| 1. |
- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Wang, Mengmeng, et al.
(author)
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Statistical analysis of whistler precursors upstream of foreshock transient shocks : MMS observations
- 2024
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In: Geophysical Research Letters. - : American Geophysical Union (AGU). - 0094-8276 .- 1944-8007. ; 51:8
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Journal article (peer-reviewed)abstract
- Using the high-time-resolution data from the Magnetospheric Multiscale mission, precursor waves upstream of foreshock transient (FT) shocks are statistically investigated using the four-spacecraft timing method. The wave frequencies and wave vectors determined in the plasma rest frame (PRF) are shown to follow the cold plasma dispersion relation for whistler waves. Combining with the feature of the right-hand polarization in the PRF, the precursors are identified as whistler-mode waves around the lower hybrid frequency. The occurrence of whistler precursors is independent of the Alfvén Mach number and the FT shock normal angle. More importantly, all the whistler precursors have group velocities pointing upstream in the shock frame, suggesting the dispersive radiation to be a possible generation mechanism. The study improves the understanding of not only the whistler precursors but also the overall FT shock dynamics.
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| 3. |
- Liu, Yuqi, et al.
(author)
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Digital Twins of Wave Energy Generation Based on Artificial Intelligence
- 2022
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In: 2022 IEEE Conference on Virtual Reality and 3D User Interfaces Abstracts and Workshops (VRW). - : Institute of Electrical and Electronics Engineers (IEEE). - 9781665484022 - 9781665484039 ; , s. 718-719
-
Conference paper (peer-reviewed)abstract
- Ocean waves provide a large amount of renewable energy, and Wave energy converter (WEC) can convert wave energy into electric energy. This paper proposes a visualization platform for wave power generation. The platform can monitor various indicators of wave power generation in real time, combined with Long Short-Term Memory (LSTM) neural network to predict wave power and electricity consumption. We make digital twins of a wave power plant in a computer, allowing users to remotely view the factory through VR glasses.
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| 4. |
- Liu, Yuqi, et al.
(author)
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Integration of Multi-scale Spatial Digital Twins in Metaverse Based on Multi-dimensional Hash Geocoding
- 2024
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In: IMX '24. - : Association for Computing Machinery (ACM). - 9798400705038 ; , s. 56-63
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Conference paper (peer-reviewed)abstract
- With the popularization of the metaverse, virtual reality mapping technology based on digital twins has generated a large amount of spatial data. These data are multidimensional, multi-scale, mobile, and distributed. In order to fully utilize these data, we propose a non mutation multidimensional hash geocoding that can organize and store data with geographic features, and achieve data mapping at different scales from macro to micro. The mapping between them can achieve joint utilization of data of various scales. On this basis, we propose a block network secure storage mapping model for spatial digital twins, which can securely and reliably organize and map spatial data. This article also looks forward to the possible emergence of digital twins of different dimensions and scales in the future metaverse, and proposes an adaptive 3D reconstruction method based on this to adapt to digital twins models of different scales in the metaverse. On the basis of our work, we will further promote the development of the spatial digital twin metaverse.
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| 5. |
- Liu, Yuqi, et al.
(author)
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Liquid Digital Twins Based on Magnetic Fluid Toys
- 2022
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In: 2022 IEEE Conference on Virtual Reality and 3D User Interfaces Abstracts and Workshops (VRW). - : IEEE. - 9781665484022 ; , s. 988-989
-
Conference paper (peer-reviewed)abstract
- As a new type of functional material, magnetic fluid has both the fluidity of liquid and the magnetic properties of solid magnetic material. By controlling the magnets, one can simulate the effect of manipulating liquids like a sea emperor. This will provide new ideas for the multiverse of the metaverse. Not only that, magnetic fluids also have very important applications in astrophysics, controlled thermonuclear reactions and even the medical industry. Therefore, this paper hopes to provide a control idea for the future application of magnetic fluid by performing Digital Twins simulation of magnetic fluid.
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| 6. |
- Long, Tao, et al.
(author)
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Constraining the formation and transport of lunar impact glasses using the ages and chemical compositions of Chang’e-5 glass beads
- 2022
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In: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 8:39
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Journal article (peer-reviewed)abstract
- Impact glasses found in lunar soils provide a possible window into the impact history of the inner solar system. However, their use for precise reconstruction of this history is limited by an incomplete understanding of the physical mechanisms responsible for their origin and distribution and possible relationships to local and regional geology. Here, we report U-Pb isotopic dates and chemical compositions of impact glasses from the Chang’e-5 soil and quantitative models of impact melt formation and ejection that account for the compositions of these glasses. The predominantly local provenance indicated by their compositions, which constrains transport distances to <~150 kilometers, and the age-frequency distribution are consistent with formation mainly in impact craters 1 to 5 kilometers in diameter. Based on geological mapping and impact cratering theory, we tentatively identify specific craters on the basaltic unit sampled by Chang’e-5 that may have produced these glasses and compare their ages with the impact record of the asteroid belt.
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| 7. |
- Bian, Zengxue, et al.
(author)
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The Digital Twins of Thor's Hammer Based on Motion Sensing
- 2022
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In: 2022 IEEE Conference on Virtual Reality and 3D User Interfaces Abstracts and Workshops (VRW). - : IEEE. - 9781665484022 ; , s. 894-895
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Conference paper (peer-reviewed)abstract
- Ancient humans attribute the phenomenon of thunder and lightning to divine power. The power of Thor that can lift Thor's Hammer, the body not be hurt by thunder and lightning. It's not impossible for us to control thunder and lightning like Thor. The Digital Twins system of the robotic arm designed in this paper integrates the physical device of the robotic arm, the digital model of robotic arm, the body sense interaction, and the virtual-reality mapping module. It can digitally control the robotic arm. With this system, we can all lift Thor's hammer in the future.
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| 8. |
- Hu, Yuning, et al.
(author)
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Cooking methods affect the intake of per- and polyfluoroalkyl substances (PFASs) from grass carp
- 2020
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In: Ecotoxicology and Environmental Safety. - : Academia Press. - 0147-6513 .- 1090-2414. ; 203
-
Journal article (peer-reviewed)abstract
- Consumption of fish is one of the predominant sources of human exposure to per- and polyfluoroalkyl substances (PFASs). In this study, the effect of various cooking methods (boiling, steaming, grilling and frying) on the levels of PFASs in fish muscle and the intake of PFASs was explored by using grass carp collected from Tangxun Lake, Wuhan, China. Perfluorooctane sulfonate (PFOS) was the predominant PFASs in raw fish fillets, with the concentrations ranging from 59.6 to 136 ng/g ww, followed by perfluorobutane sulfonate (PFBS) (7.73-51.9 ng/g ww). The concentrations of long-chain PFASs in fish increased after cooking, while those of short-chain PFASs decreased. The amounts of PFASs in the cooked fish fillets decreased except PFOS. Short-chain PFASs, including PFBS and perfluoroheptanoic acid (PFHpA), were dominant in cooking juice. The highest amounts of PFBS in the juices were observed after boiling and frying, even higher than those in cooked fish fillets, suggesting that the release of short-chain PFASs to the cooking juices could not be neglected. Based on these results, the intake of short-chain PFASs amount through cooked fish fillets slightly decreased, but the intake of PFOS amount increased. However, consumption of cooking juice (fish soup) could increase the exposure risk of PFBS. Comprehensively considering the increase of PFOS and decrease of total PFASs, boiling may be the relatively better method to cook fish. As PFASs are ubiquitous and inevitable in aquatic food, it is thus important to choose appropriate cooking processes and dietary habits for reducing the intake of different PFASs from fish.
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