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- Engskog, Mikael K R, et al.
(author)
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Metabolic profiling of epithelial ovarian cancer cell lines : evaluation of harvesting protocols for profiling using NMR spectroscopy
- 2015
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In: Bioanalysis. - : Future Science Ltd. - 1757-6180 .- 1757-6199. ; 7:2, s. 157-166
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Journal article (peer-reviewed)abstract
- BACKGROUND: Metabolic profiling represents a novel technology for analyzing tumor cells. Epithelial ovarian carcinoma has a low survival rate due to the development of aggressive and chemotherapy-resistant cells. A tailored and reliable protocol is presented for profiling of chemoresistant cells using the cell line SKOV3 and a multiresistant subline SKOV3R.RESULTS: Harvesting protocols with cold methanol or MilliQ freeze/thaw cycles were compared. Increased reproducibility using MilliQ was evidenced. Importantly, both approaches resulted in similar profiles. Compared with parental SKOV3, the SKOV3R cells showed a significantly different profile.CONCLUSION: The MilliQ protocol is preferred owing to higher reproducibility and increased sample preparation options. The resulting metabolic profiles summarize metabolic alterations in chemoresistant cells consistent with a progressed and aggressive phenotype.
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| 4. |
- Pettersson, My, et al.
(author)
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Investigator self-interest in the environmental process
- 2015
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In: Nordic Environmental Law Journal. - 2000-4273. ; :2, s. 107-118
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Journal article (peer-reviewed)abstract
- The Swedish Environmental Code states that certain potentially harmful activities must not be pursued without a permit. When applying for a permit,the applicant shall submit an environmental impact assessment, which describes the effects that the activity might have on the environment. This article discusses the risk that investigator self-interest decreases the adequacy of environmental impact assessments. The article also presents a newly made empirical study of whether and how arguments about investigator self-interest are considered and taken on board by Swedish environmental courts.
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| 5. |
- Pettersson, My
(author)
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Trovärdighetens dimensioner
- 2017
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In: Tidsskrift for Rettsvitenskap. - : Scandinavian University Press / Universitetsforlaget AS. - 0040-7143 .- 1504-3096. ; 130:1
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Journal article (peer-reviewed)
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| 7. |
- Ramachandraiah, Harisha, et al.
(author)
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Bio-functionalized recombinant spider silk modified microdevice for capture and release of circulating tumor cells from pancreatic cancer patients
- 2020
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In: 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. - : Chemical and Biological Microsystems Society. ; , s. 862-863
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Conference paper (peer-reviewed)abstract
- Effective isolation and purification of circulating tumor cells from whole blood provides important capability for clinical application and biological research. Here, we demonstrate a single step surface modification procedure for a microfluidic device based on self-assembly of recombinant spider silk harbouring an affinity domain for antibody binding. Moreover, a proteolytic domain for enzymatic digestion of silk for effective release of cancer cells from whole blood of Pancreatic patient sample and the released cells are readily available for downstream processing.
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| 8. |
- Ramachandraiah, Harisha, et al.
(author)
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Microfluidic based circulating tumor cell isolation and release from whole blood of pancreatic cancer patients using bio-functionalized recombinant spider silk
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Other publication (other academic/artistic)abstract
- A bio-functionalized microsystem was developed for the capture and release of cancer cells from whole blood. Effective isolation and purification of circulating tumor cells from whole blood provides important capability for clinical application and biological research. Here, we demonstrate a single step surface modification procedure for a microfluidic device based on self-assembly of recombinant spider silk harbouring an affinity domain for antibody binding. The surfaces of microfluidic devices were conjugated/equipped with anti-EpCAM antibody for selective isolation of pancreatic cancer cells from spiked whole blood and finally circulating tumor cells from pancreatic cancer patients. Moreover, a protease-cleavage site in the recombinant spider silk proteins provides the unique option to release the captured cancer cells on command from the device without compromising the cell’s viability. Our approach offers a simple, easy and robust surface modification process with a 85% cancer cell capture efficiency. Subsequent addition of a site-specific protease results in the release of 95% of captured cells from the bio functionalized microfluidic systems.
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