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- Dahlman-Höglund, Anna, 1964, et al.
(author)
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Antibodies given orally in the neonatal period can affect the immune response for two generations: evidence for active maternal influence on the newborn's immune system.
- 1999
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In: Scandinavian journal of immunology. - 0300-9475. ; 50:6, s. 651-6
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Journal article (peer-reviewed)abstract
- Two day old Wistar rats were tube fed with 1 or 10 micrograms of a mouse IgG1 monoclonal anti-idiotypic (a-Id) antibody that was directed against an anti-Escherichia coli-K13 capsular polysaccharide antibody. A control group was given 10 micrograms of an unrelated control antibody. Six weeks after the administration of antibodies, the rats were intestinally colonised with an ovalbumin (OVA)-producing E. coli O6K13 strain. At 8 weeks of age, the male rats (first generation) and the offsprings of the female rats (second generation), were parenterally immunised with OVA and dead wild type E. coli O6K13, and the immune response was followed. In the rats of the first generation, there were no major differences between the groups in the immune response to the bacterium. However, the offspring of the neonatally a-Id administered rats had a profoundly affected immune response to the idiotypically connected antigen K13, but also to other antigens on the bacteria. Thus, a-Id treatment in the first generation gave, in the second generation, a greatly enhanced serum antibody response to the spatially related antigens OVA and O6 LPS, as well as to the idiotypically connected antigen K13. Concurrently, the in vitro spleen cell proliferative response to both OVA and the wild type bacterium was lowered. Overall, greater effects were seen with the higher dose of a-Id. In conclusion, our results demonstrate that by giving monoclonal antibodies idiotypically connected to a single bacterial component to neonatal rats, one profoundly influence the immune response also to other-spatially related-bacterial antigens in their offsprings.
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| 2. |
- Gjertsson, Inger, 1962, et al.
(author)
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Impact of transcription factors AP-1 and NF-kappaB on the outcome of experimental Staphylococcus aureus arthritis and sepsis.
- 2001
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In: Microbes and infection. - 1286-4579. ; 3:7, s. 527-34
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Journal article (peer-reviewed)abstract
- Staphylococcus aureus infection is, despite adequate antibiotic treatment, a disease characterized by high mortality. The bacterium triggers an exaggerated immune response in the host, which on the one hand acts as an efficient defense, but on the other hand gives rise to tissue damage. In this study we have modulated the hosts response to S. aureus by inhibition of nuclear factor kappaB (NF-kappaB) and activator protein-1 (AP-1)-triggered release of pro-inflammatory cytokines and tissue-destructive proteins, respectively. Mice were administered with antisense oligonucleotides (ODN) to the p65 subunit of NF-kappaB and/or a double-stranded oligonucleotide (mCoAP-1) with homology to the murine AP-1 binding site of collagenase IV gene (metalloproteinase-9; MMP-9), solely or in combination with antibiotics. In mice systemically treated with antisense ODN to NF-kappaB p65 alone, the bacterial burden in the kidneys was significantly increased (P = 0.04) The same tendency was seen when mCoAP-1 was administered either alone or in combination with antibiotics. We also found significantly (P = 0.04) elevated levels of IL-6 in p65 antisense treated mice. Surprisingly, this p65 antisense therapy approach, which has turned out to be highly efficient in amelioration of aseptic arthritis and colitis, failed to change the clinical course of either septic arthritis or sepsis. We suggest that interaction with transcription factors leads to increased bacterial burden in vivo, abrogating the potential benefits of the anti-inflammatory properties exerted by these compounds.
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| 3. |
- Karlsson, Malin, et al.
(author)
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"Tolerosomes” are produced by intestinal epithelial cells
- 2001
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In: European Journal of Immunology. ; 31, s. 2892-2900
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Journal article (peer-reviewed)abstract
- The development of immunological tolerance to orally fed antigens depends on the sampling, processing and transportation events followed in the intestinal epithelium. We present here a description of a ’’tolerosome’’: a supra-molecular, exosome-like structure assembled in and released from the small intestinal epithelial cell. The tolerosome is a e 40 nm large vesicular structure that carries MHC class II (MHC II) with bound antigenic peptides sampled from the gut lumen. Tolerosomes isolated from serum shortly after antigen feeding or from an in vitro pulsed intestinal epithelial cell line are fully capable of inducing antigen specific tolerance in naive recipient animals. Purified tolerosomes represent a structure by which fed antigens can be efficiently presented to the immune system. Removal of the tolerosomes from serum by ultracentrifugation or absorption of MHC II results in abrogated tolerance development.
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| 4. |
- Pettersson, Inger, 1962, et al.
(author)
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Natural (ghrelin) and synthetic (hexarelin) GH secretagogues stimulate H9c2 cardiomyocyte cell proliferation.
- 2002
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In: The Journal of endocrinology. - 0022-0795. ; 175:1, s. 201-9
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Journal article (peer-reviewed)abstract
- Recent experimental data demonstrate cardiovascular effects of the GH secretagogues (GHSs) hexarelin and ghrelin, the proposed natural ligand for the GHS receptor. Moreover, specific cardiac binding sites for GHSs have been suggested. The aim of the present study was to investigate if the natural ligand ghrelin and synthetic GHS peptide hexarelin and analogues have direct effects on the cardiomyocyte cell line, H9c2. Hexarelin stimulated thymidine incorporation in a dose-dependent manner with significant responses at 3 micro M (147+/-3% of control, P<0.01) and elicited maximal effects at concentrations around 30 micro M. This activity was seen already after 12 h of incubation with a maximal effect after 18 h (176+/-9% of control, P<0.01). Ghrelin also had a significant stimulatory effect on thymidine incorporation (129+/-2% of control at 3 micro M and 18 h, P<0.05). The stimulatory effect on thymidine incorporation of hexarelin, Tyr-Ala-hexarelin, EP80317 and ghrelin was specific and no stimulatory effect was observed with the truncated GH-releasing peptide EP51389 or the non-peptidyl GHS MK-0677. In competitive binding studies, (125)I-labeled Tyr-Ala-hexarelin was used as radioligand and competition curves showed displacement with hexarelin, Tyr-Ala-hexarelin, EP80317 and ghrelin, whereas MK-0677 and EP51389 produced very little displacement at 1 micro M concentration, adding further support for an alternative subtype binding site in the heart compared with the pituitary. In conclusion, we have demonstrated a dose-dependent and specific stimulation of cardiomyocyte thymidine incorporation by natural and synthetic GHS analogues, suggesting increased cell proliferation and binding of GHS to H9c2 cardiomyocyte cell membranes. These findings support potential peripheral effects of GHS on the cardiovascular system independent of an increased GH secretion.
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| 5. |
- Sultán Sjöqvist, Madeleine, 1962-
(author)
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"Vi blev muslimer" Svenska kvinnor berättar : En religionssociologisk studie av konversionsberättelser
- 2006
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Doctoral thesis (other academic/artistic)abstract
- Materialet i studien består av kvalitativa intervjuer med svenska kvinnor som konverterat till islam. I intervjuerna har frågor ställts om hur de uppfattar sitt religiösa engagemang och sitt liv som muslimer i ett västerländskt samhälle.I informanternas tolkning av sin konversion och sin nya religiösa tillhörighet har beskrivningar av konversionen som en process en central ställning. Denna process analyseras utifrån följande tre teman: Möte med islam och muslimer, jämförelse mellan ”det gamla” och ”det nya” sammanhanget samt konversion. Huvuddelen i avhandlingen består i att beskriva och tolka informanternas förståelse av denna process i relation till religionssociologiska frågeställningar.När informanterna tolkar och beskriver sitt religiösa engagemang är föreställningar om muslimskt familjeliv tätt knutna till förståelsen av vad det religiösa engagemanget innebär. Informanterna poängterar särskilt att islam står för jämlikhet mellan människor i allmänhet och mellan könen i synnerhet, att islam står för det goda patriarkala familjelivet och att kvinnor skall lyda sin make. Således är en del i avhandlingen vikt för en analys av informanternas förståelse av muslimskt familjeliv, kvinnors inflytande och antagonism mellan könen.Studiens teoretiska sammanhang är religionssociologiskt och könsteoretiskt. I avhandlingen är intervjumaterialet relaterat till fem sammanhang: religion som meningssystem, konversionsberättelser, kön som en social konstruktion, moderna och senmoderna samhällen samt religion i moderna och senmoderna samhällen.I avhandlingen är den religiositet som kommer till uttryck tolkat både som en del av och en reaktion mot det senmoderna livets villkor. I informanternas tolkningar ryms både öppnare och mer reflexiva tolkningar av vad ett muslimskt engagemang innebär och en strävan mot de ”rätta” svaren, ett fastnaglande av vad kön, familj, samhälle och religion ”är”. Det finns en spänning mellan att ingå i vad som uppfattas vara ”rätt” muslimskt tradition och det religiösa engagemanget tolkat som ett kvinnoemancipatoriskt projekt. Därtill finns en spänning mellan att ingå i den institutionella religionen och att etablera en religiositet på ”kvinnliga” villkor.
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