| 1. |
- Cheng, Q., et al.
(author)
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Clinical epidemiology of Guillain-Barré syndrome in adults in Sweden 1996-97 : A prospective study
- 2000
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In: European Journal of Neurology. - : Wiley. - 1351-5101 .- 1468-1331. ; 7:6, s. 685-692
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Journal article (peer-reviewed)abstract
- We described clinical manifestations, outcomes, prognostic indicators and clinico-epidemiological subgroups for 53 adult patients with Guillain-Barré syndrome (GBS) in Sweden during the period 1996-97. These patients were identified from a population of 2.8 million inhabitants and prospectively followed up for one year by a network of neurologists. An additional 10 cases, of whom five were adults who had not been prospectively followed up, were not included in the analyses. At 6 months after onset 80% of the patients could walk without aid, while at 1 year 46% were fully recovered, 42% had mild residual signs or symptoms, 4% had moderate and 6% severe disabilities, and 2% had died. Intravenous human immunoglobulin or plasmapheresis were used in 72% of the patients. The sum of the Medical Research Council (MRC) score at nadir was found as the only significant predictor for residual signs at 1 year in a multivariate model. Three subgroups, with different clinico-epidemiological characteristics, were identified by using cluster analysis. In conclusion, GBS in Sweden is frequently preceded by a respiratory infection, is often treated with immunomodulatory therapies, and exhibits a high recovery rate and a low fatality rate.
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| 2. |
- Konold, Patrick E., et al.
(author)
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Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
- 2024
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In: NATURE METHODS. - : NATURE PORTFOLIO. - 1548-7091 .- 1548-7105.
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Journal article (peer-reviewed)abstract
- Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining J alpha helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes. The MHz repetition rates available at second-generation X-ray free-electron lasers enable the collection of microsecond time-resolved X-ray scattering data with exceptionally low noise, providing insights into protein structural dynamics.
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| 3. |
- Lindsten, K, et al.
(author)
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GFP reporter mouse models of UPS proteolytic function
- 2006
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In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. - : Wiley. - 1530-6860. ; 20:7, s. 1027-author
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Journal article (other academic/artistic)
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| 4. |
- Sanga, M., et al.
(author)
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A panel of PCR-inhibitory reference materials for quality evaluation of multiplex STR analysis kits : -
- 2015
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In: Abstracts ISFG. ; , s. 226-
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Conference paper (peer-reviewed)abstract
- PCR inhibition is a key challenge in forensic DNA analysis. Substances interfering with the amplification of PCR products can lower the success rate of Short Tandem Repeat (STR) analysis or generate ambiguous DNA profiles.For forensic DNA laboratories it is therefore vital to have knowledge about how common inhibitory substances affect their STR analysis system. We have developed a broad-range panel of standardized PCR-inhibitory reference materials (RMs), representing the heterogeneous stains found at crime scenes. The panel, including solutions prepared from for example cigarette paper,chewing gum, moist snuff and humic acid, is a tool for quality evaluation of STR systems. We applied the RMs to challenge PowerPlex® ESX 16 Fast System (ESX Fast). Although ESX Fast tolerated high levels of some inhibitors, several RMs caused problems in different ways. Humic acid had a specific negative effect on amelogenin amplification, moist snuff hindered amplification of longer fragments, and chewing gum caused generally lowered allele peak heights. These different effects may provide information regarding the mechanisms of inhibition. For instance, our results indicate that one effect of humic acid on ESX Fast analysis is chelation of Mg2+ ions, thus altering the melting temperatures of the primers. In the developmental validation of STR systems, a limited evaluation of PCR inhibition involving only a few substances is generally performed. Applying a broad panel of RMs will ensure that a wider range of inhibitory substances from crime scene samples are included, giving a better understanding of inhibitor tolerance and effects.
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