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1.	Mitchell, Megan, I, et al. (author) Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER) : A customizable purification assay designed for small-RNA biomarker identification and evaluation of circulating small-EVs 2021 In: Journal of Extracellular Vesicles. - : John Wiley & Sons. - 2001-3078. ; 10:8 Journal article (peer-reviewed)abstract Circulating nucleic acids, encapsulated within small extracellular vesicles (EVs), provide a remote cellular snapshot of biomarkers derived from diseased tissues, however selective isolation is critical. Current laboratory-based purification techniques rely on the physical properties of small-EVs rather than their inherited cellular fingerprints. We established a highly-selective purification assay, termed EV-CATCHER, initially designed for high-throughput analysis of low-abundance small-RNA cargos by next-generation sequencing. We demonstrated its selectivity by specifically isolating and sequencing small-RNAs from mouse small-EVs spiked into human plasma. Western blotting, nanoparticle tracking, and transmission electron microscopy were used to validate and quantify the capture and release of intact small-EVs. As proof-of-principle for sensitive detection of circulating miRNAs, we compared small-RNA sequencing data from a subset of small-EVs serum-purified with EV-CATCHER to data from whole serum, using samples from a small cohort of recently hospitalized Covid-19 patients. We identified and validated, only in small-EVs, hsa-miR-146a and hsa-miR-126-3p to be significantly downregulated with disease severity. Separately, using convalescent sera from recovered Covid-19 patients with high anti-spike IgG titers, we confirmed the neutralizing properties, against SARS-CoV-2 in vitro, of a subset of small-EVs serum-purified by EV-CATCHER, as initially observed with ultracentrifuged small-EVs. Altogether our data highlight the sensitivity and versatility of EV-CATCHER.
2.	Santangelo, James S., et al. (author) Global urban environmental change drives adaptation in white clover 2022 In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 375 Journal article (peer-reviewed)abstract Urbanization transforms environments in ways that alter biological evolution. We examined whether urban environmental change drives parallel evolution by sampling 110,019 white clover plants from 6169 populations in 160 cities globally. Plants were assayed for a Mendelian antiherbivore defense that also affects tolerance to abiotic stressors. Urban-rural gradients were associated with the evolution of clines in defense in 47% of cities throughout the world. Variation in the strength of clines was explained by environmental changes in drought stress and vegetation cover that varied among cities. Sequencing 2074 genomes from 26 cities revealed that the evolution of urban-rural dines was best explained by adaptive evolution, but the degree of parallel adaptation varied among cities. Our results demonstrate that urbanization leads to adaptation at a global scale.

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Type of publication: journal article (2)

Type of content: peer-reviewed (2)

Author/Editor: Tack, Ayco J. M. (1); Doulabi, Ehsan Manou ... (1); Kamali-Moghaddam, Ma ... (1); Alberti, Marina (1); Pinho, Pedro (1); Puentes, Adriana (1); show more...; Laine, Anna Liisa (1); Bonte, Dries (1); del Val, Ek (1); Branquinho, Cristina (1); Peres-Neto, Pedro R. (1); Granath, Gustaf (1); Crawford, Andrew J. (1); Zhao, Zhigang (1); Classen, Aimée T. (1); Sanders, Nathan J. (1); Ellers, Jacintha (1); Raeymaekers, Joost A ... (1); Brans, Kristien, I (1); Gagnon, Edeline (1); Scheepens, J.F. (1); Parachnowitsch, Amy ... (1); Santangelo, James S. (1); Ness, Rob W. (1); Angeoletto, Fabio (1); Anstett, Daniel N. (1); Anstett, Julia (1); Baena-Diaz, Fernanda (1); Carlen, Elizabeth J. (1); Chaves, Jaime A. (1); Comerford, Mattheau ... (1); Dyson, Karen (1); Falahati-Anbaran, Mo ... (1); Fellowes, Mark D. E. (1); Hodgins, Kathryn A. (1); Iñiguez-Armijos, Car ... (1); Lázaro-Lobo, Adrián (1); Moles, Angela T. (1); Munshi-South, Jason (1); Paule, Juraj (1); Porth, Ilga M. (1); Santiago-Rosario, Lu ... (1); Whitney, Kaitlin Sta ... (1); Johnson, Marc T. J. (1); Rivas-Torres, Gonzal ... (1); Anderson, Jill T. (1); Dominoni, Davide M. (1); Szulkin, Marta (1); DiLeo, Michelle F. (1); Gkelis, Spyros (1); show less...

University: Uppsala University (2); Stockholm University (1); Lund University (1); Swedish University of Agricultural Sciences (1)

Language: English (2)

Research subject (UKÄ/SCB): Natural sciences (1); Medical and Health Sciences (1)

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