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- Mollenhauer, Jan, et al.
(author)
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Carcinogen inducibility in vivo and down-regulation of DMBT1 during breast carcinogenesis.
- 2004
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In: Genes, chromosomes & cancer. - : Wiley. - 1045-2257. ; 39:3, s. 185-94
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Journal article (peer-reviewed)abstract
- Deleted in malignant brain tumors 1 (DMBT1) has been proposed as a candidate tumor suppressor for brain and epithelial cancer. Initial studies suggested loss of expression rather than mutation as the predominant mode of DMBT1 inactivation. However, in situ studies in lung cancer demonstrated highly sophisticated changes of DMBT1 expression and localization, pointing to a chronological order of events. Here we report on the investigation of DMBT1 in breast cancer in order to test whether these principles might also be attributable to other tumor types. Comprehensive mutational analyses did not uncover unambiguous inactivating DMBT1 mutations in breast cancer. Expression analyses in the human and mouse mammary glands pointed to the necessity of DMBT1 induction. While age-dependent and hormonal effects could be ruled out, 9 of 10 mice showed induction of Dmbt1 expression after administration of the carcinogen 7,12-dimethybenz(alpha)anthracene prior to the onset of tumorigenesis or other histopathological changes. DMBT1 displayed significant up-regulation in human tumor-flanking tissues compared to in normal breast tissues (P < 0.05). However, the breast tumor cells displayed a switch from lumenal secretion to secretion to the extracellular matrix and a significant down-regulation compared to that in matched normal flanking tissues (P < 0.01). We concluded that loss of expression also is the predominant mode of DMBT1 inactivation in breast cancer. The dynamic behavior of DMBT1 in lung carcinoma is fully reflected in breast cancer, which suggests that this behavior might be common to tumor types arising from monolayered epithelia.
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| 2. |
- Simonsson, Bengt, et al.
(author)
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Intensive treatment and stem cell transplantation in chronic myelogenous leukemia : long-term follow-up
- 2005
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In: Acta Haematologica. - : S. Karger AG. - 0001-5792 .- 1421-9662. ; 113:3, s. 155-162
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Journal article (peer-reviewed)abstract
- In the present study we combined interferon (IFN) and hydroxyurea (HU) treatment, intensive chemotherapy and autologous stem cell transplantation (SCT) in newly diagnosed chronic myelogenous leukemia patients aged below 56 years, not eligible for allogeneic SCT. Patients who had an HLA-identical sibling donor and no contraindication went for an allogeneic SCT (related donor, RD). After diagnosis, patients not allotransplanted received HU and IFN to keep WBC and platelet counts low. After 6 months patients with Ph-positive cells still present in the bone marrow received 1–3 courses of intensive chemotherapy. Those who became Ph-negative after IFN + HU or after 1–3 chemotherapy courses underwent autologous SCT. Some patients with poor cytogenetic response were allotransplanted with an unrelated donor (URD). IFN + HU reduced the percentage of Ph-positive metaphases in 56% of patients, and 1 patient became Ph-negative. After one or two intensive cytotherapies 86 and 88% had a Ph reduction, and 34 and 40% became Ph-negative, respectively. In patients receiving a third intensive chemotherapy 92% achieved a Ph reduction and 8% became Ph-negative. The median survival after auto-SCT (n = 46) was 7.5 years. The chance of remaining Ph-negative for up to 10 years after autologous SCT was around 20%. The overall survival for allo-SCT RD (n = 91) and URD (n = 28) was almost the same, i.e. ≈60% at 10 years. The median survival for all 251 patients registered was 8 years (historical controls 3.5 years). The role of the treatment schedule presented in the imatinib era is discussed.
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| 3. |
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| 4. |
- Geijerstam, Jan af
(author)
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Statarnas sista flyttlass
- 1995
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In: Populär historia. - Lund : Populär historia. - 1102-0822. ; :5, s. 54-57
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Journal article (pop. science, debate, etc.)
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| 5. |
- Hyltander, Anders, 1954, et al.
(author)
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Supportive nutrition on recovery of metabolism, nutritional state, health-related quality of life, and exercise capacity after major surgery: a randomized study
- 2005
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In: Clinical gastroenterology and hepatology. - 1542-3565. ; 3:5, s. 466-74
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Journal article (peer-reviewed)abstract
- BACKGROUND & AIMS: The aim of this study was to investigate whether specialized supportive enteral and parenteral feeding have superior effects compared to oral nutrition on recovery during long-term postoperative treatment of cancer patients with preoperative weight loss and reduced maximum exercise capacity. METHODS: One hundred twenty-six patients referred for resection of the esophagus (n = 48), stomach (n = 28), or pancreas (n = 50) were considered to be included before operation. Included patients (n = 80) received supportive enteral or parenteral nutrition postoperatively at home corresponding to 1000 kcal/d until the patients did not wish to continue with artificial nutrition for any reason. Patients randomized to oral nutrition only served as control subjects. Caloric intake, body composition (dual-energy x-ray absorptiometry), and respiratory gas exchanges at rest and during exercise were measured including health-related quality of life. RESULTS: Survival and hospital stay did not differ among the groups, whereas overall complications were higher on artificial nutrition (P < .05). Changes in resting energy expenditure and biochemical tests did not differ during follow-up among the groups. Body weight and whole body fat declined similarly over time in all groups (P < .005), whereas lean body mass was unchanged during follow-up compared to preoperative values. Maximum exercise capacity and maximum oxygen consumption were normalized within 6 months postoperatively in all groups. There was no difference in recovery of food intake among the groups. Parenteral feeding was associated with the highest rate of nutrition-related complications, whereas enteral feeding reduced quality of life most extensively. CONCLUSION: After major surgery, specialized supportive enteral and parenteral nutrition are not superior to oral nutrition only when guided by a dietitian.
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| 6. |
- Källtorp, Mia, et al.
(author)
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Analysis of rat plasma proteins desorbed from gold and methyl- and hydroxyl-terminated alkane thiols on gold surfaces.
- 2000
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In: Journal of materials science. Materials in medicine. - 0957-4530 .- 1573-4838. ; 11:3, s. 191-9
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Journal article (peer-reviewed)abstract
- It is believed that adsorbed blood or plasma components, such as water, peptides, carbohydrates and proteins, determine key events in the concomitant inflammatory tissue response close to implants. The aim of the present study was to develop a procedure for the collection and analysis of minor amounts of proteins bound to solid metal implant surfaces. The combination of a sodium dodecyl sulfate washing method coupled with a polyacylamide gel electrophoretic protein separation technique (SDS-PAGE), Western blot and image analysis enabled the desorption, identification and semiquantification of specific proteins. The analyzed proteins were albumin, immunoglobulin G, fibrinogen and fibronectin. Concentration procedures of proteins were not required with this method despite the small area of the test surfaces. The plasma proteins were adsorbed to pure gold and hydroxylated and methylated gold surfaces, which elicit different tissue responses in vivo and plasma protein adsorption patterns in vitro. The image analysis revealed that the pure gold surfaces adsorbed the largest amount of total and specific proteins. This is in accordance with previous ellipsometry/antibody experiments in vitro. Further, the principles described for the protein analysis can be applied on implant surfaces ex vivo.
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| 7. |
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| 8. |
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| 9. |
- Nguyen, P T M, et al.
(author)
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Antimicrobial actions of benzimidazoles against oral streptococci.
- 2005
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In: Oral microbiology and immunology. - 0902-0055. ; 20:2, s. 93-100
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Journal article (peer-reviewed)abstract
- BACKGROUND/AIM: Benzimidazoles, such as lansoprazole and omeprazole, are used extensively as proton-pump inhibitors to control stomach acid secretion and also have antimicrobial actions against Helicobacter pylori. Our objective was to determine whether they are potentially useful antimicrobials against oral bacteria. METHODS: Streptococcus mutans was our main test organism. It was grown in suspension cultures and biofilms. Standard physiologic assays were used to assess inhibitory actions of benzimidazoles. RESULTS: Benzimidazoles inhibited acid production by S. mutans in suspensions or biofilms. In pH-drop experiments, lansoprazole at a level of only 0.025 mm irreversibly inhibited acid production from glycolysis. Cell uptake of lansoprazole was found to be very pH sensitive and occurred mainly at pH values below about 5, indicating that the protonated form was taken up. Lansoprazole inhibition of glycolysis could be blocked by 2-mercaptoethanol, which suggests that disulfide bonds form between benzimidazoles and protein targets. Identified targets for benzimidazole inhibition included the phosphoenolpyruvate : sugar phosphotransferase system, the glycolytic enzymes aldolase, glyceraldehyde-3-phosphate dehydrogenase, and lactic dehydrogenase, and enzymes such as urease and arginine deiminase. Lansoprazole increased proton permeabilities of S. mutans cells but did not inhibit F-ATPases. Although cells in biofilms were somewhat less sensitive to the agents than those in suspensions, biofilm glycolysis was still markedly inhibited by 0.1 mm lansoprazole. Benzimidazoles are bactericidal, and the oral anaerobes Fusobacterium nucleatum and Prevotella intermedia were more sensitive to killing than was S. mutans. CONCLUSION: Benzimidazoles appear to be useful inhibitors of oral bacteria in acid environments such as progressing caries lesions.
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| 10. |
- Olsson, Anders, 1973-, et al.
(author)
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Nytt paradigm för hållfasthetssortering av konstruktionsvirke : Projektrapport två
- 2014
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Reports (other academic/artistic)abstract
- Undersökningen som redovisas i denna rapport har genomförts under hösten 2013 och våren 2014 inom projektet "Nytt paradigm för hållfasthetssortering av konstruktionsvirke". Projektet, som pågår under perioden december 2011 till juni 2014, går ut på att för industriellt bruk utveckla en nyligen föreslagen metod för hållfasthetssortering av konstruktionsvirke, vilken baseras på laserskanning i kombination med dynamisk excitering och densitetsbestämning av virke. Projektet finansieras till 50 % av VINNOVA och till 50 % av de företag som medverkar i projektet, nämligen Innovativ Vision AB (IV), Vida Vislanda AB, Södra Timber AB, Derome Timber AB och Dynalyse AB. Linnéuniversitetet (Lnu) och SP Sveriges Tekniska Forskningsinstitut AB (SP) samverkar med nämnda företag i utförandet av forskningen.Ett syfte med undersökningen är att på ett stort material bestående av många olika virkesdimensioner verifiera de starka statistiska samband som sedan tidigare påvisats för ett begränsat antal plankor och dimensioner, mellan å ena sidan indikerande egenskaper som baseras på mätningar och beräkningar och å andra sidan böjstyrka, elasticitetsmodul och densitet. Ett annat syfte med undersökningen är att visa att nödvändig data kan samlas in av samma utrustning och under samma förhållanden som kommer att gälla vid framtida hållfasthetssortering baserad på den aktuella metoden. Slutligen är ett syfte att samla in och dokumentera data som kan användas för forskning och utveckling både inom och efter det att det aktuella projektet har avslutats.
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