| 1. |
- Bengtsson, Anders A., et al.
(author)
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Metabolic Profiling of Systemic Lupus Erythematosus and Comparison with Primary Sjögren’s Syndrome and Systemic Sclerosis
- 2016
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In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 11:7
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Journal article (peer-reviewed)abstract
- Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease which can affect most organ systems including skin, joints and the kidney. Clinically, SLE is a heterogeneous disease and shares features of several other rheumatic diseases, in particular primary Sjögrens syndrome (pSS) and systemic sclerosis (SSc), why it is difficult to diag- nose The pathogenesis of SLE is not completely understood, partly due to the heterogeneity of the disease. This study demonstrates that metabolomics can be used as a tool for improved diagnosis of SLE compared to other similar autoimmune diseases. We observed differences in metabolic profiles with a classification specificity above 67% in the comparison of SLE with pSS, SSc and a matched group of healthy individuals. Selected metabolites were also significantly different between studied diseases. Biochemical pathway analysis was conducted to gain understanding of underlying pathways involved in the SLE pathogenesis. We found an increased oxidative activity in SLE, supported by increased xanthine oxidase activity and an increased turnover in the urea cycle. The most discriminatory metabolite observed was tryptophan, with decreased levels in SLE patients compared to control groups. Changes of tryptophan levels were related to changes in the activity of the aromatic amino acid decarboxylase (AADC) and/or to activation of the kynurenine pathway.
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| 2. |
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| 3. |
- Botero Vega, Carlos Alberto, et al.
(author)
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Additive Manufacturing of a Cold-Work Tool Steel using Electron Beam Melting
- 2020
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In: Steel Research International. - : Wiley. - 1611-3683 .- 1869-344X. ; 19:5, s. 1-6
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Journal article (peer-reviewed)abstract
- Metal additive manufacturing (AM) is on its way to industrialization. One of the most promising techniques within this field, electron beam melting (EBM), is nowadays used mostly for the fabrication of high‐performance Ti‐based alloy components for the aerospace and medical industry. Among the industrial applications envisioned for the future of EBM, the fabrication of high carbon steels for the tooling industry is of great interest. In this context, the process windows for dense and crack‐free specimens for a highly alloyed (Cr–Mo–V) cold‐work steel powder are presented in this article. High‐solidification rates during EBM processing lead to very fine and homogeneous microstructures. The influence of process parameters on the resulting microstructure and the chemical composition is investigated. In addition, preliminary results show very promising mechanical properties regarding the as‐built and heat‐treated microstructure of the obtained material.
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| 4. |
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| 5. |
- Eliasson, Mattias, et al.
(author)
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From data processing to multivariate validation : essential steps in extracting interpretable information from metabolomics data
- 2011
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In: Current Pharmaceutical Biotechnology. - : Bentham Science Publishers. - 1389-2010 .- 1873-4316. ; 12:7, s. 996-1004
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Research review (peer-reviewed)abstract
- In metabolomics studies there is a clear increase of data. This indicates the necessity of both having a battery of suitable analysis methods and validation procedures able to handle large amounts of data. In this review, an overview of the metabolomics data processing pipeline is presented. A selection of recently developed and most cited data processing methods is discussed. In addition, commonly used chemometric and machine learning analysis methods as well as validation approaches are described.
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| 6. |
- Eliasson, Mattias, et al.
(author)
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Strategy for optimizing LC-MS data processing in Metabolomics : A design of experiments approach
- 2012
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In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 84:15, s. 6869-6876
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Journal article (peer-reviewed)abstract
- A strategy for optimizing LC-MS metabolomics data processing is proposed. We applied this strategy on the XCMS open source package written in R on both human and plant biology data. The strategy is a sequential design of experiments (DoE) based on a dilution series from a pooled sample and a measure of correlation between diluted concentrations and integrated peak areas. The reliability index metric, used to define peak quality, simultaneously favors reliable peaks and disfavors unreliable peaks using a weighted ratio between peaks with high and low response linearity. DoE optimization resulted in the case studies in more than 57% improvement in the reliability index compared to the use of the default settings. The proposed strategy can be applied to any other data processing software involving parameters to be tuned, e.g., MZmine 2. It can also be fully automated and used as a module in a complete metabolomics data processing pipeline.
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| 7. |
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| 8. |
- Mellin, P., et al.
(author)
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Bonding EBM-built blocks of 316L steel, using hot isostatic pressing
- 2017
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In: Proceedings Euro PM 2017: International Powder Metallurgy Congress and Exhibition. - : European Powder Metallurgy Association (EPMA).
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Conference paper (peer-reviewed)abstract
- To enable production of the First Wall Beam in the ITER fusion reactor; we attempt to join EBM-built blocks of 316L, by Hot Isostatic Pressing (HIP). For highly critical components, EBM-built material is usually HIPed anyway to heal defects such as pores and cracks. Using HIP to simultaneously bond several print-jobs together into a larger component saves time and reduces manufacturing complexity. We found by carrying out this research, that fine surface roughness is an important enabler for a complete bond. The raw printed surfaces that are obtained straight from the Arcam machine (Ra = 19.2 μm) do not enable a good bond. Instead, traditional machining, which in this paper reached Ra = 2.0 μm, enabled a good bond. HIP parameters are also important. The best bond in this study was achieved after increasing holding time from 1 h to 2 h.
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| 9. |
- Roos, Stefan, 1983-, et al.
(author)
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Assessing the viability of high-frequency spot melting for super duplex stainless steel 2507 via electron beam powder bed fusion
- 2023
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In: Journal of Materials Research and Technology. - : Elsevier BV. - 2238-7854. ; 27, s. 5720-5728
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Journal article (peer-reviewed)abstract
- This study investigates the use of Electron Beam Powder Bed Fusion (PBF-EB) spot melting on SDSS 2507, a material known for its high tensile strength, corrosion resistance, and welding properties. Spot melting, a localized melting technique, utilize a stationary beam to create melt pools before rapidly repositioning to form new ones, offering precise control over material properties in additive manufacturing. We conducted a design of experiments with 32 samples and analysed them using SEM, XRD, EBSD, optical microscopy, nanoindentation and statistical modelling. Elevated PBF-EB processing temperatures significantly influence microstructure and phase composition. XRD analysis identified the presence of the detrimental sigma phase. EBSD analysis revealed a composition primarily consisting of austenite (63 %) and sigma phase (33.5 %), with residual ferrite (3.5 %). Statistical modelling demonstrated that a combination of spot-time, spot distance, focus offset, and layer thickness was the most reliable predictor for density while area energy proved to be the most accurate predictor for hardness, with R2adj values of 0.766 and 0.802, respectively. Our study confirms that PBF-EB is capable of processing SDSS 2507 through spot melting, resulting in high-density samples at high productivity rates. The presence of sigma phase shows a need for post build heat treatment to achieve the desired phase distribution. This research enhances the understanding of the process and also holds promise for industrial applications.
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| 10. |
- Roos, Stefan, et al.
(author)
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Characterization of 316ln lattice structures fabricated via electron beam melting
- 2017
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In: Materials Science and Technology Conference and Exhibition 2017, MS and T 2017. - : Association for Iron and Steel Technology, AISTECH. - 9781510850583 ; , s. 336-343
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Conference paper (peer-reviewed)abstract
- One of the promising application areas of additive manufacturing (AM) relates to light weight structures, including complex near net shape geometries and lattices. So far one of the limiting factors hampering wider industrial usage of AM technologies is the limited availability of processed materials. The aim of present study was to expand the previous success in electron beam melting (EBM®) manufacturing of 316LN bulk materials into thinner lattice structures thus further widening the application areas available for the method. Present paper reports on the initial results where lattice structures with octagonal basic cells were manufactured using EBM® and characterized using microscopy and compression testing.
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