| 1. |
- Heiss, Christian, et al.
(author)
-
The compelling arguments for the need of medical vascular physicians in Europe
- 2019
-
In: Vasa - European Journal of Vascular Medicine. - : Hogrefe Publishing Group. - 0301-1526. ; 48:6
-
Journal article (peer-reviewed)abstract
- The burden of vascular diseases is growing worldwide, as the population ages, prompting a call to action not only in terms of awareness but also and most urgently in recognizing the need for vascular physicians, also called angiologists. Vascular medicine views the vascular system (arteries, veins, and lymphatics) as a whole, unique, and independent entity requiring specialized competencies. Vascular physicians offer a holistic and comprehensive approach to vascular patients including provision of interventional procedures, management of a heterogeneous group of multi-morbid and frail patients affected by multi-vessel diseases, and connecting different specialists in a multidisciplinary effort. Vascular medicine practise varies across European countries. While it is a firmly accepted medical speciality in many European countries it is not formally recognized by the European Union limiting adoption in the other countries. The lack of vascular physicians likely accounts for inequality of care of vascular patients as compared for example to patients with heart disease and might contribute to adverse outcomes and healthcare costs associated with vascular diseases. To move forward in the struggle to provide efficient care for multimorbid poly-vascular patients, it is essential to establish vascular medicine programs in Europe and worldwide. Important steps to achieve this goal include improving public awareness of vascular diseases, attain formal recognition by the EU of angiology/vascular medicine as a medical specialty, creating specialized treatment guidelines, and to harmonize vascular care in Europe.
|
|
| 2. |
|
|
| 3. |
- Perfecto-Avalos, Yocanxóchitl, et al.
(author)
-
Denaturing high-performance liquid chromatography and principal component analysis for identification of DNA point mutations in breast cancer and lymphoma samples
- 2018
-
In: Journal of Chemometrics. - : Wiley. - 0886-9383. ; 32:10
-
Journal article (peer-reviewed)abstract
- Abstract DNA mutations are identified by techniques that use the knowledge of the wild-type DNA sequence and its mutated variant. The involved analytic methods must be accurate, rapid, and sustainable, if a clinical application is pursued. High-performance liquid chromatography under denaturing conditions is a useful technique to screen mutations. Denaturing high-performance liquid chromatography resultant chromatograms are suitable for feature extraction analysis with multivariate methods such as principal component analysis. In this work, principal component analysis was applied to analyze the chromatograms from 3 different genes. Fragments with verified wild-type sequence were used as reference and samples with sequence unknown were tested. A statistical characterization based on Tukey’s boxplot equation of principal component scores allowed us to analyze the distance distribution between reference and sample clusters to establish a classification criterion: an outlier could represent a mutated sample, and a typical value could be a wild-type sample. Identified outliers were further analyzed by sequencing and proved to carry a mutation. From 72 datasets with a total of 4258 injections, we successfully assessed the classification criterion, identifying mutated samples in lymphoma and breast cancer patients with ratio of prediction Gmean = [0.89, 1.00]. Compared with sequencing analysis, this procedure reduced time and costs.
|
|
