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- Escamez, Sacha, 1987-, et al.
(author)
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Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis
- 2020
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In: Current Biology. - : Elsevier. - 0960-9822 .- 1879-0445. ; 30:3, s. 455-464
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Journal article (peer-reviewed)abstract
- Plant organ growth is widely accepted to be determined by cell division and cell expansion, but, unlike that in animals, the contribution of cell elimination has rarely been recognized. We investigated this paradigm during Arabidopsis lateral root formation, when the lateral root primordia (LRP) must traverse three overlying cell layers within the parent root. A subset of LRP-overlying cells displayed the induction of marker genes for cell types undergoing developmental cell death, and their cell death was detected by electron, confocal, and light sheet microscopy techniques. LRP growth was delayed in cell-deathdeficient mutants lacking the positive cell death regulator ORESARA1/ANAC092 (ORE1). LRP growth was restored in ore1-2 knockout plants by genetically inducing cell elimination in cells overlying the LRP or by physically killing LRP-overlying cells by ablation with optical tweezers. Our results support that, in addition to previously discovered mechanisms, cell elimination contributes to regulating lateral root emergence.
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| 2. |
- Sztojka, Bernadette, 1990-
(author)
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New regulators of xylem lignification in arabidopsis
- 2020
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Doctoral thesis (other academic/artistic)abstract
- The ability of land plants to grow upright, bear their own weight and withstand adverse environmental conditions is largely dependent on the secondary xylem tissues of the stem. The xylem cells acquire thick secondary cell walls which are composed of cellulose, hemicellulose and lignin. The chemical structure of lignin renders the secondary cell wall rigid and waterproof, facilitating the transport of water and solutes through the vascular system. Lignin is a polyphenolic polymer composed of three different types of lignin units, guaiacyl (G), syringyl (S) and p-hydroxyphenyl (H), derived from the coniferyl, sinapyl and p-coumaryl alcohol, respectively. Lignin biosynthesis, monolignol transport and lignin polymerization (collectively called as ”lignification”) are controlled by numerous transcription factors and other regulators.This thesis work uncovers three novel regulators of lignification in the secondary xylem tissues of Arabidopsis (Arabidopsis thaliana) stem and hypocotyl. The cupin domain containing protein PIRIN2 (PRN2) suppresses S-type lignin accumulation. PRN2 functions in a non-cell-autonomous fashion: it is expressed in the cells next to the xylem vessel elements, but affects the lignin composition of the vessel and fiber cell walls of the neighbouring cells. Two protein interactors of PRN2 are characterized here in connection to lignification. Opposite to the function of PRN2, the chromatin-modifying protein HISTONE MONOUBIQUITINATION2 (HUB2) promotes S-type lignin deposition. In line with this, PRN2 and HUB2 antagonistically regulate the expression of FERULATE-5-HYDROXYLASE1 which encodes the key S-type lignin-biosynthetic enzyme. Possibly, PRN2 antagonizes the S-lignin promoting function of HUB2 to ensure that the cell walls of the vessel elements get enriched in G-type lignin. Finally, identification of a potential diurnal modulator of lignin biosynthesis is described in this work. The PRN2-interacting basic helix-loop-helix transcription factor (PIB) does not influence the lignin content or composition of the secondary cell walls. However, PIB affects the diurnal expression pattern and promoter activity of some lignin-biosynthetic genes. Altogether, PRN2, HUB2 and PIB highlight the importance of intercellular co-operation in lignification, and uncover novel regulatory aspects of this process.
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| 3. |
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| 4. |
- Zhang, Bo, et al.
(author)
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PIRIN2 suppresses S-type lignin accumulation in a noncell-autonomous manner in Arabidopsis xylem elements
- 2020
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In: New Phytologist. - : New Phytologist Trust. - 0028-646X .- 1469-8137. ; 225:5, s. 1923-1935
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Journal article (peer-reviewed)abstract
- PIRIN (PRN) genes encode cupin domain‐containing proteins that function as transcriptional co‐regulators in humans but that are poorly described in plants. A previous study in xylogenic cell cultures of Zinnia elegans suggested a role for a PRN protein in lignification. This study aimed to identify the function of Arabidopsis (Arabidopsis thaliana) PRN proteins in lignification of xylem tissues.Chemical composition of the secondary cell walls was analysed in Arabidopsis stems and/or hypocotyls by pyrolysis–gas chromatography/mass spectrometry, 2D‐nuclear magnetic resonance and phenolic profiling. Secondary cell walls of individual xylem elements were chemotyped by Fourier transform infrared and Raman microspectroscopy.Arabidopsis PRN2 suppressed accumulation of S‐type lignin in Arabidopsis stems and hypocotyls. PRN2 promoter activity and PRN2:GFP fusion protein were localised specifically in cells next to the vessel elements, suggesting a role for PRN2 in noncell‐autonomous lignification of xylem vessels. Accordingly, PRN2 modulated lignin chemistry in the secondary cell walls of the neighbouring vessel elements.These results indicate that PRN2 suppresses S‐type lignin accumulation in the neighbourhood of xylem vessels to bestow G‐type enriched lignin composition on the secondary cell walls of the vessel elements. Gene expression analyses suggested that PRN2 function is mediated by regulation of the expression of the lignin‐biosynthetic genes.
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| 5. |
- Zhang, Bo, et al.
(author)
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The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels
- 2020
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In: Journal of Experimental Botany. - : Oxford University Press. - 0022-0957 .- 1460-2431. ; 71:18, s. 5484-5494
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Journal article (peer-reviewed)abstract
- PIRIN2 (PRN2) was earlier reported to suppress syringyl (S)-type lignin accumulation of xylem vessels of Arabidopsis thaliana. In the present study, we report yeast two-hybrid results supporting the interaction of PRN2 with HISTONE MONOUBIQUITINATION2 (HUB2) in Arabidopsis. HUB2 has been previously implicated in several plant developmental processes, but not in lignification. Interaction between PRN2 and HUB2 was verified by β-galactosidase enzymatic and co-immunoprecipitation assays. HUB2 promoted the deposition of S-type lignin in the secondary cell walls of both stem and hypocotyl tissues, as analysed by pyrolysis-GC/MS. Chemical fingerprinting of individual xylem vessel cell walls by Raman and Fourier transform infrared microspectroscopy supported the function of HUB2 in lignin deposition. These results, together with a genetic analysis of the hub2 prn2 double mutant, support the antagonistic function of PRN2 and HUB2 in deposition of S-type lignin. Transcriptome analyses indicated the opposite regulation of the S-type lignin biosynthetic gene FERULATE-5-HYDROXYLASE1 by PRN2 and HUB2 as the underlying mechanism. PRN2 and HUB2 promoter activities co-localized in cells neighbouring the xylem vessel elements, suggesting that the S-type lignin-promoting function of HUB2 is antagonized by PRN2 for the benefit of the guaiacyl (G)-type lignin enrichment of the neighbouring xylem vessel elements.
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