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- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2. |
- Liu, Lindong, et al.
(author)
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Intelligent simulation experimental study on influence of air velocity of air supply hood and exhaust hood with vertical push-pull ventilation
- 2019
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In: Journal of Intelligent & Fuzzy Systems. - : IOS PRESS. - 1064-1246 .- 1875-8967. ; 37:4, s. 4819-4826
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Journal article (peer-reviewed)abstract
- The occupational health and safety problems caused by indoor dust and toxic gases in industrial plants are becoming increasingly serious. Vertical push-pull ventilation can effectively control dust and toxic gases and avoid indoor environmental pollution. The velocity of the air supply hood and exhaust hood in the push-pull ventilation intelligent device directly affects the control effect of the dust and toxic gases. However, no scholar has given a reasonable ratio of the air supply and exhaust velocity of the vertical push-pull ventilation device. Therefore, this paper uses the combination of Fluent numerical simulation technology and smoke visualization experiment to explore the best ratio of the air supply and exhaust velocity in the push-pull ventilation flow field. The research shows that the traces of the wind flow obtained by numerical analysis under different k which is the ratio of the air supply and exhaust velocity is consistent with the traces of visualized smoke in the actual experiment; The optimal ratio of air supply and exhaust is k = 1.75, which can effectively guarantee the indoor environment, occupational health and work efficiency of the operators.
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