| 1. |
- Ahlkvist, Linda, et al.
(author)
-
Synergism by individual macronutrients explains the marked early GLP-1 and islet hormone responses to mixed meal challenge in mice
- 2012
-
In: Regulatory Peptides. - : Elsevier. - 0167-0115 .- 1873-1686. ; 178:1-3, s. 29-35
-
Journal article (peer-reviewed)abstract
- Apart from glucose, proteins and lipids also stimulate incretin and islet hormone secretion. However, the glucoregulatory effect of macronutrients in combination is poorly understood. We therefore developed an oral mixed meal model in mice to 1) explore the glucagon-like peptide-1 (GLP-1) and islet hormone responses to mixed meal versus isocaloric glucose, and 2) characterize the relative contribution of individual macronutrients to these responses. Anesthetized C57BL/6J female mice were orally gavaged with 1) a mixed meal (0.285 kcal; glucose, whey protein and peanut oil; 60/20/20% kcal) versus an isocaloric glucose load (0.285 kcal), and 2) a mixed meal (0.285 kcal) versus glucose, whey protein or peanut oil administered individually in their mixed meal caloric quantity, i.e., 0.171, 0.055 and 0.055 kcal, respectively. Plasma was analyzed for glucose, insulin and intact GLP-1 before and during oral challenges. Plasma glucose was lower after mixed meal versus after isocaloric glucose ingestion. In spite of this, the peak insulin response (P=0.02), the peak intact GLP-1 levels (P=0.006) and the estimated β-cell function (P=0.005) were higher. Furthermore, the peak insulin (P=0.004) and intact GLP-1 (P=0.006) levels were higher after mixed meal ingestion than the sum of responses to individual macronutrients. Compared to glucose alone, we conclude that there is a marked early insulin response to mixed meal ingestion, which emanates from a synergistic, rather than an additive, effect of the individual macronutrients in the mixed meal and is in part likely caused by increased levels of GLP-1.
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Andersson, Sofia A, et al.
(author)
-
Glucose-dependent docking and SNARE protein-mediated exocytosis in mouse pancreatic alpha-cell
- 2011
-
In: Pflügers Archiv. - : Springer. - 0031-6768 .- 1432-2013. ; 462:3, s. 443-454
-
Journal article (peer-reviewed)abstract
- The function of alpha-cells in patients with type 2 diabetes is often disturbed; glucagon secretion is increased at hyperglycaemia, yet fails to respond to hypoglycaemia. A crucial mechanism behind the fine-tuned release of glucagon relies in the exocytotic machinery including SNARE proteins. Here, we aimed to investigate the temporal role of syntaxin 1A and SNAP-25 in mouse alpha-cell exocytosis. First, we used confocal imaging to investigate glucose dependency in the localisation of SNAP-25 and syntaxin 1A. SNAP-25 was mainly distributed in the plasma membrane at 2.8 mM glucose, whereas the syntaxin 1A distribution in the plasma membrane, as compared to the cytosolic fraction, was highest at 8.3 mM glucose. Furthermore, following inclusion of an antibody against SNAP-25 or syntaxin 1A, exocytosis evoked by a train of ten depolarisations and measured as an increase in membrane capacitance was reduced by ~50%. Closer inspection revealed a reduction in the refilling of granules from the reserve pool (RP), but also showed a decreased size of the readily releasable pool (RRP) by ~45%. Disparate from the situation in pancreatic beta-cells, the voltage-dependent Ca²⁺ current was not reduced, but the Ca²⁺ sensitivity of exocytosis decreased by the antibody against syntaxin 1A. Finally, ultrastructural analysis revealed that the number of docked granules was >2-fold higher at 16.7 mM than at 1 mM glucose. We conclude that syntaxin 1A and SNAP-25 are necessary for alpha-cell exocytosis and regulate fusion of granules belonging to both the RRP and RP without affecting the Ca²⁺ current.
|
|
| 5. |
- Bjärsholm, Daniel, et al.
(author)
-
A longitudinal study on exercise habits and mental health among Swedish police students
- 2023
-
Conference paper (peer-reviewed)abstract
- IntroductionIn Sweden, police education should promote students’ physical activity and mental health. According to national goals, police students should be provided with sufficient conditions to develop and maintain advantageous exercise habits and tools to handle various mentally and physically demanding tasks. The national goals also state that students’ physical fitness must be better at graduation than what the requirements are for admission (see Krugly et al., 2022). Although the improvement of students’ physical fitness and mental health are national goals, there is a general lack of knowledge regarding: 1) police students physical and mental health, especially from a Swedish perspective; and 2) how well police education promotes students’ level of physical activity during education. Against this background, the aim is to explore police students’ mental health and level of physical activity during police education in Sweden.MethodsThe data derives from the largest project in Sweden on police students’ physical and mental health, and consists of longitudinal data on police students answers of a self-rated questionnaire about exercise habits and mental health (N = 785). The data used in this study was gathered between 2019–2021, and consisted of four data collection points, from two police educations in Sweden. The analysis was conducted in two steps. First, exploratory- and confirmatory factor analyses were conducted to create scales for mental health orientation. Second, these scales, together with the variable exercise habits, were used as outcome variables in t-tests, X2 test and ANOVA. Effect size measurements were calculated and interpreted based on established guidelines.ResultsThe results show high psychometric support for two scales named positive health orientation and negative health orientation. Based on the scales and the variable of exercise habits, three primarily results emerge: 1) the levels of physical training for men decrease during education; 2) there are gender differences showing that women have a more negative health orientation; and, 3) the positive mental health orientation decreases during education for both men and women.DiscussionSwedish police education should prepare students for physically and mentally demanding work. However, as this study concludes, this tends to not be the case, especially given that both the level of physical activity and the positive mental health orientation decrease during education. This raises questions on whether the Swedish police education is doing “enough” to provide the students with adequate conditions for improving their mental and physical health.Krugly, S., Bjärsholm, D., Jansson, A., Rosendal Hansen, A., Hansson, O., Brehm, K., Datmo, A., Hafsteinsson Östenberg, A., & Vikman, J. (2022). A retrospective study of physical fitness and mental health among police students in Sweden. The Police Journal: Theory, Practice and Principles. doi.org/10.1177/0032258X221089576
|
|
| 6. |
- Carr, Richard D, et al.
(author)
-
Secretion and Dipeptidyl Peptidase-4-Mediated Metabolism of Incretin Hormones after a Mixed Meal or Glucose Ingestion in Obese Compared to Lean, Nondiabetic Men.
- 2010
-
In: The Journal of clinical endocrinology and metabolism. - : The Endocrine Society. - 1945-7197 .- 0021-972X. ; 95, s. 872-878
-
Journal article (peer-reviewed)abstract
- Context: Glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are cleaved by dipeptidyl peptidase-4 (DPP-4); plasma activity of DPP-4 may be increased in obesity. The impact of this increase on incretin hormone secretion and metabolism is not known. Objective: The aim of the study was to assess incretin hormone secretion and degradation in lean and obese nondiabetic subjects. Design, Settings, and Participants: We studied the ingestion of a mixed meal (560 kcal) or oral glucose (2 g/kg) in healthy lean (n = 12; body mass index, 20-25 kg/m(2)) or obese (n = 13; body mass index, 30-35 kg/m(2)) males at a University Clinical Research Unit. Main Outcome Measures: We measured the area under the curve of plasma intact (i) and total (t) GIP and GLP-1 after meal ingestion and oral glucose. Results: Plasma DPP-4 activity was higher in the obese subjects (38.5 +/- 3.0 vs. 26.7 +/- 1.6 mmol/min . mul; P = 0.002). Although GIP secretion (AUCtGIP) was not reduced in obese subjects after meal ingestion or oral glucose, AUCiGIP was lower in obese subjects (8.5 +/- 0.6 vs. 12.7 +/- 0.9 nmol/liter x 300 min; P < 0.001) after meal ingestion. GLP-1 secretion (AUCtGLP-1) was reduced in obese subjects after both meal ingestion (7.3 +/- 0.9 vs. 10.0 +/- 0.6 nmol/liter x 300 min; P = 0.022) and oral glucose (6.6 +/- 0.8 vs. 9.6 +/- 1.1 nmol/liter x 180 min; P = 0.035). iGLP-1 was reduced in parallel to tGLP-1. Conclusions: 1) Release and degradation of the two incretin hormones show dissociated changes in obesity: GLP-1 but not GIP secretion is lower after meal ingestion and oral glucose, whereas GIP but not GLP-1 metabolism is increased after meal ingestion. 2) Increased plasma DPP-4 activity in obesity is not associated with a generalized augmented incretin hormone metabolism.
|
|
| 7. |
- Jansson, Alexander, et al.
(author)
-
Mental health and exercise habits among police students in Sweden : a three-year retrospective study
- 2023
-
In: Abstracts from the First European Conference on Law Enforcement and Public Health, Umeå 2023. - : Enheten för polisutbildning, Umeåuniversitet. - 9789180701099
-
Conference paper (other academic/artistic)abstract
- Working as a police officer involves mentally and physically demanding tasks. During the Swedish police education, students should be provided with sufficient conditions to develop and maintain advantageous exercise habits and tools to handle mentally and physically demanding tasks (see Krugly et al., 2022). However, there is a lack of knowledge regarding Swedish police students exercise habits and overall mental- and physical health.The aim is to explore police students’ mental health and physical activity levels during police education in Sweden.The data consisted of police students answers of a self-rated questionnaire about their physical and mental health. The data used in this study was gathered between 2019–2021, and consist of four data collection points, from two police educations in Sweden. The analysis was conducted in two steps. First, exploratory- and confirmatory factor analyses were conducted to create scales for health orientation. Second, these scales were used as outcome variables in t-tests, X2 test and ANOVA. Effect size measurements (Cohens, d, Crames V and Phi) were calculated and interpreted based on well establish guidelines.Two scales were developed (i.e. positive health orientation and negative health orientation), and both showed high psychometric support. Based on the scales, two primarily results emerged: 1) results showed that women had a more negative health orientation in general and that positive health orientation, for both genders, decreased between semesters one to four; and 2) more women conducted two hours (or more) per week of physical exercise. Moreover, physical training among men decreased continuously during their education.Conclusions and ImplicationsBased on the results, this study questions whether police education in Sweden doing enough to prepare students for a mentally and physically demanding profession.
|
|
| 8. |
- Jansson, Alexander, et al.
(author)
-
Mental health and exercise habits among police students in Sweden : A three-year retrospective study
- 2024
-
In: The Police Journal. - : Sage Publications. - 0032-258X .- 1740-5599. ; 97:3, s. 401-416
-
Journal article (peer-reviewed)abstract
- This study aims to explore police students’ self-rated mental health and physical activity levels and the relationship between them. Based on longitudinal and cross-sectional data (N = 722), two scales on mental health orientation were developed. The scales and levels of physical activity were analyzed using t-test, ANOVA, and X2. During police education, (1) the level of physical activity decreases for men, (2) there is a decline in positive health orientation for both sexes, and (3) women report a more negative health orientation. This raises questions regarding whether “enough” is done to provide police students with sufficient conditions for improving their health.
|
|
| 9. |
- Jansson, Alexander, et al.
(author)
-
Mental health and exercise habits among police students in Sweden : A three-year retrospective study
- 2023
-
In: The Police Journal. - : Sage Publications. - 0032-258X .- 1740-5599. ; , s. 1-16
-
Journal article (peer-reviewed)abstract
- This study aims to explore police students’ self-rated mental health and physical activity levels and the relationship between them. Based on longitudinal and cross-sectional data (N = 722), two scales on mental health orientation were developed. The scales and levels of physical activity were analyzed using t-test, ANOVA, and X2. During police education, (1) the level of physical activity decreases for men, (2) there is a decline in positive health orientation for both sexes, and (3) women report a more negative health orientation. This raises questions regarding whether “enough” is done to provide police students with sufficient conditions for improving their health.
|
|
| 10. |
- Jansson, Alexander, et al.
(author)
-
Träningsråd till dig som ska genomföra tester av fysisk förmåga på Plikt- och prövningsverket
- 2023
-
Reports (other academic/artistic)abstract
- På Plikt- och prövningsverket genomförs flera olika tester. När det kommer till att testa de prövandes fysiska förmåga används bland annat ett konditionstest på cykel och ett isokinetiskt styrketest som kallas Isokai. I den här texten ges information om testerna, grundläggande träningsråd för att klara dessa samt råd inför fortsatt träning. Råden är uppdelade i två delar. I den första delen beskrivs träningsråd för styrketräning och i den andra för konditionsträning.
|
|
