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  • Thomas, HS, et al. (author)
  • 2019
  • swepub:Mat__t
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  • Beal, Jacob, et al. (author)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Journal article (peer-reviewed)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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  • Result 1-10 of 783
Type of publication
journal article (626)
research review (17)
conference paper (6)
other publication (1)
Type of content
peer-reviewed (761)
other academic/artistic (9)
Author/Editor
Liu, Y. (504)
Jones, G. (491)
Milov, A. (490)
Esposito, B. (485)
Jinnouchi, O. (481)
Arai, Y. (475)
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Jain, V. (472)
Hill, J. C. (470)
Li, H. (469)
Moore, R. W. (469)
Chen, S. (468)
Kolanoski, H. (468)
Chen, H. (467)
Francis, D. (467)
King, M. (467)
Losada, M. (467)
Martin, B. (467)
Davies, M. (466)
Ferrer, A. (466)
Antonelli, M. (465)
Barklow, T. (465)
Bauer, F. (465)
Bella, G. (465)
Benekos, N. (465)
Blumenschein, U. (465)
Boonekamp, M. (465)
Calderini, G. (465)
Chen, X. (465)
Cranmer, K. (465)
Dallapiccola, C. (465)
Eigen, G. (465)
Etzion, E. (465)
Fassouliotis, D. (465)
Fleck, I. (465)
Gross, E. (465)
Hamacher, K. (465)
Hughes, G. (465)
Kawagoe, K. (465)
Kawamoto, T. (465)
Kim, S. H. (465)
Kobayashi, T. (465)
Kourkoumelis, C. (465)
Liebig, W. (465)
Lucotte, A. (465)
Macchiolo, A. (465)
Martinez, M. (465)
Meyer, J. (465)
Mohr, W. (465)
Morii, M. (465)
Nagai, K. (465)
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University
Lund University (417)
Uppsala University (411)
Royal Institute of Technology (329)
Stockholm University (318)
Karolinska Institutet (175)
Chalmers University of Technology (50)
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University of Gothenburg (48)
Högskolan Dalarna (47)
Umeå University (39)
Linköping University (9)
Mid Sweden University (8)
Örebro University (6)
University of Skövde (6)
Södertörn University (5)
Swedish University of Agricultural Sciences (4)
Luleå University of Technology (2)
Halmstad University (1)
Jönköping University (1)
Stockholm School of Economics (1)
The Swedish School of Sport and Health Sciences (1)
Linnaeus University (1)
IVL Swedish Environmental Research Institute (1)
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Language
English (783)
Research subject (UKÄ/SCB)
Natural sciences (429)
Medical and Health Sciences (127)
Engineering and Technology (23)
Social Sciences (9)
Agricultural Sciences (2)
Humanities (1)

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