| 1. |
- Rehnstam-Holm, Ann-Sofi, et al.
(author)
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Forskningsanknuten grundutbildning : utbildningsanknuten forskning?
- 2018
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In: Man and Biosphere Health. - Kristianstad : Högskolan Kristianstad. ; , s. 8-15
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Book chapter (other academic/artistic)abstract
- Begreppet forskningsanknuten utbildning kan definieras på ett flertal sätt. Idealt ska studenterna inom sin utbildning stöta på forskning genom att få kännedom om aktuell forskning och vara praktiskt involverade i forskningsprojekt. För att detta ska uppnås bör majoriteten av lärarna vara forskningsaktiva inom för utbildningen relevanta områden och att ett vetenskapligt förhållningssätt till kunskap förmedlar på bästa pedagogiska sätt också detta förankrat i forskning. I den här artikeln ger vi exempel på hur studenter har involverats i våra forskningsprojekt både på högskolan, på universitetssjukhuset i Lund och vid internationella universitet och forskningsstationer.
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| 2. |
- Kaur-Kahlon, G., et al.
(author)
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Response of a coastal tropical pelagic microbial community to changed salinity and temperature
- 2016
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In: Aquatic Microbial Ecology. - 0948-3055 .- 1616-1564. ; 77:1, s. 37-50
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Journal article (peer-reviewed)abstract
- Studies on the responses of tropical microbial communities to changing hydrographic conditions are presently poorly represented. We present here the results from a mesocosm experiment conducted in southwest (SW) coastal India to investigate how changes in temperature and salinity may affect a coastal tropic microbial community. The onset of algal and bacterial blooms, the maximum production and biomass, and the interrelation between phytoplankton and bacteria were studied in replicated mesocosms. The treatments were set up featuring ambient conditions (28 °C, 35 PSU), hyposalinity (31 PSU), warming (31 °C) and a double manipulated treatment with warming and hyposalinity (31 °C, 31 PSU). The hyposaline treatment had the most considerable influence manifested as significantly lower primary production, and the most dissimilar microphytoplankton species community. The increased temperature acted as a catalyst in the double manipulated treatment and higher primary production was maintained. We investigated the dynamics of the microbial community with a structural equation model approach, and found a significant interrelation between phytoplankton biomass and bacterial abundance. Using this methodology, it became evident that temperature and salinity changes, individually and together, mediate direct and indirect effects that influence different compartments of the microbial loop. In the face of climate change, we suggest that in relatively nutrient replete tropical coastal zones, salinity and temperature changes will affect nutrient assimilation with subsequent significant effects on the quantity of microbial biomass and production.
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| 3. |
- Hernroth, Bodil E., et al.
(author)
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Environmental factors influencing human viral pathogens and their potential indicator organisms in the blue mussel, Mytilus edulis : the first Scandinavian report
- 2002
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In: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 68:9, s. 4523-4533
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Journal article (peer-reviewed)abstract
- This study was carried out in order to investigate human enteric virus contaminants in mussels from three sites on the west coast of Sweden, representing a gradient of anthropogenic influence. Mussels were sampled monthly during the period from February 2000 to July 2001 and analyzed for adeno-, entero-, Norwalk-like, and hepatitis A viruses as well as the potential viral indicator organisms somatic coliphages, F-specific RNA bacteriophages, bacteriophages infecting Bacteroides fragilis, and Escherichia coli. The influence of environmental factors such as water temperature, salinity, and land runoff on the occurrence of these microbes was also included in this study. Enteric viruses were found in 50 to 60% of the mussel samples, and there were no pronounced differences between the samples from the three sites. E. coli counts exceeded the limit for category A for shellfish sanitary safety in 40% of the samples from the sites situated in fjords. However, at the site in the outer archipelago, this limit was exceeded only once, in March 2001, when extremely high levels of atypical indole-negative strains of E. coli were registered at all three sites. The environmental factors influenced the occurrence of viruses and phages differently, and therefore, it was hard to find a coexistence between them. This study shows that, for risk assessment, separate modeling should be done for every specific area, with special emphasis on environmental factors such as temperature and land runoff. The present standard for human fecal contamination, E. coli, seems to be an acceptable indicator of only local sanitary contamination; it is not a reliable indicator of viral contaminants in mussels. To protect consumers and get verification of "clean" mussels, it seems necessary to analyze for viruses as well. The use of a molecular index of the human contamination of Swedish shellfish underscores the need for reference laboratories with high-technology facilities.
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| 4. |
- Holm, Ingvar, et al.
(author)
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Miljö, medicin och undervisning, hur hänger det ihop? : exempel från projektet mangan och prostatacancer
- 2018
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In: Man and Biosphere Health. - Kristianstad : Högskolan Kristianstad. ; , s. 16-21
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Book chapter (other academic/artistic)abstract
- Forskningsmiljön ”Man and Biosphere Health” är engruppering där forskare från helt olika biologiskakunskapsområden träffas och knyter kontakter. Forskning inom området ”Life Science” (Livsvetenskap), som framförallt innefattar biologi, medicin och biokemi, är världens största tvärdisciplinära forskningsområde med studier av biologisktliv samt de förutsättningar som utgör grunden för fortsatt liv. Unikt för samarbetet inom MABH är kombinationen avekologisk och biomedicinsk kompetens, vilket i vårt fall har inneburit att cellbiologisk forskning har knutits ihop med miljöforskning på ett nyskapande sätt.
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| 7. |
- Asplund, Maria E., et al.
(author)
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Water column dynamics of Vibrio in relation to phytoplankton community composition and environmental conditions in a tropical coastal area
- 2011
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In: Environmental Microbiology. - 1462-2912 .- 1462-2920. ; 13:10, s. 2738-2751
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Journal article (peer-reviewed)abstract
- Vibrio abundance generally displays seasonal patterns. In temperate coastal areas, temperature and salinity influence Vibrio growth, whereas in tropical areas this pattern is not obvious. The present study assessed the dynamics of Vibrio in the Arabian Sea, 1-2 km off Mangalore on the south-west coast of India, during temporally separated periods. The two sampling periods were signified by oligotrophic conditions, and stable temperatures and salinity. Vibrio abundance was estimated by culture-independent techniques in relation to phytoplankton community composition and environmental variables. The results showed that the Vibrio density during December 2007 was 10- to 100-fold higher compared with the February-March 2008 period. High Vibrio abundance in December coincided with a diatom-dominated phytoplankton assemblage. A partial least squares (PLS) regression model indicated that diatom biomass was the primary predictor variable. Low nutrient levels suggested high water column turnover rate, which bacteria compensated for by using organic molecules leaking from phytoplankton. The abundance of potential Vibrio predators was low during both sampling periods; therefore it is suggested that resource supply from primary producers is more important than top-down control by predators.
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| 9. |
- Axelsson, Carolina, 1967-, et al.
(author)
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Optimization of several parameters in order to reduce time in antibiotic susceptibility testing in a clinical laboratory
- 2017
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Conference paper (other academic/artistic)abstract
- Background - When sepsis or bacteraemia is suspected, patient blood samples are cultivated in blood culture bottles and then further incubated for identification of the organism and antimicrobial susceptibility testing. These methods are slow, identifying causative pathogens in a couple of hours, and antibiotic susceptibility results within 18-36 hours.Here we present optimization of several parameters in order to evaluate if the MBT ASTRA™ method can be a rapid tool, used for routine antibiotics susceptibility testing, in a clinical laboratory.Methods – MALDI-TOF MS measurements were performed with a Microflex LT/SH bench-top mass spectrometer (Bruker) with standard settings. The resulting spectra were uploaded in the MBT-ASTRA™ software, which normalizes the peaks and determines the AUC and RG values for each setup.Results - The bacterial preparation steps generated a new protocol, which reduced time with 30-60 minutes.The antibiotics susceptibility test was optimized for 90 minutes incubation time. 200 µl McFarland 0.5 bacterial suspension in broth were incubated in broth at 37°C, with and without 32 µg/ml Cefotaxime, 16 µg/ml Meropenem and 4 µg/ml Ciprofloxacin.The suspensions were transferred to 0.45 µm pore size filter membraned 96 well plate. They were centrifuged; washed; fixated and eluted; put on a MALDI-target, and covered by matrix solution. All could be automated with robot, which reduced time with 60 minutes.Conclusion – Rapid susceptibility testing becomes more requested with the increase of resistance bacteria causing infections. Our study can be a valuable tool for clinical laboratories striving for reduction in time handling of antibiotic susceptibility testing.
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| 10. |
- Axelsson, Carolina, et al.
(author)
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Optimization of several parameters in order to reduce time in antibiotic susceptibility testing in a clinical laboratory
- 2017
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Conference paper (other academic/artistic)abstract
- Background - When sepsis or bacteraemia is suspected, patient blood samples are cultivated in blood culture bottles and then further incubated for identification of the organism and antimicrobial susceptibility testing. These methods are slow, identifying causative pathogens in a couple of hours, and antibiotic susceptibility results within 18-36 hours. Here we present optimization of several parameters in order to evaluate if the MBT ASTRA™ method can be a rapid tool, used for routine antibiotics susceptibility testing, in a clinical laboratory. Methods – MALDI-TOF MS measurements were performed with a Microflex LT/SH bench-top mass spectrometer (Bruker) with standard settings. The resulting spectra were uploaded in the MBT-ASTRA™ software, which normalizes the peaks and determines the AUC and RG values for each setup. Results - The bacterial preparation steps generated a new protocol, which reduced time with 30-60 minutes. The antibiotics susceptibility test was optimized for 90 minutes incubation time.200 µl McFarland 0.5 bacterial suspension in broth were incubated in broth at 37°C, with and without 32 µg/ml Cefotaxime, 16 µg/ml Meropenem and 4 µg/ml Ciprofloxacin. The suspensions were transferred to 0.45 µm pore size filter membraned 96 well plate. They were centrifuged; washed; fixated and eluted; put on a MALDI-target, and covered by matrix solution. All could be automated with robot, which reduced time with 60 minutes. Conclusion – Rapid susceptibility testing becomes more requested with the increase of resistance bacteria causing infections. Our study can be a valuable tool for clinical laboratories striving for reduction in time handling of antibiotic susceptibility testing.
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