| 1. |
- Cruz, Raquel, et al.
(author)
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Novel genes and sex differences in COVID-19 severity
- 2022
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In: Human Molecular Genetics. - : Oxford University Press. - 0964-6906 .- 1460-2083. ; 31:22, s. 3789-3806
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Journal article (peer-reviewed)abstract
- Here, we describe the results of a genome-wide study conducted in 11 939 coronavirus disease 2019 (COVID-19) positive cases with an extensive clinical information that were recruited from 34 hospitals across Spain (SCOURGE consortium). In sex-disaggregated genome-wide association studies for COVID-19 hospitalization, genome-wide significance (P < 5 × 10−8) was crossed for variants in 3p21.31 and 21q22.11 loci only among males (P = 1.3 × 10−22 and P = 8.1 × 10−12, respectively), and for variants in 9q21.32 near TLE1 only among females (P = 4.4 × 10−8). In a second phase, results were combined with an independent Spanish cohort (1598 COVID-19 cases and 1068 population controls), revealing in the overall analysis two novel risk loci in 9p13.3 and 19q13.12, with fine-mapping prioritized variants functionally associated with AQP3 (P = 2.7 × 10−8) and ARHGAP33 (P = 1.3 × 10−8), respectively. The meta-analysis of both phases with four European studies stratified by sex from the Host Genetics Initiative (HGI) confirmed the association of the 3p21.31 and 21q22.11 loci predominantly in males and replicated a recently reported variant in 11p13 (ELF5, P = 4.1 × 10−8). Six of the COVID-19 HGI discovered loci were replicated and an HGI-based genetic risk score predicted the severity strata in SCOURGE. We also found more SNP-heritability and larger heritability differences by age (<60 or ≥60 years) among males than among females. Parallel genome-wide screening of inbreeding depression in SCOURGE also showed an effect of homozygosity in COVID-19 hospitalization and severity and this effect was stronger among older males. In summary, new candidate genes for COVID-19 severity and evidence supporting genetic disparities among sexes are provided.
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| 2. |
- Macias, Raul, et al.
(author)
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Performance of the Load-in-the-Loop Single Op-Amp Voltage Controlled Current Source from the Op-Amp Parameters
- 2010
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In: Journal of Physics: Conference Series. - : Institute of Physics Publishing Ltd.. - 1742-6596 .- 1742-6588.
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Conference paper (peer-reviewed)abstract
- In recent years, Electrical Bioimpedance (EBI) methods have gained importance. These methods are often based on obtaining impedance spectrum in the range of β-dispersion, i.e. from a few kHz up to some MHz. To measure EBI a constant current is often injected and the voltage across the tissue under study (TUS) is recorded. Due to the performance of the current source influences the performance of the entire system, in terms of frequency range, several designs have been implemented and studied. In this paper the basic structure of a Voltage-Controlled Current Source (VCCS) based on a single Op-Amp in inverter configuration with a floating load, known as load-in-the-loop current source, is revisited and studied deeply. We focus on the dependence of the output impedance with the circuit parameters, i.e. the feedback resistor and the inverter-input resistor, and the Op-Amp main parameters, i.e. open loop gain, CMRR and input impedance. After obtaining the experimental results and comparing to the theoretical and simulated ones, they confirm the design under study can be a good solution for multi-frequency wideband EBI applications because of higher values of the output impedance than 100kΩ at 1MHz are obtained. Furthermore, an enhancement of the basic design, using a current conveyor as a first stage, is proposed, studied and implemented.
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| 3. |
- Osorio-Macías, Daniel E., et al.
(author)
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Characterization on the impact of different clarifiers on the white wine colloids using Asymmetrical Flow Field-Flow Fractionation
- 2022
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In: Food Chemistry. - : Elsevier BV. - 0308-8146. ; 381
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Journal article (peer-reviewed)abstract
- Clarifiers are substances used during the winemaking process to enhance clarity and stability in the wines. The different clarifiers may alter removal capacities differently. In this study, the removal efficiency of seven common fining agents, divided into three groups (mineral clarifiers, synthetic polymeric clarifiers, and vegetable protein clarifiers), was analyzed with Asymmetrical Flow Field-Flow fractionation (AF4). Besides, the relationship between the removal capacity and different molecular and macromolecular properties has been evaluated. The results showed extensive removal of colloidal and macromolecular matter by the bentonites with potential impact on characteristic properties of the wine. The vegetable clarifiers showed a more profiled reduction, potentially preserving characteristics of the wine. The synthetic polymers showed a more limited removal efficiency but with a high affinity to remove colloidal phenols. The use of AF4-UV-MALS-dRI allowed the characterization of the wines after different clarification treatments, showing to be an analytical technique with a potential impact on the wine industry.
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| 4. |
- Osorio-Macías, Daniel E., et al.
(author)
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Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation
- 2020
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In: Journal of Agricultural and Food Chemistry. - : American Chemical Society (ACS). - 0021-8561 .- 1520-5118. ; 68:49, s. 14564-14576
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Journal article (peer-reviewed)abstract
- The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (AsFlFFF−MALS−dRI−UV) for the fractionation and determination of the molar mass, the hydrodynamic radius, and the apparent densities of the aggregates and macromolecules present in wine samples. The results from a set of six Argentinian high-altitude wines showed two main populations: the first population composed of wine colloids with higher UV-specific absorptivity and the second population composed of polysaccharides, such as arabinogalactans. The conformation results showed that population 1 consists of small and dense particles, while population 2 showed high molar masses and lower densities. The results demonstrated the use of AsFlFFF as a new, effective method for the fractionation and characterization of wine colloids and wine macromolecules in red wines with further potential applications.
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