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Träfflista för sökning "WFRF:(Rogar Marija) "

Search: WFRF:(Rogar Marija)

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  • Kolundzic, Nikola, et al. (author)
  • Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype
  • 2019
  • In: Stem Cell Research. - : ELSEVIER SCIENCE BV. - 1873-5061 .- 1876-7753. ; 37
  • Journal article (peer-reviewed)abstract
    • We have generated MLi002-A, a new induced pluripotent stem cell (iPSC) line derived from keratinocytes of a skin punch biopsy of a female patient with the severe epidermolysis bullosa simplex Dowling-Meara phenotype and the keratin K5 E475G mutation. Keratinocytes were reprogrammed using non-integrating Sendai virus vectors, and xeno-free culture conditions were used throughout. The characterization of MLi002-A cell line consisted of molecular karyotyping, mutation screening using restriction enzyme digestion and Sanger sequencing, and testing of the pluripotency and differentiation potentials by immunofluorescence of associated markers both in vitro and in vivo. This is the first iPSC model of EB Simplex.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Liovic, Mirjana (1)
Törmä, Hans, Profess ... (1)
Kolundzic, Nikola (1)
Khurana, Preeti (1)
Hobbs, Carl (1)
Rogar, Marija (1)
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Ropret, Sandra (1)
Ilic, Dusko (1)
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University
Uppsala University (1)
Language
English (1)
Research subject (UKÄ/SCB)
Medical and Health Sciences (1)
Year
2019 (1)

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