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Search: WFRF:(Portoso M)

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  • Djupedal, Ingela, et al. (author)
  • RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing
  • 2005
  • In: Genes & Development. - : Cold Spring Harbor Laboratory. - 0890-9369 .- 1549-5477. ; 19:19, s. 2301-2306
  • Journal article (peer-reviewed)abstract
    • Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2 subunits of RNA polymerase II (RNA Pol II) were shown to mediate RNA interference (RNAi)-directed chromatin modification but did not affect pre-siRNA levels. Here we show that another Pol II subunit, Rpb7 has a specific role in presiRNA transcription. We define a centromeric presiRNA promoter from which initiation is exquisitely sensitive to the rpb7-G150D mutation. In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Ekwall, Karl (1)
Bonilla, Carolina (1)
Spåhr, H (1)
Djupedal, Ingela (1)
Portoso, M (1)
Gustafsson, C M (1)
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Allshire, R C (1)
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University
Södertörn University (1)
Karolinska Institutet (1)
Language
English (1)
Research subject (UKÄ/SCB)
Natural sciences (1)
Year
2005 (1)

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