| 1. |
- Andersson, Rebecca, et al.
(author)
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Differential role of cytosolic Hsp70s in longevity assurance and protein quality control
- 2021
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In: PLoS Genetics. - : Public Library of Science (PLoS). - 1553-7404 .- 1553-7390. ; 17:1
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Journal article (peer-reviewed)abstract
- 70 kDa heat shock proteins (Hsp70) are essential chaperones of the protein quality control network; vital for cellular fitness and longevity. The four cytosolic Hsp70's in yeast, Ssa1-4, are thought to be functionally redundant but the absence of Ssa1 and Ssa2 causes a severe reduction in cellular reproduction and accelerates replicative aging. In our efforts to identify which Hsp70 activities are most important for longevity assurance, we systematically investigated the capacity of Ssa4 to carry out the different activities performed by Ssa1/2 by overproducing Ssa4 in cells lacking these Hsp70 chaperones. We found that Ssa4, when overproduced in cells lacking Ssa1/2, rescued growth, mitigated aggregate formation, restored spatial deposition of aggregates into protein inclusions, and promoted protein degradation. In contrast, Ssa4 overproduction in the Hsp70 deficient cells failed to restore the recruitment of the disaggregase Hsp104 to misfolded/aggregated proteins, to fully restore clearance of protein aggregates, and to bring back the formation of the nucleolus-associated aggregation compartment. Exchanging the nucleotide-binding domain of Ssa4 with that of Ssa1 suppressed this 'defect' of Ssa4. Interestingly, Ssa4 overproduction extended the short lifespan of ssa1 Delta ssa2 Delta mutant cells to a lifespan comparable to, or even longer than, wild type cells, demonstrating that Hsp104-dependent aggregate clearance is not a prerequisite for longevity assurance in yeast. Author summary All organisms have proteins that network together to stabilize and protect the cell throughout its lifetime. One of these types of proteins are the Hsp70s (heat shock protein 70). Hsp70 proteins take part in folding other proteins to their functional form, untangling proteins from aggregates, organize aggregates inside the cell and ensure that damaged proteins are destroyed. In this study, we investigated three closely related Hsp70 proteins in yeast; Ssa1, 2 and 4, in an effort to describe the functional difference of Ssa4 compared to Ssa1 and 2 and to answer the question: What types of cellular stress protection are necessary to reach a normal lifespan? We show that Ssa4 can perform many of the same tasks as Ssa1 and 2, but Ssa4 doesn't interact in the same manner as Ssa1 and 2 with other types of proteins. This leads to a delay in removing protein aggregates created after heat stress. Ssa4 also cannot ensure that misfolded proteins aggregate correctly inside the nucleus of the cell. However, this turns out not to be necessary for yeast cells to achieve a full lifespan, which shows us that as long as cells can prevent aggregates from forming in the first place, they can reach a full lifespan.
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| 2. |
- Benselfelt, Tobias, et al.
(author)
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The Colloidal Properties of Nanocellulose
- 2023
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In: ChemSusChem. - : John Wiley and Sons Inc. - 1864-5631 .- 1864-564X. ; 16:8, s. e202201955-
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Journal article (peer-reviewed)abstract
- Nanocelluloses are anisotropic nanoparticles of semicrystalline assemblies of glucan polymers. They have great potential as renewable building blocks in the materials platform of a more sustainable society. As a result, the research on nanocellulose has grown exponentially over the last decades. To fully utilize the properties of nanocelluloses, a fundamental understanding of their colloidal behavior is necessary. As elongated particles with dimensions in a critical nanosize range, their colloidal properties are complex, with several behaviors not covered by classical theories. In this comprehensive Review, we describe the most prominent colloidal behaviors of nanocellulose by combining experimental data and theoretical descriptions. We discuss the preparation and characterization of nanocellulose dispersions, how they form networks at low concentrations, how classical theories cannot describe their behavior, and how they interact with other colloids. We then show examples of how scientists can use this fundamental knowledge to control the assembly of nanocellulose into new materials with exceptional properties. We hope aspiring and established researchers will use this Review as a guide. © 2023 The Authors.
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| 3. |
- Gallardo, Rodrigo, et al.
(author)
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De novo design of a biologically active amyloid
- 2016
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In: Science. - : AMER ASSOC ADVANCEMENT SCIENCE. - 0036-8075 .- 1095-9203. ; 354:6313, s. 720-
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Journal article (peer-reviewed)abstract
- Most human proteins possess amyloidogenic segments, but only about 30 are associated with amyloid-associated pathologies, and it remains unclear what determines amyloid toxicity. We designed vascin, a synthetic amyloid peptide, based on an amyloidogenic fragment of vascular endothelial growth factor receptor 2 (VEGFR2), a protein that is not associated to amyloidosis. Vascin recapitulates key biophysical and biochemical characteristics of natural amyloids, penetrates cells, and seeds the aggregation of VEGFR2 through direct interaction. We found that amyloid toxicity is observed only in cells that both express VEGFR2 and are dependent on VEGFR2 activity for survival. Thus, amyloid toxicity here appears to be both protein-specific and conditional-determined by VEGFR2 loss of function in a biological context in which target protein function is essential.
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| 4. |
- Karlsson, Tobias, 1987-, et al.
(author)
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A Path Towards a Possible Future – Adult Students’ Choice of Vocational Education
- 2022
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In: Vocations and Learning. - : Springer Netherlands. - 1874-785X .- 1874-7868. ; 15:1, s. 111-128
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Journal article (peer-reviewed)abstract
- Today’s society is characterized by high unemployment, a prevailing trust in and demands for an academic degree, and an emphasis on the individual’s own responsibility for their educational choices. This study aims to examine adults’ vocational education choices, their intentions in connection with municipal adult education (MAE) studies, and how this relates to identity formation. The study is based on 18 interviews and compares students from two vocational MAE training programmes in assistant nursing and floor laying. The analysis has identified different pathways concerning adult students’ decisions to enrol in municipal adult education and a specific vocational education and training (VET) programme. We see educational choices and paths in terms of underlying causes or as forward-looking rationalities. The results show that the process of identity formation is larger than simply one of vocational becoming within a vocational community of practice, since MAE studies involve a student’s whole being, including both their personal identity trajectories and their vocational identity formation. With this article we hope to provide a foundation for a pedagogical discussion about student intentions, focusing on how different subjectivities affect students with regard to their future vocational becoming.
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| 5. |
- Karlsson, Tobias Lasse, 1987-
(author)
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Something to Do Something to Be : A study of students' vocational identity formation in adult education
- 2023
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Doctoral thesis (other academic/artistic)abstract
- The aim of this thesis is to understand how vocations are learned in adult education by exploring the vocational identity formation of students in Municipal Adult Education (MAE) in Sweden. By analysing interviews of adult students during different stages in their Vocational Education and Training (VET) through working life, this study contributes to the fields of adult education and VET. The theoretical framework centres on the concept of identity, utilising communities of practice, and is nuanced with the concepts of sense of self and vocational habitus, in order to analyse both social practices and individual aspects of the students’ vocational identity formation.The most standout finding is that VET in adult education can provide something beyond forming a vocational identity. In essence, the process of successfully completing an education can improve how students perceive themselves as adults, learners, and active members in society. The findings also show how the intersection between students’ perceptions of themselves, their education, and their vocation create barriers that they must overcome in order to progress in their identity formation. Overcoming barriers is an important part of the learning process that is towards a vocational identity, however, while some barriers are surpassed, some persist, and some are substituted by new ones.These processes in turn affect how students identify and dis-identify with educational and vocational practices, which in turn influence how they participate in these practices. To the extent that students progress in their vocational identity depends on a variety of factors, related to their ability to participate in the social practices offered by education, primarily time in school and access to workplace-based training. Furthermore, the process of learning a vocation is a complex social venture and the findings argue that there is a risk in attributing students’ struggles solely to issues pertaining to vocational learning as this may overlook underlying structural challenges faced by students.
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| 6. |
- Lind, Lars, et al.
(author)
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Plasma Protein Profile of Carotid Artery Atherosclerosis and Atherosclerotic Outcomes : Meta-Analyses and Mendelian Randomization Analyses
- 2021
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In: Arteriosclerosis, Thrombosis and Vascular Biology. - : Lippincott Williams & Wilkins. - 1079-5642 .- 1524-4636. ; 41:5, s. 1777-1788
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Journal article (peer-reviewed)abstract
- OBJECTIVE: To identify causal pathophysiological mechanisms for atherosclerosis and incident cardiovascular events using protein measurements.APPROACH AND RESULTS: Carotid artery atherosclerosis was assessed by ultrasound, and 86 cardiovascular-related proteins were measured using the Olink CVD-I panel in 7 Swedish prospective studies (11 754 individuals). The proteins were analyzed in relation to intima-media thickness in the common carotid artery (IMT-CCA), plaque occurrence, and incident cardiovascular events (composite end point of myocardial infarction or ischemic stroke) using a discovery/replication approach in different studies. After adjustments for traditional cardiovascular risk factors, 11 proteins remained significantly associated with IMT-CCA in the replication stage, whereas 9 proteins were replicated for plaque occurrence and 17 proteins for incident cardiovascular events. NT-proBNP (N-terminal pro-B-type natriuretic peptide) and MMP (matrix metalloproteinase)-12 were associated with both IMT-CCA and incident events, but the overlap was considerably larger between plaque occurrence and incident events, including MMP-12, TIM-1 (T-cell immunoglobulin and mucin domain 1), GDF (growth/differentiation factor)-15, IL (interleukin)-6, U-PAR (urokinase plasminogen activator surface receptor), LOX-1 (lectin-like oxidized LDL [low-density lipoprotein] receptor 1), and TRAIL-R2 (TNF [tumor necrosis factor]-related apoptosis-inducing ligand receptor 2). Only MMP-12 was associated with IMT-CCA, plaque, and incident events with a positive and concordant direction of effect. However, a 2-sample Mendelian randomization analysis suggested that increased MMP-12 may be protective against ischemic stroke (P=5.5x10(-7)), which is in the opposite direction of the observational analyses.CONCLUSIONS: The present meta-analysis discovered several proteins related to carotid atherosclerosis that partly differed in their association with IMT-CCA, plaque, and incident atherosclerotic disease. Mendelian randomization analysis for the top finding, MMP-12, suggests that the increased levels of MMP-12 could be a consequence of atherosclerotic burden rather than the opposite chain of events.
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| 7. |
- Mustaquim, Moyen, 1976-, et al.
(author)
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A System Development Life Cycle for Persuasive Design for Sustainability
- 2015
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In: Persuasive Technology. - Cham : Springer International Publishing. - 9783319203058 - 9783319203065 ; , s. 217-228
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Book chapter (peer-reviewed)abstract
- The impact of a system development lifecycle (SDLC) often determines the success of a project from analysis to evolution. Although SDLC can be universally used design projects, a focused SDLC for a specific complex design issue could be valuable for understanding diverse user needs. The importance of sustainability elevation using a persuasive system is not new. Previous research presented frameworks and design principles for persuasive system design for sustainability, while an SDLC of sustainable system development also exists. However, at present no SDLC for persuasive design aiming for sustainability is evident, which was proposed in this paper. An existing sustainable SDLC established earlier by the authors was taken as the reference framework. A cognitive model with established persuasive design principles was then analyzed and mapped within the context of the reference framework to come up with the resulting life cycle. Finally, extensive discussions and future work possibilities were given.
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| 8. |
- Mustaquim, Moyen, 1976-, et al.
(author)
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An Inclusive Framework for Developing Video Games for Learning
- 2012
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In: Proceedings of the 6th European Conference on Games Based Learning (ECGBL 2012). - Reading, UK : Academic Publishing International Limited. - 9781908272690 - 9781908272706 ; , s. 348-355
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Conference paper (peer-reviewed)abstract
- Games are a type of vehicle with which society is changing at present-day. But how the games themselves will be changed by society, is an interesting question to ask for enhancing and initiating better design of games. As participation in gaming upsurges around the world and across a larger part of the population, it is obvious that games are not only an entertainment medium for children or young people anymore. Games can be used to accomplish different purposes for different groups of people in the society. Design and use of games for learning and/or educational purpose has been an important research field for several years. However, this research field is yet to deliver expected achievement and result, despite many efforts and resources put into it. Factors that are important to consider for designing a game that will motivate users to active involvement in playing a game for active learning, are crucial for the successfulness of the design of such a game. The design goal for educational games would be to produce games that motivate users and thereby achieve effective learning. This paper proposes a framework in which the parameters of what users want from a game are considered for the development of games for learning. The framework is based on the previous research and theory of Richard Rouse (2001), who defined different dimensions of game design in terms of learning. The proposed framework is important in order to investigate and assess the success of existing games used for learning. Also the framework can provide better understanding as a requirement engineering tool for game designers, who are to design games for learning and educational purposes in the future. The proposed framework was verified using a small quantitative analysis. It was found that, while some parameters of the framework indeed have significance for designing games for learning, there are additional factors that need to be discovered by further analysis and research to enhance the usability of the framework.
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| 9. |
- Mustaquim, Moyen, 1976-, et al.
(author)
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An Iterative Information System Design Process for Sustainability
- 2015
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In: SIGCHI HCI & Sustainability Workshop - CHI '15.
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Conference paper (peer-reviewed)abstract
- Bridging business and computer science into an improvedalignment using the theoretical foundations of informationand computation is one of the aims of information science(IS). Improved design knowledge from other interdisciplinaryresearch fields, like human-computer interaction (HCI),could advance different design thinking and processes forIS. While structuring an IS design process for a sustainableresult is challenging, an HCI-focused viewpoint on IS designcould be beneficial in this matter. In this paper, an iterativedesign process for sustainable information system designconceptualized from HCI is proposed. The resulting designprocess highlights the different roles of HCI towards buildingknowledge in IS by influencing different design choices onuser behavior and contributing towards generating reusabledesigns in different phases of the design process.
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| 10. |
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