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- Andersson, M, et al.
(author)
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Disease-related variations of the glycosylation of haptoglobin in the dog
- 1998
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In: Journal of Comparative Pathology. - 1532-3129. ; 119:3, s. 227-238
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Journal article (peer-reviewed)abstract
- Haptoglobin phenotypes have been shown in human medicine to be related to the prevalence of various diseases. Furthermore, abnormal glycosylation of haptoglobin has been reported as a consequence of liver disease, cancer and immunological disorders in man. To our knowledge, similar findings have not, so far, been reported in canine disease. The present paper describes a method for investigation of canine haptoglobin phenotypes and of microheterogeneity caused by altered glycosylation. The method consisted of isoelectric focusing (IEF) of dog serum, followed by immunoblotting. The results indicated the existence of only one canine haptoglobin phenotype with a characteristic microheterogeneity pattern in healthy dogs. Changes in this pattern were found in serum from dogs with liver disease, predominantly chronic progressive hepatitis, and with different kinds of anaemia. Pretreatment of serum with neuraminidase or glycopeptidase F (PNGase F) resulted in identical IEF patterns of haptoglobin from healthy and diseased dogs. Moreover, a fucose-specific lectin was capable of binding to some of the abnormal haptoglobin fractions, mainly those found in association with anaemia. The changes described were interpreted as alterations of the carbohydrate content, with or without fucosylation, of some haptoglobin fractions.
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| 2. |
- Boon, Wietse M., et al.
(author)
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Functional analysis and exterior calculus on mixed-dimensional geometries
- 2020
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In: Annali di Matematica Pura ed Applicata. - : Springer. - 0373-3114 .- 1618-1891.
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Journal article (peer-reviewed)abstract
- We are interested in differential forms on mixed-dimensional geometries, in the sense of a domain containing sets of d-dimensional manifolds, structured hierarchically so that each d-dimensional manifold is contained in the boundary of one or more d+ 1 -dimensional manifolds. On any given d-dimensional manifold, we then consider differential operators tangent to the manifold as well as discrete differential operators (jumps) normal to the manifold. The combined action of these operators leads to the notion of a semi-discrete differential operator coupling manifolds of different dimensions. We refer to the resulting systems of equations as mixed-dimensional, which have become a popular modeling technique for physical applications including fractured and composite materials. We establish analytical tools in the mixed-dimensional setting, including suitable inner products, differential and codifferential operators, Poincaré lemma, and Poincaré–Friedrichs inequality. The manuscript is concluded by defining the mixed-dimensional minimization problem corresponding to the Hodge Laplacian, and we show that this minimization problem is well-posed.
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