| 1. |
- Burri, Stina, et al.
(author)
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Processed meat products with added plant antioxidants affect the microbiota and immune response in C57BL/6JRj mice with cyclically induced chronic inflammation
- 2021
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In: Biomedicine and Pharmacotherapy. - : Elsevier BV. - 1950-6007 .- 0753-3322. ; 135
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Journal article (peer-reviewed)abstract
- Epidemiological studies have found that there is a correlation between red and processed meat consumption and an increased risk of colorectal cancer. There are numerous existing hypotheses on what underlying mechanisms are causative to this correlation, but the results remain unclear. A common hypothesis is that lipid oxidation, which occurs in endogenous lipids and phospholipids in consumed food, are catalyzed by the heme iron in meat. In this study, five pre-selected plant antioxidant preparations (sea buckthorn leaves and sprouts, summer savory leaves, olive polyphenols, onion skin and lyophilized black currant leaves) were added to a meatball type prone to oxidize (pork meat, 20 % fat, 2% salt, deep-fried and after 2 weeks of storage). Pro-inflammatory markers, neutrophil infiltration and microbiota composition were studied after four months in a chronic inflammation model in C57BL6/J female mice. We found that the bacterial diversity index was affected, as well as initial immunological reactions.
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| 2. |
- Canaviri-Paz, Pamela, et al.
(author)
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Autochthonous microorganisms of white quinoa grains with special attention to novel functional properties of lactobacilli strains
- 2021
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In: Journal of Functional Foods. - : Elsevier BV. - 1756-4646. ; 84
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Journal article (peer-reviewed)abstract
- Quinoa sourdough can safely be produced if the hygienic threshold of miss-fermentation during the start-up period is overcome. The aims of the present study are to map the microbiota of spontaneously formed quinoa sourdough without backslopping and select Lactiplantibacillus-strains. Viable count from grains and sourdough were performed and picked isolates were identified by gene sequencing. Lactiplantibacillus interspecies differentiation was evaluated by specific primers targeting the recA region, fermentation capacity and enzymatic abilities applying colorimetric assays, respectively. High initial levels of Enterobacteriaceae and other opportunistic pathogens decreased gradually, and the pH dropped below 4. The results indicate that spontaneous fermentation involves a variety of quinoa autochthonous microorganisms, with low presence of lactobacilli due to the delay lactic acid fermentation. Lactobacilli strains able to ferment xylose and glycerol and metabolize phenolic compounds were identified holding great prospects as suitable starter cultures for production of fermented quinoa products.
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| 3. |
- Canaviri Paz, Pamela Rosario, et al.
(author)
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Effects on Microbiota Composition after Consumption of Quinoa Beverage Fermented by a Novel Xylose-Metabolizing L. plantarum strain
- 2021
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In: Nutrients. - : MDPI AG. - 2072-6643. ; 13:10
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Journal article (peer-reviewed)abstract
- Demands for novel lactic acid bacteria with potential to be used as probiotics along with healthy fermented plant-based products increase worldwide. In this study, a novel Lactiplantibacillus plantarum P31891 strain with enzymatic capacity to degrade tannins and ferment xylose was used as starter culture for fermentation of a quinoa-based beverage. The probiotic potential of the selected strain was evaluated in healthy volunteers. Twenty participants consumed the beverage for 14 days; microbiota changes in saliva and faecal samples were analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP), Next Generation Sequencing (NGS) and qPCR; and gastrointestinal well-being and digestive symptoms were recorded. The results indicated that the consumption of the beverage with Lactiplantibacillus plantarum P31891 in a probiotic dose (1012 CFU/mL) increased the number of Lactobacillus in the feces but not in saliva. Overall, the bacterial community did not seem to be influenced by the bacterium or by the beverage, as expressed by the diversity indexes, butspecific genera were affected, as reflected in changes in amplicon sequence variants. Consequently, Lactiplantibacillus plantarum P31891 showed potential to be categorized as a probiotic strain in the fermented quinoa-based beverage.
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| 4. |
- Hurych, Jakub, et al.
(author)
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Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity : a randomized, double-blind placebo-controlled clinical trial
- 2023
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In: Parasites and Vectors. - 1756-3305. ; 16:1
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Journal article (peer-reviewed)abstract
- Background: Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 positively affect the fecal bacteriome in children with celiac disease autoimmunity after 6 months of supplementation. The aim of the present investigation was to study the effects of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 on the single-cell parasitome, with a primary focus on Blastocystis. Methods: Stool samples were collected from 78 Swedish children with celiac disease autoimmunity participating in a randomized, double-blind, placebo-controlled clinical trial to either receive a mixture of supplementation with L. plantarum HEAL9 and L. paracasei 8700:2 (n = 38) or placebo (n = 40). A total of 227 stool samples collected at baseline and after 3 and 6 months of intervention, respectively, were retrospectively analyzed for Blastocystis by quantitative real-time PCR and subtyped by massively parallel amplicon sequencing. Other single-cell parasites were detected by untargeted 18S rDNA amplicon sequencing and verified by real-time PCR. The relation between the parasites and the bacteriome community was characterized by using 16S rDNA profiling of the V3-V4 region. Results: Three different single-cell protists were identified, of which the highest prevalence was found for Dientamoeba fragilis (23.1%, 18/78 children), followed by Blastocystis (15.4%, 12/78) and Entamoeba spp. (2.6%, 2/78). The quantity of the protists was stable over time and not affected by probiotic intervention (P = 0.14 for Blastocystis, P = 0.10 for D. fragilis). The positivity of the protists was associated with increased bacteriome diversity (measured by multiple indices, P < 0.03). Bacterial composition was influenced by the presence of the protists: positivity of Blastocystis was inversely associated with Akkermansia (at the levels of the genus as well as its family, order, class and phylum); P < 0.002), Faecalibacterium (P = 0.003) and Romboutsia (P = 0.029); positivity of D. fragilis was inversely associated with families Enterobacteriaceae (P = 0.016) and Coriobacteriaceae (P = 0.022) and genera Flavonifractor (P < 0.001), Faecalibacterium (P = 0.009), Lachnoclostridium (P = 0.029), Ruminococcus (P < 0.001) and Granulicatella (P = 0.018). Conclusions: The prevalence of single-cell protists is low in children with celiac disease autoimmunity. The colonization was stable regardless of the probiotic intervention and associated with increased diversity of the fecal bacteriome but inversely associated with some beneficial bacteria. Graphical Abstract: [Figure not available: see fulltext.]
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| 5. |
- Håkansson, Åsa, et al.
(author)
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Effects of lactobacillus plantarum and lactobacillus paracasei on the peripheral immune response in children with celiac disease autoimmunity : A randomized, double-blind, placebo-controlled clinical trial
- 2019
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In: Nutrients. - : MDPI AG. - 2072-6643. ; 11:8
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Journal article (peer-reviewed)abstract
- Two Lactobacillus strains have proven anti-inflammatory properties by reducing pro-inflammatory responses to antigens. This randomized double-blind placebo-controlled trial tested the hypothesis that L. plantarum HEAL9 and L. paracasei 8700:2 suppress ongoing celiac disease autoimmunity in genetically at risk children on a gluten-containing diet in a longitudinally screening study for celiac disease. Seventy-eight children with celiac disease autoimmunity participated of whom 40 received 1010 CFU/day of L. plantarum HEAL9 and L. paracasei 8700:2 (probiotic group) and 38 children maltodextrin (placebo group) for six months. Blood samples were drawn at zero, three and six months and phenotyping of peripheral blood lymphocytes and IgA and IgG autoantibodies against tissue transglutaminase (tTG) were measured. In the placebo group, naïve CD45RA+ Th cells decreased (p = 0.002) whereas effector and memory CD45RO+ Th cells increased (p = 0.003). In contrast, populations of cells expressing CD4+CD25high CD45RO+CCR4+ increased in the placebo group (p = 0.001). Changes between the groups were observed for NK cells (p = 0.038) and NKT cells (p = 0.008). Median levels of IgA-tTG decreased more significantly over time in the probiotic (p = 0.013) than in the placebo (p = 0.043) group whereas the opposite was true for IgG-tTG (p = 0.062 respective p = 0.008). In conclusion, daily oral administration of L. plantarum HEAL9 and L. paracasei 8700:2 modulate the peripheral immune response in children with celiac disease autoimmunity.
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| 6. |
- Marefati, Ali, et al.
(author)
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Encapsulation of Lactobacillus reuteri in W1/O/W2 double emulsions : Formulation, storage and in vitro gastro-intestinal digestion stability
- 2021
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In: LWT. - : Elsevier BV. - 0023-6438. ; 146
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Journal article (peer-reviewed)abstract
- Encapsulation of Lactobacillus reuteri in the internal aqueous phase of W1/O/W2 emulsions was investigated. Microstructure of the emulsions was evaluated using a particle size analyzer and light microscopy. The encapsulation properties were evaluated in terms of encapsulation efficiency (EE) for freshly prepared samples and encapsulation stability (ES) during storage and in vitro digestion and the results were compared to control. Particle size analysis showed that encapsulation of the bacteria increased the droplet sizes of W1/O emulsions significantly (P < 0.05) from 3.5 ± 0.2 to 4.8 ± 0.4 μm which was verified by microscopy. Neither encapsulation nor storage changed (P > 0.05) the droplet sizes for double emulsions (14.9 ± 0.3 μm) compared to control (15.5 ± 0.4 μm) which remained stable through storage period. The EE was 7.23 ± 0.07 Log CFU/mL. During cold storage, the ES decreased with a higher pace for control (from 6.18 ± 0.03 to <1 Log CFU/mL) compared to formulations with encapsulated bacteria (from 7.23 ± 0.07 to 2.82 ± 0.10 Log CFU/mL). Finally, during in vitro digestion, the ES decreased with a higher rate for control (from 6.25 ± 0.36 to 2.69 ± 0.06 Log CFU/mL) compared to encapsulated samples (from 6.69 ± 0.07 to 4.64 ± 0.06 Log CFU/mL). The results showed that encapsulation of Lactobacillus reuteri using double emulsions can protect the probiotics during storage and in vitro gastro-intestinal digestion.
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| 7. |
- Naurin, Elin, 1975, et al.
(author)
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Citizens’ Perceptions of Party Pledges
- 2012
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In: Paper presented at the Annual Meeting of the Swedish Political Science Association in Växjö 26-28 September 2012..
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Conference paper (other academic/artistic)
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| 8. |
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| 9. |
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| 10. |
- Naurin, Elin, 1975, et al.
(author)
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When and Why Are Voters Correct in Their Evaluations of Specific Government Performance?
- 2017
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In: Political Studies. - : SAGE Publications. - 0032-3217 .- 1467-9248. ; 65:4, s. 860-876
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Journal article (peer-reviewed)abstract
- Democratic theories expect citizens to be able to accurately evaluate fulfilment of parties’ election pledges. We use specifically designed survey items from the Swedish National Election Study to compare citizens’ perceptions of the fulfilment of specific party pledges with actual fulfilment and assess circumstances that lead to correct evaluations. We find that political knowledge triumphs partisan attachments to incumbent parties when it comes to explaining why voters are correct. The results are interesting in light of common knowledge about the importance of partisan attachment in evaluations of general government performance: We argue that when specific election pledges are being evaluated, personal heuristics, such as attachments to incumbent parties, play a lesser role for judgements. Instead, the specificity embedded in the evaluation encourages citizens to engage in a more knowledge-based evaluation of whether pledges are fulfilled or not.
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