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  • Gonzalez Rodriguez, Adrian, 1988, et al. (author)
  • Melting transition of oriented Li-DNA fibers submerged in ethanol solutions
  • 2021
  • In: Biopolymers. - : Wiley. - 0006-3525 .- 1097-0282. ; 112:3
  • Journal article (peer-reviewed)abstract
    • The melting transition of Li-DNA fibers immersed in ethanol-water solutions has been studied using calorimetry and neutron diffraction techniques. The data have been analyzed using the Peyrard-Bishop-Dauxois model to determine the strengths of the intra- and inter-base pair potentials. The data and analysis show that the potentials are weaker than those for DNA in water. They become weaker still and the DNA less stable as the ethanol concentration increases but, conversely, the fibers become more compact and the distances between base pairs become more regular. The results show that the melting transition is relatively insensitive to local confinement and depends more on the interaction between the DNA and its aqueous environment.
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