| 1. |
- Sotak, Matus, et al.
(author)
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Intestinal sodium/glucose cotransporter 3 expression is epithelial and downregulated in obesity.
- 2021
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In: Life sciences. - : Elsevier BV. - 1879-0631 .- 0024-3205. ; 267
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Journal article (peer-reviewed)abstract
- We aimed to determine whether the sodium/glucose cotransporter family member SGLT3, a proposed glucose sensor, is expressed in the intestine and/or kidney, and if its expression is altered in mouse models of obesity and in humans before and after weight-loss surgery.We used in-situ hybridization and quantitative PCR to determine whether the Sglt3 isoforms 3a and 3b were expressed in the intestine and kidney of C57, leptin-deficient ob/ob, and diabetic BTBR ob/ob mice. Western blotting and immunohistochemistry were also used to assess SGLT3 protein levels in jejunal biopsies from obese patients before and after weight-loss Roux-en-Y gastric bypass surgery (RYGB), and in lean healthy controls.Sglt3a/3b mRNA was detected in the small intestine (duodenum, jejunum and ileum), but not in the large intestine or kidneys of mice. Both isoforms were detected in epithelial cells (confirmed using intestinal organoids). Expression of Sglt3a/3b mRNA in duodenum and jejunum was significantly lower in ob/ob and BTBR ob/ob mice than in normal-weight littermates. Jejunal SGLT3 protein levels in aged obese patients before Roux-en-Y gastric bypass (RYGB) were lower than in lean individuals, but substantially upregulated 6months post-RYGB.Our study shows that Sglt3a/3b is expressed primarily in epithelial cells of the small intestine in mice. Furthermore, we observed an association between intestinal mRNA Sglt3a/3b expression and obesity in mice, and between jejunal SGLT3 protein levels and obesity in humans. Further studies are required to determine the possible role of SGLT3 in obesity.
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| 2. |
- Abdillahi, Suado M., et al.
(author)
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Collagen VI Contains Multiple Host Defense Peptides with Potent In Vivo Activity
- 2018
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In: Journal of Immunology. - : AMER ASSOC IMMUNOLOGISTS. - 0022-1767 .- 1550-6606. ; 201:3, s. 1007-1020
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Journal article (peer-reviewed)abstract
- Collagen VI is a ubiquitous extracellular matrix component that forms extensive microfibrillar networks in most connective tissues. In this study, we describe for the first time, to our knowledge, that the collagen VI von Willebrand factor type A like domains exhibit a broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria in human skin infections in vivo. In silico sequence and structural analysis of VWA domains revealed that they contain cationic and amphipathic peptide sequence motifs, which might explain the antimicrobial nature of collagen VI. In vitro and in vivo studies show that these peptides exhibited significant antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa through membrane disruption. Our findings shed new light on the role of collagen VI derived peptides in innate host defense and provide templates for development of peptide-based antibacterial therapies.
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| 3. |
- Dorshorst, Ben, et al.
(author)
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A Complex Genomic Rearrangement Involving the Endothelin 3 Locus Causes Dermal Hyperpigmentation in the Chicken
- 2011
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In: PLoS Genetics. - : Public Library of Science (PLoS). - 1553-7390 .- 1553-7404. ; 7:12, s. e1002412-
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Journal article (peer-reviewed)abstract
- Dermal hyperpigmentation or Fibromelanosis (FM) is one of the few examples of skin pigmentation phenotypes in the chicken, where most other pigmentation variants influence feather color and patterning. The Silkie chicken is the most widespread and well-studied breed displaying this phenotype. The presence of the dominant FM allele results in extensive pigmentation of the dermal layer of skin and the majority of internal connective tissue. Here we identify the causal mutation of FM as an inverted duplication and junction of two genomic regions separated by more than 400 kb in wild-type individuals. One of these duplicated regions contains endothelin 3 (EDN3), a gene with a known role in promoting melanoblast proliferation. We show that EDN3 expression is increased in the developing Silkie embryo during the time in which melanoblasts are migrating, and elevated levels of expression are maintained in the adult skin tissue. We have examined four different chicken breeds from both Asia and Europe displaying dermal hyperpigmentation and conclude that the same structural variant underlies this phenotype in all chicken breeds. This complex genomic rearrangement causing a specific monogenic trait in the chicken illustrates how novel mutations with major phenotypic effects have been reused during breed formation in domestic animals.
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| 4. |
- Englund, Thomas, et al.
(author)
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Relatedness and diversity of nine Swedish local chicken breeds as indicated by the mtDNA D-loop
- 2014
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In: Hereditas. - : Springer Science and Business Media LLC. - 0018-0661 .- 1601-5223. ; 151, s. 229-233
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Journal article (peer-reviewed)abstract
- In this study part of the mitochondrial D-loop was sequenced in a total of 40 samples from nine Swedish local chicken breeds. Among our 40 samples we observed 15 segregating sites and seven different haplotypes. The most common haplotype was present in all investigated individuals in fi ve breeds and together with other haplotypes in three breeds. This haplotype is common in domestic chickens and has been found in both local and commercial breeds in many parts of the world. The breed Ö landsh ö na was most different from the other Swedish breeds with all three individuals sharing a haplotype that differed from the most common haplotype at nine of the 15 segregating sites.
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| 5. |
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| 6. |
- Lelliott, Christopher J., et al.
(author)
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Ablation of PGC-1beta results in defective mitochondrial activity, thermogenesis, hepatic function, and cardiac performance
- 2006
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In: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 4:11
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Journal article (peer-reviewed)abstract
- The transcriptional coactivator peroxisome proliferator-activated receptor-gamma coactivator-1beta (PGC-1beta) has been implicated in important metabolic processes. A mouse lacking PGC-1beta (PGC1betaKO) was generated and phenotyped using physiological, molecular, and bioinformatic approaches. PGC1betaKO mice are generally viable and metabolically healthy. Using systems biology, we identified a general defect in the expression of genes involved in mitochondrial function and, specifically, the electron transport chain. This defect correlated with reduced mitochondrial volume fraction in soleus muscle and heart, but not brown adipose tissue (BAT). Under ambient temperature conditions, PGC-1beta ablation was partially compensated by up-regulation of PGC-1alpha in BAT and white adipose tissue (WAT) that lead to increased thermogenesis, reduced body weight, and reduced fat mass. Despite their decreased fat mass, PGC1betaKO mice had hypertrophic adipocytes in WAT. The thermogenic role of PGC-1beta was identified in thermoneutral and cold-adapted conditions by inadequate responses to norepinephrine injection. Furthermore, PGC1betaKO hearts showed a blunted chronotropic response to dobutamine stimulation, and isolated soleus muscle fibres from PGC1betaKO mice have impaired mitochondrial function. Lack of PGC-1beta also impaired hepatic lipid metabolism in response to acute high fat dietary loads, resulting in hepatic steatosis and reduced lipoprotein-associated triglyceride and cholesterol content. Altogether, our data suggest that PGC-1beta plays a general role in controlling basal mitochondrial function and also participates in tissue-specific adaptive responses during metabolic stress.
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| 8. |
- Parwal, Arvind, et al.
(author)
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Grid integration and a power quality assessment of a wave-energy park
- 2019
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In: IET Smart Grid. - UK : Institution of Engineering and Technology (IET). - 2515-2947. ; 2:4, s. 625-634
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Journal article (peer-reviewed)abstract
- This study presents a step toward the grid connection of a wave-energy park through an electric power conversion system (EPCS) developed and installed for the wave-energy harvesting in Lysekil, Sweden. The EPCS comprises a rectifier, a DC bus, and an inverter followed by a harmonic filter (HF). The higher- and lower-order harmonics injected by the inverter in a power quality context are investigated. The lower-order voltage harmonics partially distort the voltage-source inverter output grid current. A phase-locked loop-based (PLL) grid-phase tracking is used to attenuate the lower-order harmonics by reflecting the grid harmonics in the inverter output. An expression for the grid-current harmonics as a function of the grid-voltage harmonics has been derived and implemented. A mathematical model is derived to obtain a transfer function for the PLL, and finally, proportional–integral gains are tuned for stable system operation. An HF for mitigating the higher-order harmonics has been implemented. The total harmonic distortion is evaluated experimentally, and the results fulfil the grid-code requirements at various frequencies and harmonic orders.
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| 9. |
- Parwal, Arvind, et al.
(author)
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Wave Energy Research at Uppsala University and The Lysekil Research Site, Sweden : A Status Update
- 2015
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Conference paper (peer-reviewed)abstract
- This paper provides a summarized status update ofthe Lysekil wave power project. The Lysekil project is coordinatedby the Div. of Electricity, Uppsala University since 2002, with theobjective to develop full-scale wave power converters (WEC). Theconcept is based on a linear synchronous generator (anchored tothe seabed) driven by a heaving point absorber. This WEC has nogearbox or other mechanical or hydraulic conversion systems,resulting in a simpler and robust power plant. Since 2006, 12 suchWECs have been build and tested at the research site located atthe west coast of Sweden. The last update includes a new andextended project permit, deployment of a new marine substation,tests of several concepts of heaving buoys, grid connection,improved measuring station, improved modelling of wave powerfarms, implementation of remote operated vehicles forunderwater cable connection, and comprehensive environmentalmonitoring studies.
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