| 1. |
- Olofsson, Thomas, et al.
(author)
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The InPro Lifecycle Design Framework for Buildings
- 2010
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Reports (other academic/artistic)abstract
- On average, by the time 1% of project costs are spent, roughly 70% of the lifecycle costof the building has been committed indicating that benefits of integration are largest inthe early phases of a project. The building shape, selected materials, structural system,internal room distribution, and building services systems are some of the most importantfactors that influence the costs of operation and upgrading throughout the lifecycle.The main goal of the InPro project is to shift focus from the detail design to the earlyphase where the majority of the decisions are taken that influence the total performanceof the building. Therefore, the work in task 2.4 has been aimed at developing an integratedformalized iterative lifecycle design where project goals can be matched againstkey performance indictors (KPI:s) in the design process. The methodology used is acombination of literature reviews; interviews with clients, contractors and energy consultants;the participants own experience; workshops and project meetings within theInPro consortium and the development of demonstration scenarios.The building life cycle treated in this report has been limited to the early design includingmainly the operational aspects on costs and environment. The effect of repair, replacementand demolishing has not been treated.The result of task 2.4 is the InPro life cycle design framework consisting of: The InPro Smart decision making where project goals and functional needs aremapped to building performance requirements. The InPro stage/gate design where the information maturity is adapted to theproject specific decision making process. The InPro lifecycle maturity levels to guide the project management using theInPro design framework. Change management procedures are applied on approvedmaturity levels in the Open Information Platform (OIP). The InPro workflow process between two decision quality gates containing performancerequirement processing, developing a design strategy, concurrentdesign and analysis process and information quality assurance.The InPro early design framework is demonstrated in three design scenarios with focuson energy performance, environmental assessment and operation.The following recommendations are made regarding the investigated life cycle aspect: Analysis of energy and indoor climate related KPI:s and comparison with performancerequirements can be made when the OIP maturity is such that indoorclimate simulation is possible to perform on room level. Energy performance analyses should be conducted before the structural andHVAC system design is finalized since the result will guide the structural andbuilding service designer in the selection of structural system, the buildingshell and the HVAC system. Training and commitment of the end users are also needed to motivate changetowards a more sustainability and energy saving behaviour of the users byproviding feedback and user-friendly control of building installations. The procedure for environmental evaluation is based on the LCA method andcomplemented with a check of the occurrence of hazardous chemical substances. Requirements on environmental performance should be clearly defined foreach project with respect to the client’s values.Report – The InPro Lifecycle Design Framework for Buildings ■ January 2010 6/164 Objects in the models need to be complemented with information that can belinked to cost information in building parts libraries to speed-up the cost estimationsprocess. However, the model based cost estimation covers only a partof the total Life Cycle Cost and need to be complemented with other investmentsand operational aspects not directly linked to the models.The following important actors/roles/competences have been identified in the earlystages of the InPro Life Cycle design framework: It is recommended that the client is actively involved not only via briefing sessionand decision-making at decision making quality gates, but also in the designthrough the open information platform giving access to monitor and interactdirectly with the design team throughout the design process. Energy and environmental analyst should actively take part in the design process,given the opportunity to affect the building design in the early phases of aproject. This will guide architects, structural engineers and HVAC designers ina more sustainable direction. The Facility and Maintenance specialist plays an important role bridging thegap between design and construction and operations. Their knowledge and experienceprovide valuable contribution in the early design process of a buildingslife cycle performance. A new role as project information manager is proposed handling model aggregationand the quality assurance of the information stored in the OIP. In a shared information environment like InPro the responsible and risk involvedwith quantity surveying must be resolved. It is suggested that onerole/actor is dedicated to the responsibility of quantity surveying and to updatequantities in the OIP when new design models are created or changed. Towork well, the risk as well as the quantity information needs to be sharedamong project participants.Regarding the implementation of the InPro Life Cycle framework the following steps arerecommended: The visualisation step: The participating organisation need to be trained inmodel based working routines where different design disciplines 3D models areaggregated in digital mock-ups. Gains in clarification of project objectives forstakeholders and resolving of coordination issues between different design disciplinescan justify the relative inexpensive investments made on project level. The integration step rely on computer based methods to exchange data amongdifferent modelling and analysis application either using standard formats suchas IFC (International Foundation Classes) or propriety formats. Lessons fromthe manufacturing domain has shown that an integrated concurrent engineeringdesign process need to be stage/gated and supported by central repositoryof shared information under change management control. The integration stepwill be more expensive to implement and requires long term relations betweenparticipating organisations (over several projects).
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| 2. |
- Sävenstrand, Helena, et al.
(author)
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Induction of early light-inducible protein gene expression in Pisum sativum after exposure to low levels of UV-B irradiation and other environmental stresses
- 2004
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In: Plant Cell Reports. - : Springer Science and Business Media LLC. - 0721-7714 .- 1432-203X. ; 22:7, s. 532-536
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Journal article (peer-reviewed)abstract
- Plants are constantly subjected to environmental changes and have developed various defence mechanisms to facilitate their continued existence. Pisum sativum plants were exposed to low levels of UV-B radiation and ELIP (early light-inducible proteins) mRNA, with a probable protective function, was rapidly and strongly induced during this type of stress. To our knowledge, this is the only photosynthetic gene that is up-regulated following exposure to UV-B, and this result has to be compared with studies predominantly reporting down-regulation by UV-B of genes encoding proteins localised in the plastid. The expression pattern of ELIP mRNA in pea was also investigated during salt, wounding and ozone stress. The transcript levels of ELIP were induced after the salt and wounding treatments but not during ozone fumigation.
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| 3. |
- Blomqvist, Ulf, et al.
(author)
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DRM – Intrusion or Solution?
- 2005
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In: Innovation and the knowledge economy. - Amsterdam : IOS Press. ; , s. 925-933
-
Conference paper (peer-reviewed)abstract
- DRM could be the solution to the content industry’s P2P dilemma, but content owners’ desire to monitor and control the consumer’s use of content can be perceived as an intrusion of privacy. High control makes consumers less active and low control invites them to experiment and to gain experience. Digital music files can easily be spread. Therefore legal services prefer keeping control over the music. This study reveals that consumers are one step ahead of the content industry, finding ways to circumvent protection and laws, and that the music industry is awaiting the "right" DRM business model. Online Music sellers basically rely on B2C DRM business models, but attempts have been made with C2C models, using consumers as distributors, and ISP models, where ISPs pay to collecting societies for all file trans-fers of copyrighted material in their networks. Future models include licensing mod-els, micro models, and no copyright at all.
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| 4. |
- Boström, Pontus, 1982, et al.
(author)
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Hypoxia converts human macrophages into triglyceride-loaded foam cells.
- 2006
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In: Arteriosclerosis, thrombosis, and vascular biology. - 1524-4636. ; 26:8, s. 1871-6
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Journal article (peer-reviewed)abstract
- OBJECTIVE: Atherosclerotic lesions have regions that are hypoxic. Because the lesion contains macrophages that are loaded with lipid, we investigated whether hypoxia can influence the accumulation of lipids in these cells. METHODS AND RESULTS: Exposure of human macrophages to hypoxia for 24 hours resulted in an increased formation of cytosolic lipid droplets and an increased accumulation of triglycerides. Exposure of the macrophages to oxidized low-density lipoprotein (oxLDL) increased the accumulation of cytosolic lipid droplets because of an increase in cellular cholesterol esters. The accumulation of lipid droplets in oxLDL-treated cells was further increased after hypoxia, caused by an increased level of triglycerides. Expression analyses combined with immunoblot or RT-PCR demonstrated that hypoxia increased the expression of several genes that could promote the accumulation of lipid droplets. Hypoxia increased the mRNA and protein levels of adipocyte differentiation-related protein (ADRP). It is well known that an increased expression of ADRP increases the formation of lipid droplets. Hypoxia decreased the expression of enzymes involved in beta-oxidation (acyl-coenzyme A synthetase and acyl-coenzyme A dehydrogenase) and increased the expression of stearoyl-coenzyme A desaturase, an important enzyme in the fatty acid biosynthesis. Moreover, exposure to hypoxia decreased the rate of beta-oxidation, whereas the accumulation of triglycerides increased. CONCLUSIONS: The results demonstrate that exposure of human macrophages to hypoxia causes an accumulation of triglyceride-containing cytosolic lipid droplets. This indicates that the hypoxia present in atherosclerotic lesions can contribute to the formation of the lipid-loaded macrophages that characterize the lesion and to the accumulation of triglycerides in such lesions.
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| 5. |
- Byström, Roberth, 1971-, et al.
(author)
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Disordered proteins : Biological membranes as two-dimensional aggregation matrices
- 2008
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In: Cell Biochemistry and Biophysics. - : Springer Science and Business Media LLC. - 1085-9195 .- 1559-0283. ; 52:3, s. 175-189
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Research review (peer-reviewed)abstract
- Aberrant folded proteins and peptides are hallmarks of amyloidogenic diseases. However, the molecular processes that cause these proteins to adopt non-native structures in vivo and become cytotoxic are still largely unknown, despite intense efforts to establish a general molecular description of their behavior. Clearly, the fate of these proteins is ultimately linked to their immediate biochemical environment in vivo. In this review, we focus on the role of biological membranes, reactive interfaces that not only affect the conformational stability of amyloidogenic proteins, but also their aggregation rates and, probably, their toxicity. We first provide an overview of recent work, starting with findings regarding the amphiphatic amyloid-β protein (Aβ), which give evidence that membranes can directly promote aggregation, and that the effectiveness in this process can be related to the presence of specific neuronal ganglioside lipids. In addition, we discuss the implications of recent research (medin as an detailed example) regarding putative roles of membranes in the misfolding behavior of soluble, non-amphiphatic proteins, which are attracting increasing interest. The potential role of membranes in exerting the toxic action of misfolded proteins will also be highlighted in a molecular context. In this review, we discuss novel NMR-based approaches for exploring membrane–protein interactions, and findings obtained using them, which we use to develop a molecular concept to describe membrane-mediated protein misfolding as a quasi-two-dimensional process rather than a three-dimensional event in a biochemical environment. The aim of the review is to provide researchers with a general understanding of the involvement of membranes in folding/misfolding processes in vivo, which might be quite universal and important for future research concerning amyloidogenic and misfolding proteins, and possible ways to prevent their toxic actions.
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| 6. |
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| 7. |
- Castoldi, Laura, et al.
(author)
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Electrophilic Vinylation of Thiols under Mild and Transition Metal-Free Conditions
- 2020
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In: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 59:36, s. 15512-15516
-
Journal article (peer-reviewed)abstract
- The iodine(III) reagents vinylbenziodoxolones (VBX) were employed to vinylate a series of aliphatic and aromatic thiols, providing E-alkenyl sulfides with complete chemo- and regioselectivity, as well as excellent stereoselectivity. The methodology displays high functional group tolerance and proceeds under mild and transition metal-free conditions without the need for excess substrate or reagents. Mercaptothiazoles could be vinylated under modified conditions, resulting in opposite stereoselectivity compared to previous reactions with vinyliodonium salts. Novel VBX reagents with substituted benziodoxolone cores were prepared, and improved reactivity was discovered with a dimethyl-substituted core.
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| 8. |
- Cinato, Mathieu, et al.
(author)
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Cardiac Plin5 interacts with SERCA2 and promotes calcium handling and cardiomyocyte contractility
- 2023
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In: Life Science Alliance. - : Life Science Alliance, LLC. - 2575-1077. ; 6:4
-
Journal article (peer-reviewed)abstract
- The adult heart develops hypertrophy to reduce ventricular wall stress and maintain cardiac function in response to an increased workload. Although pathological hypertrophy generally prog-resses to heart failure, physiological hypertrophy may be car-dioprotective. Cardiac-specific overexpression of the lipid-droplet protein perilipin 5 (Plin5) promotes cardiac hypertrophy, but it is unclear whether this response is beneficial. We analyzed RNA -sequencing data from human left ventricle and showed that car-diac PLIN5 expression correlates with up-regulation of cardiac contraction-related processes. To investigate how elevated cardiac Plin5 levels affect cardiac contractility, we generated mice with cardiac-specific overexpression of Plin5 (MHC-Plin5 mice). These mice displayed increased left ventricular mass and cardiomyocyte size but preserved heart function. Quantitative proteomics identified sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 2 (SERCA2) as a Plin5-interacting protein. In situ proximity ligation assay further confirmed the Plin5/SERCA2 interaction. Live imaging showed in-creases in intracellular Ca2+ release during contraction, Ca2+ removal during relaxation, and SERCA2 function in MHC-Plin5 versus WT cardiomyocytes. These results identify a role of Plin5 in improving cardiac contractility through enhanced Ca2+ signaling.
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| 9. |
- Dahlqvist, Jörgen, et al.
(author)
-
Fält
- 2015
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Artistic work (other academic/artistic)
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| 10. |
- Dahlqvist, Jörgen
(author)
-
Stränder
- 2014
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Artistic work (other academic/artistic)
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