| 1. |
- Denysenko, A.P., et al.
(författare)
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A comprehensive study of dura mater biomineralization : morphological, crystallographic, and immunohistochemical aspects
- 2023
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Ingår i: Reports of Morphology. - : Vinnytsia National Pyrogov Memorial Medical University. - 1818-1295 .- 2616-6194. ; 29:4, s. 50-57
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Tidskriftsartikel (refereegranskat)abstract
- Biomineralization is a process of formation of biominerals widespread among living organisms. This phenomenon occurs in the central nervous system in normal and pathological conditions. Typically, this can manifest age-related changes, and the prevalence of biominerals increases with age. At the same time, it can be a sign of pathology-tumour growth, dystrophy, metabolic disorders, etc. This work aims to study the morphological features of the dura mater with signs of biomineralization. In this work, we examined 30 samples of the dura mater with signs of biomineralization (group I) and 30 samples without these signs (group II) obtained during autopsies in the pathology department of the Sumy Regional Clinical Hospital. For the morphological charact erist ics of the dura mat er, we used hi stologi cal, hi stochemi cal and immunohistochemical methods, as well as scanning electron microscopy. Statistical processing of the results of the immunohistochemical study was carried out in the GraphPad Prism 8.0 statistical package using parametric and non-parametric research methods. To divide the samples into groups, we relied on the histological method-staining with hematoxylin-eosin. Histochemical methods (alizarin red staining, von Koss method) confirmed the presence of calcium compounds in the studied group. Van Gieson staining visualized the collagen fibres of the dura mater, and the PAS reaction di d not reveal the presence of gl ycosaminogl ycans in the mineral deposit s. Immunohistochemical examination of the dura mater with antibodies against osteopontin revealed a significant difference between the study and control groups (p<0.001), confirming the critical role of osteopontin in the biomineralization process in this tissue. With the help of scanning electron microscopy, it was found that biominerals in the dura mater are represented by tens to hundreds of formations of various shapes (rounded, oval and irregular), varying from tens of nanometers to 50 micrometres. Their composition was analyzed using maps and spectra of energy-dispersive X-ray spectroscopy and most probably corresponded to calcium hydroxyapatite. So, the dura mater is characterized by biomineralization, manifested by the formation of microscopic calcium hydroxyapatite composites.
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| 2. |
- Krlin, L., et al.
(författare)
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Anomalous impurity diffusion in models of tokamak edge plasma turbulence
- 2004
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Ingår i: Czechoslovak Journal of Physics. - 0011-4626 .- 1572-9486. ; 54:1-3, s. 157-163
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Tidskriftsartikel (refereegranskat)abstract
- Using a simple spatially periodical and stationary potential as a model of electrostatic tokamak edge plasma turbulence, we have found recently [1, 2] an anomalous impurity diffusion in this regime. In this contribution, we estimate this diffusion for a more general and realistic form of the potential, which is close to the Hasegawa-Wakatani (HW) potential. As an interesting consequence of the discussed dynamics, we present, possibility of radial electric field generation in the edge turbulence regime. This effect. might play a role in tokamak scenarios with transport barriers.
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| 3. |
- Michaëlsson, Erik, et al.
(författare)
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Myeloperoxidase Inhibition Reverses Biomarker Profiles Associated With Clinical Outcomes in HFpEF.
- 2023
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Ingår i: JACC. Heart failure. - 2213-1787. ; 11:7, s. 775-87
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Tidskriftsartikel (refereegranskat)abstract
- Systemic microvascular dysfunction and inflammation are postulated to play a pathophysiologic role in heart failure with preserved ejection fraction (HFpEF).This study aimed to identify biomarker profiles associated with clinical outcomes in HFpEF and investigate how inhibition of the neutrophil-derived reactive oxygen species-producing enzyme, myeloperoxidase, affects these biomarkers.Using supervised principal component analyses, the investigators assessed the associations between baseline plasma proteomic Olink biomarkers and clinical outcomes in 3 independent observational HFpEF cohorts (n=86, n=216, and n=242). These profiles were then compared with the biomarker profiles discriminating patients treated with active drug vs placebo in SATELLITE (Safety and Tolerability Study of AZD4831 in Patients With HeartFailure), a double-blind randomized 3-month trial evaluating safety and tolerability of the myeloperoxidase inhibitor AZD4831 in HFpEF (n=41). Pathophysiological pathways were inferred from the biomarker profiles by interrogation of the Ingenuity Knowledge database.TNF-R1, TRAIL-R2, GDF15, U-PAR, and ADM were the top individual biomarkers associated with heart failure hospitalization or death, and FABP4, HGF, RARRES2, CSTB, and FGF23 were associated with lower functional capacity and poorer quality of life. AZD4831 downregulated many markers (most significantly CDCP1, PRELP, CX3CL1, LIFR, VSIG2). There was remarkable consistency among pathways associated with clinical outcomes in the observational HFpEF cohorts, the top canonical pathways being associated with tumor microenvironments, wound healing signaling, and cardiac hypertrophy signaling. These pathways were predicted to be downregulated in AZD4831 relative to placebo-treated patients.Biomarker pathways that were most strongly associated with clinical outcomes were also the ones reduced by AZD4831. These results support the further investigation of myeloperoxidase inhibition in HFpEF.
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| 6. |
- Panek, R., et al.
(författare)
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Anomalous ion diffusion and radial-electric-field generation in a turbulent edge plasma potential weakly correlated in time and space
- 2005
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Ingår i: Physica Scripta. - 0031-8949 .- 1402-4896. ; 72:4, s. 327-332
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Tidskriftsartikel (refereegranskat)abstract
- Using a simple model of tokamak-edge-plasma turbulence in the form of a spatially periodic and time-independent electrostatic potential, we have found recently a new type of anomalous impurity diffusion in this regime [1], [2]. In the present contribution, we estimate this diffusion in the test-particle approach for a real turbulent potential obtained in an experiment. A significant difference in the impurity dynannics between the Hamiltonian and drift approaches is observed. As an interesting consequence of these dynamics, the possibility of radial-electric-field generation in the turbulence regimes appears. This effect can be of importance in scenarios related to transport barriers.
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