrast, there was little change in adult height in some sub-Saharan African countries and in South Asia over the century of analysis. The tallest people over these 100 years are men born in the Netherlands in the last quarter of 20th century, whose average heights surpassed 182.5 cm, and the shortest were women born in Guatemala in 1896 (140.3 cm; 135.8– 144.8). The height differential between the tallest and shortest populations was 19-20 cm a century ago, and has remained the same for women and increased for men a century later despite substantial changes in the ranking of countries.
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| 7. |
- Chen, Guang, et al.
(author)
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Dynamic analysis of the effect of nose length on train aerodynamic performance
- 2019
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In: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 184, s. 198-208
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Journal article (peer-reviewed)abstract
- The improved delayed detached eddy simulation (IDDES) was used to study the influence of the train’s nose length on its aerodynamic performance. Both the time-averaged and instantaneous near-wake structures and the associated distribution of slipstream velocity are compared for three nose lengths. As the nose length increases, the mean and Std values of the drag and lift force are decreased. The shorter nose-length case results in a higher slipstream velocity. In particular, at the TSI track-side position, the TSI value U_2δ for the 5-m nose length case is 30% and 32% higher than the corresponding values for the 7.5-m and 10-m nose length cases, respectively. The dynamical flow topology in the wake reveals that the flow structures of the 5-m nose length are different from those of the other two cases in the tail streamline surface. As nose length increases, the longitudinal vortices are weaker, and the angle and distance between the longitudinal vortices are smaller. The shear production from the P_xy caused by the separation of the boundary layer at the lateral wall of the tail train is greater than that of the P_xz caused by the separation of the boundary layer at the top and bottom of the tail train.
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| 8. |
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| 9. |
- Fan, Mei-Cen, et al.
(author)
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Room-temperature extraction of individual elements from charged spent LiFePO4 batteries
- 2022
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In: Rare Metals. - : Springer. - 1001-0521 .- 1867-7185.
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Journal article (peer-reviewed)abstract
- Recycling millions of metric tons of spent LiFePO4 batteries would benefit human health while reducing resource depletion and environmental pollution. However, recovering individual elements from the spent batteries without generating waste is challenging. Here, we present a distinctive approach for recycling spent LiFePO4 batteries at room temperature, where water is the only leaching agent consumed. FePO4 and lithium intercalated graphite act as a precursor material for selectively extracting lithium, iron, and phosphorus through charging the LiFePO4 batteries to the delithiated state. NaOH solution extracted Fe from FePO4 within 30 min and regenerated without consumption, similar to a catalyst. Under the optimal leaching conditions (1 mol·L−1 NaOH, 0.5 h, NaOH/Fe molar ratio of 4.5), Fe and P leaching efficiencies achieved 89.1% and 99.2%, respectively. The methodology reflected in this research reduced the material cost per kg cathode material to a fraction of previously published reports, only occupies 6.13% of previous reports. In addition, the method improved the battery recycling revenue calculated by the EverBatt model by 2.31 times and 1.94 times over pyrometallurgical and hydrometallurgical methods. The proposed method allows for the convenient recovery of the elemental components of spent LiFePO4 batteries.
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| 10. |
- Guang, Liang, et al.
(author)
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A review of dynamic power management methods in NoC under emerging design considerations
- 2009
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In: 2009 NORCHIP. ; , s. 1-6
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Conference paper (peer-reviewed)abstract
- A review of dynamic and adaptive techniques for power management of on-chip interconnects, under emerging design considerations, is presented. The progress of IC technology has introduced novel methods, architectures and new challenges for power-aware design exploration. An examination of stateof-the-art power management techniques enables feasible and efficient design of future NoC platforms. This review first analyzes the new challenges, architectures and technologies, including PVT (process, voltage, temperature) variations, rapidly increasing leakage power, multiple on-chip PDN (power delivery network) as well as other architectures, which bring new considerations in low-power design exploration. A wide selection of dynamic power-saving techniques for onchip interconnects are examined, classified into several categories including run-time datapath configuration, supply configuration and adaptive encoding. The effects and feasibility of these methods, especially their potentials in future technology, are judiciously analyzed. An outlook on generic power management paradigms in next-generation NoCs concludes the review.
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