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  • Beal, Jacob, et al. (author)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Journal article (peer-reviewed)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Reed, Mark, et al. (author)
  • Policy and Governance Options for Peatlands Conservation, Restoration and Sustainable Management
  • 2022
  • In: Global Peatlands Assessment. - : United Nations Environment Programme. - 9789280739916 ; , s. 252-271
  • Book chapter (peer-reviewed)abstract
    • In an era of high uncertainty, rapid environmental change, and increased recognition of the coupling of social and ecological systems, it is clear that there are limitations to current peatland policy and governance. As policy goals, and biophysical, social and political conditions differ between locations, the governance of peatlands needs to be tailored to each national, regional, and local context. This governance must also be adjusted for the needs and interests of the human populations and other species that depend upon these habitats. The adoption of gender-responsive approaches that take into account the needs of both women and men especially those from lower socioeconomic status, are thus crucial if we are to make progress towards environmental sustainability.This chapter reviews a range of policy and governance instruments that can halt further degradation and destruction of peatlands and facilitate their conservation, restoration and sustainable management. 
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