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Search: WFRF:(Strasser M)

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  • Beal, Jacob, et al. (author)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Journal article (peer-reviewed)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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  • Result 1-10 of 40
Type of publication
journal article (33)
conference paper (6)
research review (1)
Type of content
peer-reviewed (36)
other academic/artistic (4)
Author/Editor
Strasser, A (6)
Kumar, S (4)
Andersson, M (4)
Strasser, P. (4)
Wang, Y. (3)
Kaufmann, T (3)
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Pereira, DM (3)
Carmona-Gutierrez, D (3)
Madeo, F (3)
Kroemer, G (3)
Zhivotovsky, B (3)
Meier, P (3)
Wood, W (3)
Pagano, M (3)
Xu, H (3)
Fazekas, F. (3)
Bazan, NG (3)
Agostinis, P (3)
Simon, HU (3)
Klionsky, DJ (3)
Penninger, JM (3)
Cidlowski, JA (3)
Di Virgilio, F (3)
Uchida, H (3)
Puthalakath, H (3)
Piacentini, M (3)
Munoz-Pinedo, C. (3)
Rehm, M. (3)
Altucci, L (3)
Joseph, B (3)
Marine, JC (3)
Kepp, O (3)
Galluzzi, L (3)
Tavernarakis, N (3)
Rubinsztein, DC (3)
Beale, S. B. (3)
Pivovar, B.S. (3)
Mantz, R.A. (3)
Jones, D.J. (3)
Buechi, F. (3)
Fenton, J.M. (3)
Schmidt, T.J. (3)
Ayers, K.E. (3)
Weber, A.Z. (3)
Pintauro, P.N. (3)
Mitsushima, S. (3)
Stockwell, BR (3)
Conrad, M (3)
LIPTON, SA (3)
CAMPANELLA, M (3)
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University
Karolinska Institutet (23)
Lund University (8)
University of Gothenburg (6)
Stockholm University (4)
Uppsala University (3)
Chalmers University of Technology (3)
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University of Skövde (2)
Umeå University (1)
Royal Institute of Technology (1)
Linköping University (1)
Swedish University of Agricultural Sciences (1)
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Language
English (40)
Research subject (UKÄ/SCB)
Medical and Health Sciences (13)
Natural sciences (7)
Engineering and Technology (5)
Social Sciences (2)

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