| 1. |
- Rahman, Mokhlasur, et al.
(author)
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Persistence, transmission, and virulence characteristics of Aeromonas strains in a duckweed aquaculture-based hospital sewage water recycling plant in Bangladesh
- 2007
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In: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 73:5, s. 1444-1451
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Journal article (peer-reviewed)abstract
- The persistence and transmission of Aeromonas in a duckweed aquaculture-based hospital sewage water treatment plant in Bangladesh was studied. A total of 670 samples from different sites of the hospital sewage water treatment plant, from feces of hospitalized children suffering from diarrhea, from environmental control ponds, and from feces of healthy humans were collected over a period of three years. In total, 1,315 presumptive Aeromonas isolates were biochemically typed by the PhenePlate rapid screening system (PhP-AE). A selection of 90 representative isolates was further analyzed with PhenePlate (PhP) extended typing (PhP-48), fatty acid methyl ester analysis, and amplified fragment length polymorphism (AFLP) fingerprinting. In addition, the prevalence of the putative virulence factors hemolysin and cytotoxin and the presence of the cytolytic enterotoxin gene (AHCYTOEN) were analyzed. Aeromonas was found at all sites of the treatment plant, in 40% of the samples from environmental control ponds, in 8.5% of the samples from hospitalized children suffering from diarrhea, and in 3.5% of samples from healthy humans. A significantly high number of Aeromonas bacteria was found in duckweed, which indicates that duckweed may serve as a reservoir for these bacteria. PhP-AE typing allowed identification of more than 192 distinct PhP types, of which 18 major PhP types (MTs) were found in multiple sites and during several occasions. AFLP fingerprinting revealed the prevalence of genotypically indistinguishable Aeromonas isolates among certain PhP MTs recovered from different sampling occasions and/or at multiple sites. Hemolytic and cytotoxic activities were observed in 43% of the tested strains, whereas 29% possessed the cytolytic enterotoxin gene AHCYTOEN. Collectively, two specific MTs associated with diarrhea were shown to exhibit high cytotoxicity. Furthermore, all tested isolates of these major types were positive for the cytolytic enterotoxin gene. In conclusion, our data indicate that certain phenotypically and genotypically stable clonal lineages of Aeromonas have persisted in the treatment system for a prolonged period and might spread from the hospitalized children suffering from diarrhea to fish produced for human consumption through the sewage water treatment system.
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| 2. |
- Mahmodul Hasan, Md., et al.
(author)
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D3mciAD : Data-Driven Diagnosis of Mild Cognitive Impairment Utilizing Syntactic Images Generation and Neural Nets
- 2021
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In: Brain Informatics. - Cham : Springer. ; , s. 366-377
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Conference paper (peer-reviewed)abstract
- Alzheimer’s disease, an incurable chronic neurological disorder (NLD) that affects human memory and demises cognitive thinking ability with shrinkage of the brain area. Early detection of Alzheimer’s disease (AD) is the only hope to delay its effect. This study designed a computer-aided automated detection method that can detect mild cognitive impairment for AD from magnetic resonance image scans. The data-driven solution approach requires an extensive quantity of annotated images for diagnosis. However, obtaining a large amount of annotated data for medical application is a challenging task. We have exploited a deep convolutional generative adversarial network (DCGAN) for synthesizing high-quality images to increase dataset size. A fine-tuned CNN (VGG16 architecture) model works on images to extract the intuitive features for early diagnosis. The extracted features of images by VGG16 feed into the support vector machine for classification. This research has conducted copious experiments to validate the proposed method outperformed relative baselines on public datasets.
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| 4. |
- Rahman, Motiur, et al.
(author)
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A Biotechnological Approach for the Production of Red Gerbera (Gerbera Jamesonii Bolus)
- 2014
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In: Nova Journal of Medical and Biological Sciences. - Canada : Nova Explore Publications. - 2292-793X .- 2292-793X. ; 2:1, s. 1-6
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Journal article (peer-reviewed)abstract
- An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved by culturing flower bud, leaf segments and flower stalk segments of 80 days old field grown plants on Murashige and Skoog (MS) medium supplemented with different concentration (1.0-6.0 mg/l) of 6-benzyl adenine (BA) in combination with single concentration (1.0 mg/l) of α-naphthalene acetic acid (NAA). Lower concentration of BA (1.0 and 2.0 mg/l) with NAA induced the explants to form callus. On the other hand when the explants were cultured in higher concentration (5.0 mg/l) of BA produced shoots and 5.0 mg/l BA with 1.0 mg/l NAA was found to be the best for shoot proliferation of the three explants optimum response was obtained from flower buds. Further multiplication of shoots occurred upon transfer of shoot clumps to BA containing MS medium. Regenerated shoots were rooted in MS medium with indole-3-acetic acid (IAA) or indole-3-buteric acid (IBA) and maximum frequency (81%) of rooting with highest number (4) of roots per shoot was achieved in MS medium fortified with 0.3 mg/l IBA. The rooted shoots were acclimatized and successfully established in soil under natural environment with maximum 84% survivability.
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| 5. |
- Rahman, Motiur
(author)
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Characterization of surface components of Moraxella catarrhalis and pathogenic Neisseria
- 1997
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Doctoral thesis (other academic/artistic)abstract
- Infection is a multistep process including adhesion, colonisation, invasion and multiplication which finally results in disease. Bacterial surface components and virulence factors are essential for all these different steps and they are co-ordinately regulated during the different steps of pathogenesis and infection. Host factors like the mucosal barrier, complement, phagocytosis, humoral and cellular immune response result in either elimination of pathogen or, if they fail to protect the body, progress of the disease process. LPS is an indispensable surface component and important virulence factor of gram- negative bacteria. The antibody response against different LPS serotypes of M. catarrhalis was studied in hyperimmune rabbit sera and a predominant serotype specific antibody response was observed. On the other hand, in the human host, humoral immune response was mainly observed against the common inner core region of the LPS. Immunochemical characterization of LPS epitopes by monoclonal antibodies confirmed the presence of a Gal-alpha-(1-4)-Gal epitope in the LPS of M. catarrhalis which is in agreement with the chemical structure of M. catarrhalis LPS. The humoral immune response against major outer membrane proteins was also studied in paired sera from patients suffering from a respiratory tract infection caused by M. catarrhalis. Titre rise in specific IgG 1, IgG2 and IgG3 was observed in most of the patients. Optimal sensitivity was obtained by using the OMP as antigen and testing for a rise of IgG3 antibodies. A good correlation was observed between total immunoglobulin response and IgG3 response. PilC, a 110 kD pilus associated protein was studied for function and localization. PilC is expressed from the pilC locus and N. meningitidis FAM20 expresses two PilC proteins, PilC 1 and PilC2 encoded by pilC1 and pilC2. PilC 1 and pilC2 are highly homologous but not identical. PilC1 and PilC2 share 84.7% similarity and 74.5% identity. PilC1 or PilC2 or both are essential for pilus biogenesis since pilC1-, pilC2- mutants were nonpiliated. In N. meningitidis, expression of PilC1, but not PilC2, contributes to attachment to host cells. Immunogold electron microscopy of cryosections and whole piliated bacteria indicated that PilC is located in the outer membrane and at the base of the pili.
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