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- Pastula, Agnieszka, et al.
(författare)
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Cellular Interactions in the Intestinal Stem Cell Niche
- 2019
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Ingår i: Archivum Immunologiae et Therapiae Experimentalis. - : Springer. - 0004-069X .- 1661-4917. ; 67:1, s. 19-26
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Forskningsöversikt (refereegranskat)abstract
- Epithelial cells are one of the most actively cycling cells in a mammalian organism and therefore are prone to malignant transformation. Already during organogenesis, the connective tissue (mesenchyme) provides instructive signals for the epithelium. In an adult organism, the mesenchyme is believed to provide crucial regulatory signals for the maintenance and regeneration of epithelial cells. Here, we discuss the role of intestinal myofibroblasts, α-smooth muscle actin-positive stromal (mesenchymal) cells, as an important regulatory part of the intestinal stem cell niche. Better understanding of the cross-talk between myofibroblasts and the epithelium in the intestine has implications for advances in regenerative medicine, and improved therapeutic strategies for inflammatory bowel disease, intestinal fibrosis and colorectal cancer.
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| 2. |
- Pastula, Agnieszka
(författare)
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Immunoscore, circulating tumor cells and human-derived organoids as potential predictive tools in personalized cancer medicine
- 2017
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Ingår i: Medical ethics, prediction, and prognosis. - United Kingdom : Routledge. - 9781138632691 - 9781315208084 ; , s. 175-185
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- In order to validate the immunoscore, an international consortium with twenty-three centers involved was initiated in 2012. It is planned to test immunoscore on tumor samples from several thousand colorectal cancer patients. Observations from tumor immunology have contributed to the findings that immune cell type, density, and location within the tumor could be a prognostic factor, and have thus led to the development of the immunoscore. The tumor cells that are present in the bloodstream are called circulating tumor cells, and are believed to be the direct precursors of the metastases. The tumor staging system staging system is based on the analysis of tumor burden, presence of cancer cells in lymph nodes, and distant metastasis, thus focusing mainly on tumor cells. During the equilibrium phase, which is typically the phase when the cancer is diagnosed in the patients, immune cells are still capable of eliminating tumor cells, but are unable to completely eradicate the tumor cells.
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| 3. |
- Pastula, Agnieszka, et al.
(författare)
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Induction of Epithelial-mesenchymal Transition in MDCK II Cells
- 2021
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Ingår i: Bio-protocol. - : Bio-protocol. - 2331-8325. ; 11:3
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Tidskriftsartikel (refereegranskat)abstract
- Epithelial-mesenchymal transition (EMT) is a reversible process of epithelial cell transdifferentiation into a mesenchymal cell, that enables initiation of cell migration. EMT plays an important role in embryonic development, tissue repair and cancer metastasis. Better understanding of cellular and molecular events during EMT will not only provide novel insights on how mammalian organism develops and how epithelial tissues regenerate, but also can identify novel therapeutic targets for cancer therapy. Here we aim to provide a detailed protocol on how to induce EMT in Madin-Darby Canine Kidney (MDCK) II epithelial cell line and perform immunofluorescent staining on EMT-induced cells.
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| 6. |
- Pastula, Agnieszka, et al.
(författare)
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Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts : A Method to Characterize the Gastrointestinal Stem Cell Niche
- 2016
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Ingår i: Stem Cells International. - : Hindawi Publishing Corporation. - 1687-9678 .- 1687-966X.
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Tidskriftsartikel (refereegranskat)abstract
- The gastrointestinal epithelium is characterized by a high turnover of cells and intestinal stem cells predominantly reside at the bottom of crypts and their progeny serve to maintain normal intestinal homeostasis. Accumulating evidence demonstrates the pivotal role of a niche surrounding intestinal stem cells in crypts, which consists of cellular and soluble components and creates an environment constantly influencing the fate of stem cells. Here we describe different 3D culture systems to culture gastrointestinal epithelium that should enable us to study the stem cell niche in vitro in the future: organoid culture and multilayered systems such as organotypic cell culture and culture of intestinal tissue fragments ex vivo. These methods mimic the in vivo situation in vitro by creating 3D culture conditions that reflect the physiological situation of intestinal crypts. Modifications of the composition of the culture media as well as coculturing epithelial organoids with previously described cellular components such as myofibroblasts, collagen, and neurons show the impact of the methods applied to investigate niche interactions in vitro. We further present a novel method to isolate labeled nerves from the enteric nervous system using Dclk1-CreGFP mice.
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