| 1. |
- Christmas, Matthew, et al.
(author)
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Evolutionary constraint and innovation across hundreds of placental mammals
- 2023
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In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 380:6643
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Journal article (peer-reviewed)abstract
- Zoonomia is the largest comparative genomics resource for mammals produced to date. By aligning genomes for 240 species, we identify bases that, when mutated, are likely to affect fitness and alter disease risk. At least 332 million bases (similar to 10.7%) in the human genome are unusually conserved across species (evolutionarily constrained) relative to neutrally evolving repeats, and 4552 ultraconserved elements are nearly perfectly conserved. Of 101 million significantly constrained single bases, 80% are outside protein-coding exons and half have no functional annotations in the Encyclopedia of DNA Elements (ENCODE) resource. Changes in genes and regulatory elements are associated with exceptional mammalian traits, such as hibernation, that could inform therapeutic development. Earth's vast and imperiled biodiversity offers distinctive power for identifying genetic variants that affect genome function and organismal phenotypes.
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| 2. |
- Clark, Andrew G., et al.
(author)
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Evolution of genes and genomes on the Drosophila phylogeny
- 2007
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In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 450:7167, s. 203-218
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Journal article (peer-reviewed)abstract
- Comparative analysis of multiple genomes in a phylogenetic framework dramatically improves the precision and sensitivity of evolutionary inference, producing more robust results than single-genome analyses can provide. The genomes of 12 Drosophila species, ten of which are presented here for the first time (sechellia, simulans, yakuba, erecta, ananassae, persimilis, willistoni, mojavensis, virilis and grimshawi), illustrate how rates and patterns of sequence divergence across taxa can illuminate evolutionary processes on a genomic scale. These genome sequences augment the formidable genetic tools that have made Drosophila melanogaster a pre-eminent model for animal genetics, and will further catalyse fundamental research on mechanisms of development, cell biology, genetics, disease, neurobiology, behaviour, physiology and evolution. Despite remarkable similarities among these Drosophila species, we identified many putatively non-neutral changes in protein-coding genes, non-coding RNA genes, and cis-regulatory regions. These may prove to underlie differences in the ecology and behaviour of these diverse species.
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| 3. |
- Kuderna, Lukas F. K., et al.
(author)
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Identification of constrained sequence elements across 239 primate genomes
- 2024
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In: Nature. - : Springer Nature. - 0028-0836 .- 1476-4687. ; 625:7996, s. 735-742
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Journal article (peer-reviewed)abstract
- Noncoding DNA is central to our understanding of human gene regulation and complex diseases1,2, and measuring the evolutionary sequence constraint can establish the functional relevance of putative regulatory elements in the human genome3,4,5,6,7,8,9. Identifying the genomic elements that have become constrained specifically in primates has been hampered by the faster evolution of noncoding DNA compared to protein-coding DNA10, the relatively short timescales separating primate species11, and the previously limited availability of whole-genome sequences12. Here we construct a whole-genome alignment of 239 species, representing nearly half of all extant species in the primate order. Using this resource, we identified human regulatory elements that are under selective constraint across primates and other mammals at a 5% false discovery rate. We detected 111,318 DNase I hypersensitivity sites and 267,410 transcription factor binding sites that are constrained specifically in primates but not across other placental mammals and validate their cis-regulatory effects on gene expression. These regulatory elements are enriched for human genetic variants that affect gene expression and complex traits and diseases. Our results highlight the important role of recent evolution in regulatory sequence elements differentiating primates, including humans, from other placental mammals.
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| 4. |
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| 5. |
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| 6. |
- Lindblad, Amanda, et al.
(author)
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Preferences, needs and attitudes regarding food habits and meal patterns among community-dwelling older adults : a cross-sectional survey
- 2017
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In: Exploring Future Foodscapes. - : AAU Captive Food Studies Group. - 9788797046203
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Conference paper (peer-reviewed)abstract
- The aim of this study is to explore preferences, needs and attitudes regarding food habits and meal patterns among community-dwelling older adults. 164 community-dwelling, 87-100-years old, 73 % women. Cross-sectional questionnaires based survey distributed by postal service. Approved by the Regional Ethical Review board in Gothenburg T231-14, addition to Dnr ref nr: 650-07. Results were, meanage 90.6 ± 2.84 yrs, mean BMI 23.7 ± 3.70 kg/m2, ♀71, ♂31 had no medical difficulties. More women than men lived alone, ♀102 ♂21 (p<0.01). ♀75 ♂28 would not consider home delivered convenience meals and ♀101 ♂23 cooked alone. Top three important practicalities when buying or receiving convenience meals were easy-open package (♀64 ♂23), easy to get food out (♀34 ♂13) and easily read (♀31 ♂16). This very old population shows good health evident by nutritional status and meal patterns. Subject characteristics indicated better condition than average Swedish nonagenarian, a minority reporting medical difficulties. Very few would consider convenience meals home delivered and would never use the computer. The conclusion is that an majority of both sexes preferred and managed planning, acquiring and cooking food independently although gender differences were apparent with women taking a greater responsibility. A great proportion was interested in testing new flavors. An urgent need concerned accessible containers with readable labels showing the importance for industry to acknowledge needs of older adults and accordingly adapt packaging for this age group.
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| 7. |
- Lindblad, Amanda, et al.
(author)
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Preferences, needs and attitudes regarding food habits and meal patterns among community-dwelling older adults : a cross-sectional survey
- 2017
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Conference paper (other academic/artistic)abstract
- The aim of this study is to explore preferences, needs and attitudes regarding food habits and meal patterns among community-dwelling older adults. 164 community-dwelling, 87-100-years old, 73 % women. Cross-sectional questionnaires based survey distributed by postal service. Approved by the Regional Ethical Review board in Gothenburg T231-14, addition to Dnr ref nr: 650-07. Results were, meanage 90.6 ± 2.84 yrs, mean BMI 23.7 ± 3.70 kg/m2, ♀71, ♂31 had no medical difficulties. More women than men lived alone, ♀102 ♂21 (p<0.01). ♀75 ♂28 would not consider home delivered convenience meals and ♀101 ♂23 cooked alone. Top three important practicalities when buying or receiving convenience meals were easy-open package (♀64 ♂23), easy to get food out (♀34 ♂13) and easily read (♀31 ♂16). This very old population shows good health evident by nutritional status and meal patterns. Subject characteristics indicated better condition than average Swedish nonagenarian, a minority reporting medical difficulties. Very few would consider convenience meals home delivered and would never use the computer. The conclusion is that an majority of both sexes preferred and managed planning, acquiring and cooking food independently although gender differences were apparent with women taking a greater responsibility. A great proportion was interested in testing new flavors. An urgent need concerned accessible containers with readable labels showing the importance for industry to acknowledge needs of older adults and accordingly adapt packaging for this age group.
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| 8. |
- Rothenberg, Elisabet, 1960-, et al.
(author)
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Body composition and hand grip strength in healthy community-dwelling older adults in sweden
- 2015
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In: Journal of Aging Research & Clinical Practice. - 2273-421X. ; 4:1, s. 54-58
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Journal article (peer-reviewed)abstract
- Background: Longevity increases worldwide but there are few studies on body composition and hand grip strength inpopulations over 80 years. Given high prevalence of chronic disease and functional disability in octogenarians, it may be difficult todistinguish effects of ageing from those imposed by disease. The European Consensus definition of sarcopenia recommends usingboth low muscle mass and function for diagnosis.Objectives: Examine body composition and hand grip strength in a selected groupof community-dwelling older adults with high level of functional independence. In addition, longitudinal changes in handgripstrength were examined using previously collected data.Design: Cross-sectional body composition and hand grip strength witha four year retrospective analysis on previously assessed hand grip strength.Setting: Measurements were conducted by homevisits.Participants: 102 community-dwelling 83-96 year-olds, 50 % women.Measurements: Hand grip strength was registered bya dynamometer and body composition analysis using bioimpedance spectroscopy.Results: According to European Consensusdefinition, only 6/102 had normal muscle mass - no men, although 78 % of men and 40 % of women had normal muscle strength.Since previously collected data four years earlier, men had lost strength (p<0.001), while women had not (p=0.202).Conclusions: Subject characteristics and health status support well-preserved body energy, protein stores and muscle strength. Low musclemass was much more prevalent than low muscle strength. Results may give an indication of what constitutes a healthy bodycomposition in oldest old and could serve as a starting point for reference values on healthy body composition in octogenarians.
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| 9. |
- Rothenberg, Elisabet, et al.
(author)
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Body composition and hand grip strength in healthy community-dwelling older adults in sweden
- 2015
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In: Journal of Aging Research & Clinical Practice. - : SERDI. - 2273-421X. ; 4:1, s. 54-58
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Journal article (peer-reviewed)abstract
- Background: Longevity increases worldwide but there are few studies on body composition and hand grip strength inpopulations over 80 years. Given high prevalence of chronic disease and functional disability in octogenarians, it may be difficult todistinguish effects of ageing from those imposed by disease. The European Consensus definition of sarcopenia recommends usingboth low muscle mass and function for diagnosis. Objectives: Examine body composition and hand grip strength in a selected groupof community-dwelling older adults with high level of functional independence. In addition, longitudinal changes in handgripstrength were examined using previously collected data. Design: Cross-sectional body composition and hand grip strength witha four year retrospective analysis on previously assessed hand grip strength. Setting: Measurements were conducted by homevisits. Participants: 102 community-dwelling 83-96 year-olds, 50 % women. Measurements: Hand grip strength was registered bya dynamometer and body composition analysis using bioimpedance spectroscopy. Results: According to European Consensusdefinition, only 6/102 had normal muscle mass - no men, although 78 % of men and 40 % of women had normal muscle strength.Since previously collected data four years earlier, men had lost strength (p<0.001), while women had not (p=0.202). Conclusions: Subject characteristics and health status support well-preserved body energy, protein stores and muscle strength. Low musclemass was much more prevalent than low muscle strength. Results may give an indication of what constitutes a healthy bodycomposition in oldest old and could serve as a starting point for reference values on healthy body composition in octogenarians.
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