| 1. |
- Stoor, Krister, 1959-
(author)
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Svenska kyrkan och jojken
- 2016. - 1
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In: De historiska relationerna mellan Svenska kyrkan och samerna. - Skellefteå : Artos & Norma bokförlag. - 9789175807959 ; , s. 711-734
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Book chapter (peer-reviewed)abstract
- Jojken är ett muntligt uttryck bland samer som funnits sedan urminnes tider. Att jojka var ett sätt att berätta och beskriva djur, människor, händelser, supranormala relationer etc. Eftersom jojken också användes som verktyg för nåjder eller personer med liknande kunskaper för att nå andra medium, blev jojken stämplad av kyrkan under 1600-talet som ett djävulens verk. Under lång tid var detta uttryckssätt förbjudet och straffbart. Men jojken försvann inte. Den levde sitt liv i det inofficiella rummet, långt borta ifrån den styrande eliten som oftast fanns vid kusten. Syndastämpeln som kyrkan så skickligt implanterat blev så småningom samernas egen syn på jojk på många håll. Trots det, när man studerar äldre inspelningar från början av 1910-talet och anteckningar från slutet av 1800-talet, ser man att många präster, särskilt i norra Västerbotten och södra Norrbotten varit mer än aktiva att dokumentera jojk. De har även blivit tillägnade egna melodier, det förnämsta man som jojkutövare någonsin kan skänka någon. Till och med bland læstadianska predikanter har det förekommit att man accepterat jojkning. "Jojkning är inte syndigt, blott man inte jojkar syndigt, liksom det ej heller är syndigt att dricka tvenne supar när det är kallt." Studien granskar jojkens roll hos den samiska befolkningen i relation till kyrkans folk, med fokus på 1900-talets utveckling.
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| 2. |
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| 3. |
- Dahlgaard Park, Su Mi, 1960-
(author)
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Kan arbetet bidra till livskvalitet?
- 2002
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In: Arbetsliv. - Stockholm : Prevent. - 1650-5220. ; :Nr. 3
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Journal article (pop. science, debate, etc.)
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| 4. |
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| 5. |
- Dalevi, Sören
(author)
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Strategi i barnbibelarbetet
- 2005
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In: 24tretton:Pedagogik i Svenska Kyrkan, nr 4 2005.
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Journal article (pop. science, debate, etc.)
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| 6. |
- Marigo, Valeria, et al.
(author)
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Modulation of cGMP-signalling to Prevent Retinal Degeneration
- 2019
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In: RSC Drug Discovery Series. - Cambridge : Royal Society of Chemistry. - 2041-3203. ; 2019-January:66, s. 88-98
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Journal article (peer-reviewed)abstract
- In the photoreceptors of the retina, the second-messenger molecule cyclic guanosine monophosphate (cGMP) occupies centre stage in the phototransduction cascade. Remarkably, cGMP is also involved in hereditary photoreceptor degeneration caused by a variety of different genetic insults. This provides an entry point for the development of inhibitory cGMP analogues for a mutation-independent treatment. Here, we outline how cGMP signalling can be targeted for the treatment of retinal degeneration, how inhibitory cGMP analogues may be designed and formulated, and how test systems of rising complexity can be used to identify new compounds with photoreceptor neuroprotective properties. In this context, we cite the European Union-funded DRUGSFORD project and provide an example for the efficacy of a specific cGMP analogue to prevent photoreceptor loss and preserve retinal function.
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| 7. |
- Bures, M., et al.
(author)
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On the Effectiveness of Combinatorial Interaction Testing : A Case Study
- 2017
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In: Proceedings - 2017 IEEE International Conference on Software Quality, Reliability and Security Companion, QRS-C 2017. - 9781538620724 ; , s. 69-76
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Conference paper (peer-reviewed)abstract
- Combinatorial interaction testing (CIT) stands as one of the efficient testing techniques that have been used in different applications recently. The technique is useful when there is a need to take the interaction of input parameters into consideration for testing a system. The key insight the technique is that not every single parameter may contribute to the failure of the system and there could be interactions among these parameters. Hence, there must be combinations of these input parameters based on the interaction strength. This technique has been used in many applications to assess its effectiveness. In this paper, we are addressing the effectiveness of CIT for a real-world case study using model-based mutation testing experiments. The contribution of the paper is threefold: First we introduce an effective testing application for CIT; Second, we address the effectiveness of increasing the interaction strength beyond the pairwise (i.e., interaction of more than two parameters); Third, model-based mutation testing is used to mutate the input model of the program in contrast to the traditional code-based mutation testing process. Experimental results showed that CIT is an effective testing technique for this kind of application. In addition, the results also showed the usefulness of model-based mutation testing to assess CIT applications. For the subject of this case study, the results also indicate that 3-way test suite (i.e., interaction of three parameters) could detect new faults that can not be detected by pairwise. © 2017 IEEE.
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| 8. |
- Chi, Celestine N., et al.
(author)
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Deciphering the kinetic binding mechanism of dimeric ligands, using a potent plasma-stable dimeric inhibitor of postsynaptic density protein-95 as an example
- 2010
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In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 285:36, s. 28252-28260
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Journal article (peer-reviewed)abstract
- Dimeric ligands can be potent inhibitors of protein-protein or enzyme-substrate interactions. They have increased affinity and specificity towards their targets due to their ability to bind simultaneously to two binding sites and are therefore very attractive in drug design. However, few studies have addressed the kinetic mechanism of interaction of such bivalent ligands. We have investigated the binding interaction of a recently identified potent plasma-stable dimeric pentapeptide of PDZ1-2 of PSD-95 using protein engineering in combination with fluorescence polarisation, isothermal titration calorimetry and stopped-flow fluorimetry. Our experiments demonstrate that binding occurs via a two-step process, where an initial binding to either one of the two PDZ domains is followed by an intramolecular step, which produces the bidentate complex. We have determined all rate constants involved in the binding reaction and we also find evidence for a conformational transition of the complex. Our data demonstrate the importance of a slow dissociation for a successful dimeric ligand, but also highlight the possibility of optimizing the intramolecular association rate. The results may therefore aid the design of dimeric inhibitors in general.
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| 9. |
- Chi, Celestine N., et al.
(author)
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Interactions outside the Boundaries of the Canonical Binding Groove of a PDZ Domain Influence Ligand Binding
- 2012
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In: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 51:44, s. 8971-8979
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Journal article (peer-reviewed)abstract
- The postsynaptic density protein-95/discs large/zonula occludens-1 (PDZ) domain is a protein-protein interaction module with a shallow binding groove where protein ligands bind. However, interactions that are not part of this canonical binding groove are likely to modulate peptide binding. We have investigated such interactions beyond the binding groove for PDZ3 from PSD-95 and a peptide derived from the C-terminus of the natural ligand CRIPT. We found via nuclear magnetic resonance experiments that up to eight residues of the peptide ligand interact with the PDZ domain, showing that the interaction surface extends far outside of the binding groove as defined by the crystal structure. PDZ3 contains an extra structural element, a C-terminal helix (α3), which is known to affect affinity. Deletion of this helix resulted in the loss of several intermolecular nuclear Overhauser enhancements from peptide residues outside of the binding pocket, suggesting that α3 forms part of the extra binding surface in wild-type PDZ3. Site-directed mutagenesis, isothermal titration calorimetry, and fluorescence intensity experiments confirmed the importance of both α3 and the N-terminal part of the peptide for the affinity. Our data suggest a general mechanism in which different binding surfaces outside of the PDZ binding groove could provide sites for specific interactions.
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| 10. |
- Chi, Celestine N., et al.
(author)
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NOE-derived methyl distances from a 360 kDa proteasome complex
- 2018
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In: Chemistry - A European Journal. - : Wiley. - 0947-6539 .- 1521-3765. ; 24:9, s. 2270-2276
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Journal article (peer-reviewed)abstract
- Nuclear magnetic resonance spectroscopy is the prime tool to probe structure and dynamics of biomolecules at atomic resolution. A serious challenge for that method is the size limit imposed on molecules to be studied. Standard studies are typically restricted to ca. 30-40 kDa. More recent developments lead to spin relaxation measurements in methyl groups in single proteins or protein complexes as large as a mega-Dalton, which directly allow the extraction of angular information or experiments with paramagnetic samples. However, these probes are all of indirect nature in contrast to the most intuitive and easy-to-interpret structural/dynamics restraint, the internuclear distance, which can be measured by nuclear Overhauser enhancement (NOE). Here, we demonstrate time-averaged distance measurements on the 360 kDa half proteasome from Thermoplasma acidophilium. The approach is based on exact quantification of the NOE (eNOE). Our findings open up an avenue for such measurements on very large molecules. These restraints will help in a detailed determination of conformational changes upon perturbation such as ligand binding.
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