| 1. |
- Unnerstad, Helle, et al.
(author)
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Listeria monocytogenes in faeces from clinically healthy dairy cows in Sweden
- 2000
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In: Acta Veterinaria Scandinavica. - 0044-605X .- 1751-0147. ; 41:2, s. 167-171
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Journal article (peer-reviewed)abstract
- Faecal samples from 102 clinically healthy dairy cows, representing 34 farms in the Swedish province of Uppsala, were analysed for the presence of Listeria spp. using an enrichment procedure. Listeria monocytogenes was isolated from six (6%) and L. innocua from 2 (2%) cows. From each of the 6 samples positive for L. monocytogenes, 5 isolates were further characterised by restriction enzyme analysis using the 3 enzymes Apa I, Sma I, and Asc I, followed by pulsed-field gel electrophoresis. Three of the L. monocytogenes positive cows lived at the same farm, and they all harboured the same clonal type. One of these 3 cows also harboured a further clonal type of L. monocytogenes. The fact that one of the cows harboured 2 different clonal types of L. monocytogenes is important from an epidemiological point of view when routes of infection are to be investigated.
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| 2. |
- Brown, Phillip, et al.
(author)
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Investigation of a Listeria monocytogenes Chromosomal Immigration Control Region Reveals Diverse Restriction Modification Systems with Complete Sequence Type Conservation
- 2023
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In: Microorganisms. - : MDPI. - 2076-2607. ; 11:3
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Journal article (peer-reviewed)abstract
- Listeria monocytogenes is a Gram-positive pathogen responsible for the severe foodborne disease listeriosis. A chromosomal hotspot between lmo0301 and lmo0305 has been noted to harbor diverse restriction modification (RM) systems. Here, we analyzed 872 L. monocytogenes genomes to better understand the prevalence and types of RM systems in this region, designated the immigration control region (ICR). Type I, II, III and IV RM systems were found in 86.1% of strains inside the ICR and in 22.5% of strains flanking the ICR. ICR content was completely conserved within the same multilocus sequence typing-based sequence type (ST), but the same RM system could be identified in diverse STs. The intra-ST conservation of ICR content suggests that this region may drive the emergence of new STs and promote clone stability. Sau3AI-like, LmoJ2 and LmoJ3 type II RM systems as well as type I EcoKI-like, and type IV AspBHI-like and mcrB-like systems accounted for all RM systems in the ICR. A Sau3AI-like type II RM system with specificity for GATC was harbored in the ICR of many STs, including all strains of the ancient, ubiquitous ST1. The extreme paucity of GATC recognition sites in lytic phages may reflect ancient adaptation of these phages to preempt resistance associated with the widely distributed Sau3AI-like systems. These findings indicate that the ICR has a high propensity for RM systems which are intraclonaly conserved and may impact bacteriophage susceptibility as well as ST emergence and stability.
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| 6. |
- Danielsson-Tham, Marie-Louise, 1947-, et al.
(author)
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A fatal case of Listeria endocarditis in a man following his tending of goats suggests an epidemiological link which is not supported by the results
- 1997
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In: Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health. - : Wiley. - 0931-1793 .- 1439-0450. ; B 44:1-10, s. 253-256
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Journal article (peer-reviewed)abstract
- A man died in endocarditis due to listeriosis in the late autumn. He had been looking after two goats during the summer. Listeria monocyto?enes was isolated from a rectal swab from one of the goats. The goat faeces isolate and the human blood isolate were of identical serovar. The two isolates, however, were shown to be different by multilocus electrophoretic enzyme analysis and ribotyping, as well as by biotyping, Thus, these results do not support the hypothesis that the man was infected by the goat.
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| 7. |
- Danielsson-Tham, Marie-Louise, 1947-, et al.
(author)
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Causes behind a human cheese-borne outbreak of gastrointestinal listeriosis
- 2004
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In: Foodborne pathogens and disease. - New York, USA : Mary Ann Liebert. - 1535-3141 .- 1556-7125. ; 1:3, s. 153-159
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Journal article (peer-reviewed)abstract
- In a previous paper, we reported an outbreak of gastrointestinal listeriosis due to consumption of fresh cheese made from raw milk and manufactured on a summer farm. The aim of the present study was to investigate why the cheese harbored Listeria monocytogenes. To our knowledge, this is the first documented outbreak of listeriosis caused by raw milk cheese where the human epidemic strain has been cultured from a dairy animal, whose milk has been used for cheese production. The conditions on a summer farm can hardly fulfil the requirements for hygienic and strictly controlled conditions necessary for safe processing of fresh cheese.
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