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  • Hillberg, Louise, et al. (author)
  • Tropomyosins are present in lamellipodia of motile cells
  • 2006
  • In: European Journal of Cell Biology. - : Elsevier BV. - 0171-9335 .- 1618-1298. ; 85:5, s. 399-409
  • Journal article (peer-reviewed)abstract
    • This paper shows that high-molecular-weight tropomyosins (TMs), as well as shorter isoforms of this protein, are present in significant amounts in lamellipodia and filopodia of spreading normal and transformed cells. The presence of TM in these locales was ascertained by staining of cells with antibodies reacting with endogenous TMs and through the expression of hemaglutinin- and green fluorescent protein-tagged TM isoforms. The observations are contrary to recent reports suggesting the absence of TMs in regions,where polymerization of actin takes place, and indicate that the view of the role of TM in the formation of actin filaments needs to be significantly revised.
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