| 1. |
- Du, Yaoyao, et al.
(author)
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Cartilage Oligomeric Matrix Protein Inhibits Vascular Smooth Muscle Calcification by Interacting With Bone Morphogenetic Protein-2.
- 2011
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In: Circulation Research. - 1524-4571. ; 108, s. 79-917
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Journal article (peer-reviewed)abstract
- Rationale: Vascular calcification is a significant contributor to cardiovascular morbidity and mortality. We recently reported that cartilage oligomeric matrix protein (COMP) is pivotal for maintaining the homeostasis of vascular smooth muscle cells (VSMCs). Whether COMP affects the process of vascular calcification is unknown. Objective: We aimed to test whether COMP modulates vascular calcification. Methods and Results: VSMC calcification in vitro was induced by calcifying media containing high inorganic phosphate or calcium. In vivo medial vessel calcification was induced in rats by 5/6 nephrectomy with a high-phosphate diet or by periadventitial application of CaCl(2) to the abdominal aorta. COMP protein level was markedly reduced in both calcified VSMCs and arteries. COMP deficiency remarkably exacerbated VSMC calcification, whereas ectopic expression of COMP greatly reduced calcification. Furthermore, COMP knockdown facilitated osteogenic markers expression by VSMCs even in the absence of calcifying media. By contrast, COMP overexpression significantly inhibited high phosphate- or high calcium-induced VSMC osteochondrogenic transition. Induction of osteogenic marker expression by COMP silencing was reversed by a soluble form of bone morphogenetic protein (BMP)-2 receptor IA, which suggests a BMP-2-dependent mechanism. Our data revealed that COMP bound directly to BMP-2 through the C terminus, inhibited BMP-2 receptor binding, and blocked BMP-2 osteogenic signaling, indicating COMP inhibits osteochondrogenic transition of VSMCs at least partially through inhibiting BMP-2. Conclusions: Our data strongly suggest that COMP is a novel inhibitor of vascular calcification. The imbalance between the effects of COMP and BMP-2 may provide new insights into the pathophysiology of vascular calcification.
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| 2. |
- Liu, Qingbo, et al.
(author)
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Effects of carbon pre-germanidation implant into Ge on the thermal stability of NiGe films
- 2015
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In: Microelectronic Engineering. - : Elsevier BV. - 0167-9317 .- 1873-5568. ; 133, s. 6-10
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Journal article (peer-reviewed)abstract
- In this work, the effects of carbon pre-germanidation implant into Ge on the properties of NiGe films were systematically investigated. NiGe films with carbon pre-germanidation implant to doses varying from 0 to 6 x 10(15) cm(-2) were characterized by means of sheet resistance measurement, X-ray diffraction (XRD), scanning electron microscopy (SEM), cross-sectional transmission electron microscope (X-TEM) and secondary ion mass spectroscopy (SIMS). The presence of C atoms is proved to significantly enhance the thermal stability of NiGe by about 100 degrees C as well as to change the preferred orientations of polycrystalline NiGe. The homogenous redistribution of C atoms within NiGe films and the segregation of C atoms at the NiGe/Ge interface is responsible for the improved thermal stability of NiGe films.
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| 3. |
- Liu, Qingbo, et al.
(author)
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Effects of Carbon Pre-Germanidation Implantation on the Thermal Stability of NiGe and Dopant Segregation on Both n- and p-Type Ge Substrate
- 2015
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In: ECS Journal of Solid State Science and Technology. - : The Electrochemical Society. - 2162-8769 .- 2162-8777. ; 4:5, s. P119-P123
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Journal article (peer-reviewed)abstract
- In this work, the effects of carbon pre-geramanidation implantation on the thermal stability of NiGe and dopant segregation on both ntype and p-type Ge substrate were investigated systematically. As-prepared NiGe films with carbon pre-germanidation implantation to different doses were characterized by means of sheet resistance measurement, X-ray diffraction (XRD), scanning electron microscopy (SEM), cross-sectional transmission electron microscope (X-TEM) and secondary ion mass spectroscopy (SIMS). The presence of carbon is proved to improve the thermal stability of NiGe formed on both n-and p-type Ge significantly, as well as to lead to dopant segregation (DS) of P and B at the NiGe/Ge interface. The homogeneous distribution of C within NiGe films and stuffing of C atoms at the NiGe/Ge interface is responsible for the enhanced thermal stability of NiGe and DS of P and B during germanidation process. (C) The Author(s) 2015. Published by ECS. All rights reserved.
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| 4. |
- Liu, Qingbo, et al.
(author)
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Improvement of the Thermal Stability of Nickel Stanogermanide by Carbon Pre-Stanogermanidation Implant into GeSn Substrate
- 2015
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In: ECS Journal of Solid State Science and Technology. - : The Electrochemical Society. - 2162-8769 .- 2162-8777. ; 4:3, s. P67-P70
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Journal article (peer-reviewed)abstract
- An effective method to improve the thermal stability of Ni(Ge1-xSnx) by carbon pre-stanogermanidation implant into GeSn substrate is investigated systematically. As-prepared samples were characterized by means of sheet resistance measurement, X-ray diffraction (XRD), scanning electron microscopy (SEM), cross-sectional transmission electron microscopy (X-TEM) and secondary ions mass spectroscopy (SIMS). The incorporation of carbon leads to significantly improved thermal stability of Ni(Ge1-xSnx) by about 100 degrees C as well as tends to change the preferred orientations of polycrystalline Ni(Ge1-xSnx). The robust thermal stability can be attributed to the segregation of C in grain boundaries and at Ni(Ge1-xSnx)/GeSn interface after stanogermanidation.
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| 5. |
- Wang, Qingbo, et al.
(author)
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Landscape of multi-nucleotide variants in 125,748 human exomes and 15,708 genomes
- 2020
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 11
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Journal article (peer-reviewed)abstract
- Multi-nucleotide variants (MNVs), defined as two or more nearby variants existing on the same haplotype in an individual, are a clinically and biologically important class of genetic variation. However, existing tools typically do not accurately classify MNVs, and understanding of their mutational origins remains limited. Here, we systematically survey MNVs in 125,748 whole exomes and 15,708 whole genomes from the Genome Aggregation Database (gnomAD). We identify 1,792,248 MNVs across the genome with constituent variants falling within 2 bp distance of one another, including 18,756 variants with a novel combined effect on protein sequence. Finally, we estimate the relative impact of known mutational mechanisms - CpG deamination, replication error by polymerase zeta, and polymerase slippage at repeat junctions - on the generation of MNVs. Our results demonstrate the value of haplotype-aware variant annotation, and refine our understanding of genome-wide mutational mechanisms of MNVs
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